LOCUS       sequence-82461-e         833 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses far-red fluorescent protein mNeptune2.5 in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Chu J, Haynes RD, Corbel SY, Li P, Gonzalez-Gonzalez E, Burg JS, 
            Ataie NJ, Lam AJ, Cranfill PJ, Baird MA, Davidson MW, Ng HL, Garcia 
            KC, Contag CH, Shen K, Blau HM, Lin MZ
  TITLE     Non-invasive intravital imaging of cellular differentiation with a 
            bright red-excitable fluorescent protein.
  JOURNAL   Nat Methods. 2014 Mar 16. doi: 10.1038/nmeth.2888.
  PUBMED    24633408
REFERENCE   2  (bases 1 to 833)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2014 Mar 16. doi: 10.1038/nmeth.2888."
COMMENT     Sequence Label: pcDNA3-mNeptune2.5
FEATURES             Location/Qualifiers
     source          1..833
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     47..66
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        47..65
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             98..832
                     /codon_start=1
                     /product="brighter version of the far-red fluorescent 
                     protein mNeptune2 with slightly blue-shifted spectra"
                     /label=mNeptune2.5
                     /translation="MVSKGEELIKENMHTKLYMEGTVNNHHFKCTHEGEGKPYEGTQTN
                     RIKVVEGGPLPFAFDILATCFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTVTQDTSLQDGCLIYNVKLRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRCD
                     MALKLVGGGHLHCNLKTTYRSKKPAKNLKMPGVYFVDRRLERIKEADNETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
ORIGIN
        1 ctctctggct aactagagaa cccactgctt actggcttat cgaaattaat acgactcact
       61 atagggagac ccaagcttgg taccgagctc ggatccgatg gtgagcaagg gcgaggagct
      121 gatcaaggag aacatgcaca cgaagctgta catggaaggc accgtgaaca accaccactt
      181 caagtgcacc cacgaagggg agggcaagcc ctacgagggc acccagacca acaggattaa
      241 ggtggtggag ggaggccccc tgccgttcgc attcgacatc ctggccacct gctttatgta
      301 cgggagcaag accttcatca accacaccca gggcatcccc gatttcttta agcagtcctt
      361 ccctgagggc ttcacatggg agagagtcac cacatacgaa gacgggggcg tgcttaccgt
      421 tacccaggac accagcctcc aggacggctg cttgatctac aacgtcaagc tcagaggggt
      481 gaacttccca tccaacggcc ctgtgatgca gaagaaaaca ctcggctggg aggccagtac
      541 cgagaccctg taccccgctg acggcggcct ggaaggcaga tgcgacatgg ccctgaagct
      601 cgtgggcggg ggccacctgc actgcaacct gaagaccaca tacagatcca agaaacccgc
      661 taagaacctc aagatgcccg gcgtctactt tgtggaccgc agactggaaa gaatcaagga
      721 ggccgacaat gagacctacg tcgagcagca cgaggtggct gtggccagat actgcgacct
      781 ccctagcaaa ctggggcaca aacttaatgg catggacgag ctgtacaagt aag
//
