LOCUS       sequence-82457-e         967 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Artificial transcription factor with VP64.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 967)
  AUTHORS   Senecal A, Munsky B, Proux F, Ly N, Braye FE, Zimmer C, Mueller F, 
            Darzacq X
  TITLE     Transcription Factors Modulate c-Fos Transcriptional Bursts.
  JOURNAL   Cell Rep. 2014 Jun 25. pii: S2211-1247(14)00447-1. doi: 
            10.1016/j.celrep.2014.05.053.
  PUBMED    24981864
REFERENCE   2  (bases 1 to 967)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep. 
            2014 Jun 25. pii: S2211-1247(14)00447-1. doi: 
            10.1016/j.celrep.2014.05.053."
COMMENT     Sequence Label: pAF1-VP64
FEATURES             Location/Qualifiers
     source          1..967
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(12..161)
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     protein_bind    854..875
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(890..895,896..913,914..920)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    928..944
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     933..955
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 gattagttaa tcagcatgtc caggtcgaaa tcatcaaggg cgtcactgcc gagcatgtcg
       61 aggtcaaagt catcaagggc atccgaacca agcatgtcaa ggtcaaaatc atcgagggcg
      121 tcacttccca gcatatccag atcaaaatcg tccaatgcgt cagcccgtcc gggatccggg
      181 aggccgcccc agatcctggt acgcgggcgt tttaccctcc agctgagggg caaatgcagc
      241 gcatcgcgct gttcgggaac gcgcacctcg acagcctgct gtgcggaggg gccggtgaca
      301 gcacgatccg atcgggaccg tttacggctg cttgcccgcg tctgatctcc ctcgtgcatt
      361 gggctgggcg catcaaggtc acgctccagc gaatcggcga atgcatgcaa agacgcctga
      421 tccggtgttt gagctgaagt aggggacggt ttggcccttt tcatccctga tgcctggagg
      481 atacggtccc aacgctgcga ggctgggggg agcgttccac cgcgggcttc gagttcggtg
      541 acgcccactc tgcgaaagag ctgtaccaac ccgttcctgc tcatcccgaa ctgcgtcatg
      601 gcctcatcaa atgcgtacgc tgggtgggag tggcactgga aaaactccag cacgcgaacc
      661 acttgcgcgt agtcggcaac gcgatgggac gtgcgttcgc caatacggcg attgactctt
      721 ctgatcaatt ccggcgcgtg cggcaatccc tttttcactg catccatggc aggacgtccg
      781 ccgaggcagg ccaaggcgac gaggtggtcg ttggagacga ccggtggaaa gcgggcagtg
      841 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
      901 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
      961 gctatga
//
