LOCUS       sequence-82455-e         873 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Artificial transcription factor with no activator domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Senecal A, Munsky B, Proux F, Ly N, Braye FE, Zimmer C, Mueller F, 
            Darzacq X
  TITLE     Transcription Factors Modulate c-Fos Transcriptional Bursts.
  JOURNAL   Cell Rep. 2014 Jun 25. pii: S2211-1247(14)00447-1. doi: 
            10.1016/j.celrep.2014.05.053.
  PUBMED    24981864
REFERENCE   2  (bases 1 to 873)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep. 
            2014 Jun 25. pii: S2211-1247(14)00447-1. doi: 
            10.1016/j.celrep.2014.05.053."
COMMENT     Sequence Label: pAF1
FEATURES             Location/Qualifiers
     source          1..873
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    694..715
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(730..735,736..753,754..760)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    768..784
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     773..795
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     792..808
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     792..808
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     827..847
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        829..847
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 actggactag tggatccggg aggccgcccc agatcctggt acgcgggcgt tttaccctcc
       61 agctgagggg caaatgcagc gcatcgcgct gttcgggaac gcgcacctcg acagcctgct
      121 gtgcggaggg gccggtgaca gcacgatccg atcgggaccg tttacggctg cttgcccgcg
      181 tctgatctcc ctcgtgcatt gggctgggcg catcaaggtc acgctccagc gaatcggcga
      241 atgcatgcaa agacgcctga tccggtgttt gagctgaagt aggggacggt ttggcccttt
      301 tcatccctga tgcctggagg atacggtccc aacgctgcga ggctgggggg agcgttccac
      361 cgcgggcttc gagttcggtg acgcccactc tgcgaaagag ctgtaccaac ccgttcctgc
      421 tcatcccgaa ctgcgtcatg gcctcatcaa atgcgtacgc tgggtgggag tggcactgga
      481 aaaactccag cacgcgaacc acttgcgcgt agtcggcaac gcgatgggac gtgcgttcgc
      541 caatacggcg attgactctt ctgatcaatt ccggcgcgtg cggcaatccc tttttcactg
      601 catccatggc aggacgtccg ccgaggcagg ccaaggcgac gaggtggtcg ttggagacga
      661 ccggtggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc
      721 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
      781 acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgcaa ttaaccctca
      841 ctaaagggaa caaaagctgg aggacctcga ggg
//
