LOCUS       sequence-82450-e        1104 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses CcaS and CcaR constitutively, and sfGFP under the PcpcG2 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1104)
  AUTHORS   Olson EJ, Hartsough LA, Landry BP, Shroff R, Tabor JJ
  TITLE     Characterizing bacterial gene circuit dynamics with optically 
            programmed gene expression signals.
  JOURNAL   Nat Methods. 2014 Mar 9. doi: 10.1038/nmeth.2884.
  PUBMED    24608181
REFERENCE   2  (bases 1 to 1104)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2014 Mar 9. doi: 10.1038/nmeth.2884."
COMMENT     Sequence Label: pJT119b
FEATURES             Location/Qualifiers
     source          1..1104
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(10..96)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     RBS             1051..1062
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 gggcggcgga tttgtcctac tcaggagagc gttcaccgac aaacaacaga taaaacgaaa
       61 ggcccagtct ttcgactgag cctttcgttt tatttgatgc ctctagacta gtttttccct
      121 tggcacaaag atttttccgt taaattggcc agacgatacc caatgccatg gaccgtttca
      181 atggcatctg ctgataaacc ggcacttttt aatttttgtc gcaaactccg cacatgcacc
      241 ttaaccgtat cttcctctgg gggactctcc aacttccaga tactatcgat aatcatgctc
      301 cgacttagca cccgacggcc attgcggagt agtaattcca gaatgctgta ttccttgcgg
      361 gtcaaagaca aaacctcatt gtcataacta acttcatagg tgcttggatc caacctgatt
      421 ggcccccact ctaaaactgg ttggcacgtt gcacaacccc gacgcaacaa agctcgcacc
      481 ctggcaaata actcccccaa atccactggc ttgaccacat aatcatccgc ccccgcatcc
      541 aagcccgtga ttttatcatt gatcgtatcc ctggctgtca tcatcaaaat tggcattaaa
      601 taactgtgcg atcgccattt ttgacagagg gtaatcccgt ccaactccgg cagcatcaca
      661 tccaaaataa cgaggtcata ttccagtcgg gaggcatagt cccaggcgag ggaagcgtcg
      721 gtggcaatat caacggtgta aagctggtca ctcaatgctt cagcaagggt ttccgccagc
      781 ggcaaatcat cctccactaa aagaattctc atagcccatt gtgcttttct ctatcaacct
      841 cagcttacct gaaggggtga acaggtctgg gttaattcat gttgcgaaat gtaacagttt
      901 tagtcgcatc agctaacttt ccgatttctt tacgattttc tccccctttt cttcaatttt
      961 actttgttag gatcgcattt ttaatgccaa cacataccag ttattggctg gacattaaac
     1021 aacttttaag tttaattact aactttatct aaagaggaga aatactagat gcgtaaaggc
     1081 gaagagctgt tcactggtgt cgtc
//
