LOCUS       sequence-8247-ex        5441 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5441)
  AUTHORS   Freeman KW, Gangula RD, Welm BE, Ozen M, Foster BA, Rosen JM, 
            Ittmann M, Greenberg NM, Spencer DM
  TITLE     Conditional activation of fibroblast growth factor receptor (FGFR) 
            1, but not FGFR2, in prostate cancer cells leads to increased 
            osteopontin induction, extracellular signal-regulated kinase 
            activation, and in vivo proliferation.
  JOURNAL   Cancer Res. 2003 Oct 1. 63(19):6237-43.
  PUBMED    14559809
REFERENCE   2  (bases 1 to 5441)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer Res.
            2003 Oct 1. 63(19):6237-43."
COMMENT     Sequence Label: pSH1/M-FGFR2-Fv-Fvls-E
FEATURES             Location/Qualifiers
     source          1..5441
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(66..83)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(237..825)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(317..336)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(996..1787,1788..1856))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1619..1638
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1857..1961)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2029..2047
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2109..2129)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        join(2114..2443,2444..2459,2460..2729)
                     /label=SR-alpha promoter
                     /note="hybrid promoter consisting of the SV40 early 
                     promoter plus part of the long terminal repeat of human 
                     T-lymphotrophic virus 1"
     rep_origin      2294..2429
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     LTR             2460..2729
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     intron          2774..2867
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     regulatory      2938..2947
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2944..2985
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSKPKDPSQR"
     CDS             4240..4560
                     /codon_start=1
                     /product="F36V mutant of FK506-binding protein FKBP12"
                     /label=FKBP-V
                     /note="binds synthetic ligands that do not bind wild-type 
                     FKBP"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             4567..4887
                     /codon_start=1
                     /product="F36V mutant of FK506-binding protein FKBP12"
                     /label=FKBP-V
                     /note="binds synthetic ligands that do not bind wild-type 
                     FKBP"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             4912..4938
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    4969..5103
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4993..5012)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5047..5066
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(5243..5265)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5406..5425
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
       61 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
      121 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
      181 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
      241 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
      301 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
      361 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
      421 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
      481 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
      541 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
      601 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
      661 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
      721 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
      781 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
      841 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
      901 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
      961 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     1021 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     1081 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     1141 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     1201 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     1261 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     1321 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     1381 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     1441 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     1501 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     1561 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     1621 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     1681 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     1741 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     1801 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     1861 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     1921 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     1981 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
     2041 acgaggccct ttcgtcttca aggtcgatcg acctcatgtt tgacagctta tcatcgataa
     2101 gcttctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag
     2161 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
     2221 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
     2281 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     2341 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     2401 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc tccgatcgag
     2461 gggctcgcat ctctccttca cgcgcccgcc gccctacctg aggccgccat ccaacgccgg
     2521 ttgagtcgcg ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag
     2581 gtaagtttaa agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct
     2641 agactcagcc ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt
     2701 tcgttttctg ttctgcgccg ttacagatcc aagctgcctc gatcgaggaa ctgaaaaacc
     2761 agaaagttaa ctggtaagtt tagtcttttt gtcttttatt tcaggtcccg gatccggtgg
     2821 tgcaaatcaa agaactgctc ctcagtggat gttgccttta cttctaggcc tgtacggagg
     2881 tgttacttct gctctaaaag ctgctctagc cctcgatcga gggctagagc ggccgcggcc
     2941 accatgggga gtagcaagag caagcctaag gaccccagcc agcgcctcga ggacttcagc
     3001 agccagccag ctgtgcacaa gctgaccaag cgcatccccc tgcggagaca ggtaacagtt
     3061 tcggccgagt ccagctcctc catgaactcc aacaccccgc tggtgaggat aacaacgcgt
     3121 ctgtcctcaa cagcggacac cccgatgcta gcaggggtct ccgagtatga gttgccagag
     3181 gatccaaagt gggaattccc cagagataag ctgacgctgg gcaaacccct gggggaaggt
     3241 tgcttcgggc aagtagtcat ggctgaagca gtgggaatcg ataaagacaa acccaaggag
     3301 gcggtcaccg tggcagtgaa gatgttgaaa gatgatgcca cagagaagga cctgtctgat
     3361 ctggtatcag agatggagat gatgaagatg attgggaaac ataagaacat tatcaacctc
     3421 ctgggggcct gcacgcagga tggacctctc tacgtcatag ttgaatatgc atcgaaaggc
     3481 aacctccggg aatacctccg agcccggagg ccacctggca tggagtactc ctatgacatt
     3541 aaccgtgtcc ccgaggagca gatgaccttc aaggacttgg tgtcctgcac ctaccagctg
     3601 gctagaggca tggagtactt ggcttcccaa aaatgtatcc atcgagattt ggctgccaga
     3661 aacgtgttgg taacagaaaa caatgtgatg aagatagcag actttggcct ggccagggat
     3721 atcaacaaca tagactacta taaaaagacc acaaatgggc gacttccagt caagtggatg
     3781 gctcctgaag ccctttttga tagagtttac actcatcaga gcgatgtctg gtccttcggg
     3841 gtgttaatgt gggagatctt tactttaggg ggctcaccct acccagggat tcccgtggag
     3901 gaacttttta agctgctcaa agagggacac aggatggaca agcccaccaa ctgcaccaat
     3961 gaactgtaca tgatgatgag ggattgctgg catgctgtac cctcacagag acccacattc
     4021 aagcagttgg tcgaagactt ggatcgaatt ctgactctca caaccaatga ggaatacttg
     4081 gatctcaccc agcctctcga acagtattct cctagttacc ccgacacaag tagctcttgt
     4141 tcttcagggg acgattctgt gttttctcca gaccccatgc cttatgaacc ctgtctgcct
     4201 cagtatccac acataaacgg cagtgttaaa acagtcgagg gcgtgcaggt ggaaaccatc
     4261 tccccaggag acgggcgcac cttccccaag cgcggccaga cctgcgtggt gcactacacc
     4321 gggatgcttg aagatggaaa gaaagttgat tcctcccggg acagaaacaa gccctttaag
     4381 tttatgctag gcaagcagga ggtgatccga ggctgggaag aaggggttgc ccagatgagt
     4441 gtgggtcaga gagccaaact gactatatct ccagattatg cctatggtgc cactgggcac
     4501 ccaggcatca tcccaccaca tgccactctc gtcttcgatg tggagcttct aaaactggaa
     4561 gtcgagggag tgcaggtgga aaccatctcc ccaggagacg ggcgcacctt ccccaagcgc
     4621 ggccagacct gcgtggtgca ctacaccggg atgcttgaag atggaaagaa agttgattcc
     4681 tcccgggaca gaaacaagcc ctttaagttt atgctaggca agcaggaggt gatccgaggc
     4741 tgggaagaag gggttgccca gatgagtgtg ggtcagagag ccaaactgac tatatctcca
     4801 gattatgcct atggtgccac tgggcaccca ggcatcatcc caccacatgc cactctcgtc
     4861 ttcgatgtgg agcttctaaa actggaatct ggcggtggat ccggagtcga ctatccgtac
     4921 gacgtaccag actacgcact cgactaagaa ttcctgattg attgaccgga tccagacatg
     4981 ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt
     5041 atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa
     5101 gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt
     5161 ttttaaagca agtaaaacct ctacaaatgt ggtatggctg attatgatcc tgcctcgcgc
     5221 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
     5281 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
     5341 ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtat actggcttaa
     5401 ctatgcggca tcagagcaga ttgtactgag agtgcaccat a
//
