LOCUS       sequence-82479-e        7934 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of rice codon-optimized Cas9 in plant cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7934)
  AUTHORS   Shan Q, Wang Y, Li J, Zhang Y, Chen K, Liang Z, Zhang K, Liu J, Xi 
            JJ, Qiu JL, Gao C
  TITLE     Targeted genome modification of crop plants using a CRISPR-Cas 
            system.
  JOURNAL   Nat Biotechnol. 2013 Aug;31(8):686-8. doi: 10.1038/nbt.2650.
  PUBMED    23929338
REFERENCE   2  (bases 1 to 7934)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt"; 
            journalName: "Nat Biotechnol"; date: "2013-08"; volume: "31"; issue:
            "8"; pages: "686-8"
COMMENT     Sequence Label: pJIT163-2NLSCas9
FEATURES             Location/Qualifiers
     source          1..7934
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        401..746
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     689..709
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             791..811
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             836..4939
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             836..4939
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             4940..4987
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     polyA_signal    5045..5221
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     primer_bind     complement(5764..5780)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5764..5780)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5777..5799)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5788..5804
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5812..5818,5819..5836,5837..5842))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5857..5878
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5995..6012)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6166..6754)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6246..6265)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6925..7716,7717..7785))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7548..7567
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7786..7890)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gagctcggta cccctactcc aaaaatgtca aagatacagt ctcagaagac caaagggcta
       61 ttgagacttt tcaacaaagg gtaatttcgg gaaacctcct cggattccat tgcccagcta
      121 tctgtcactt catcgaaagg acagtagaaa aggaaggtgg ctcctacaaa tgccatcatt
      181 gcgataaagg aaaggctatc attcaagatg cctctgccga cagtggtccc aaagatggac
      241 ccccacccac gaggagcatc gtggaaaaag aagacgttcc aaccacgtct tcaaagcaag
      301 tggattgatg tgacatctcc actgacgtaa gggatgacgc acaatcccac ccctactcca
      361 aaaatgtcaa agatacagtc tcagaagacc aaagggctat tgagactttt caacaaaggg
      421 taatttcggg aaacctcctc ggattccatt gcccagctat ctgtcacttc atcgaaagga
      481 cagtagaaaa ggaaggtggc tcctacaaat gccatcattg cgataaagga aaggctatca
      541 ttcaagatgc ctctgccgac agtggtccca aagatggacc cccacccacg aggagcatcg
      601 tggaaaaaga agacgttcca accacgtctt caaagcaagt ggattgatgt gacatctcca
      661 ctgacgtaag ggatgacgca caatcccact atccttcgca agacccttcc tctatataag
      721 gaagttcatt tcatttggag aggacagccc aagcttccac catggcgtgc aggtcgacgg
      781 atccatggcc cctaagaaga agagaaaggt cggtattcac ggcgttcctg cggcgatgga
      841 caagaagtat agtattggtc tggacattgg gacgaattcc gttggctggg ccgtgatcac
      901 cgatgagtac aaggtccctt ccaagaagtt taaggttctg gggaacaccg atcggcacag
      961 catcaagaag aatctcattg gagccctcct gttcgactca ggcgagaccg ccgaagcaac
     1021 aaggctcaag agaaccgcaa ggagacggta tacaagaagg aagaatagga tctgctacct
     1081 gcaggagatt ttcagcaacg aaatggcgaa ggtggacgat tcgttctttc atagattgga
     1141 ggagagtttc ctcgtcgagg aagataagaa gcacgagagg catcctatct ttggcaacat
     1201 tgtcgacgag gttgcctatc acgaaaagta ccccacaatc tatcatctgc ggaagaagct
     1261 tgtggactcg actgataagg cggaccttag attgatctac ctcgctctgg cacacatgat
     1321 taagttcagg ggccattttc tgatcgaggg ggatcttaac ccggacaata gcgatgtgga
     1381 caagttgttc atccagctcg tccaaaccta caatcagctc tttgaggaaa acccaattaa
     1441 tgcttcaggc gtcgacgcca aggcgatcct gtctgcacgc ctttcaaagt ctcgccggct
     1501 tgagaacttg atcgctcaac tcccgggcga aaagaagaac ggcttgttcg ggaatctcat
     1561 tgcactttcg ttggggctca caccaaactt caagagtaat tttgatctcg ctgaggacgc
     1621 aaagctgcag ctttccaagg acacttatga cgatgacctg gataaccttt tggcccaaat
     1681 cggcgatcag tacgcggact tgttcctcgc cgcgaagaat ttgtcggacg cgatcctcct
     1741 gagtgatatt ctccgcgtga acaccgagat tacaaaggcc ccgctctcgg cgagtatgat
     1801 caagcgctat gacgagcacc atcaggatct gacccttttg aaggctttgg tccggcagca
     1861 actcccagag aagtacaagg aaatcttctt tgatcaatcc aagaacggct acgctggtta
     1921 tattgacggc ggggcatcgc aggaggaatt ctacaagttt atcaagccaa ttctggagaa
     1981 gatggatggc acagaggaac tcctggtgaa gctcaatagg gaggaccttt tgcggaagca
     2041 aagaactttc gataacggca gcatccctca ccagattcat ctcggggagc tgcacgccat
     2101 cctgagaagg caggaagact tctacccctt tcttaaggat aaccgggaga agatcgaaaa
     2161 gattctgacg ttcagaattc cgtactatgt cggaccactc gcccggggta attccagatt
     2221 tgcgtggatg accagaaaga gcgaggaaac catcacacct tggaacttcg aggaagtggt
     2281 cgataagggc gcttccgcac agagcttcat tgagcgcatg acaaattttg acaagaacct
     2341 gcctaatgag aaggtccttc ccaagcattc cctcctgtac gagtatttca ctgtttataa
     2401 cgaactcacg aaggtgaagt atgtgaccga gggaatgcgc aagcccgcct tcctgagcgg
     2461 cgagcaaaag aaggcgatcg tggacctttt gtttaagacc aatcggaagg tcacagttaa
     2521 gcagctcaag gaggactact tcaagaagat tgaatgcttc gattccgttg agatcagcgg
     2581 cgtggaagac aggtttaacg cgtcactggg gacttaccac gatctcctga agatcattaa
     2641 ggataaggac ttcttggaca acgaggaaaa tgaggatatc ctcgaagaca ttgtcctgac
     2701 tcttacgttg tttgaggata gggaaatgat cgaggaacgc ttgaagacgt atgcccatct
     2761 cttcgatgac aaggttatga agcagctcaa gagaagaaga tacaccggat ggggaaggct
     2821 gtcccgcaag cttatcaatg gcattagaga caagcaatca gggaagacaa tccttgactt
     2881 tttgaagtct gatggcttcg cgaacaggaa ttttatgcag ctgattcacg atgactcact
     2941 tactttcaag gaggatatcc agaaggctca agtgtcggga caaggtgaca gtctgcacga
     3001 gcatatcgcc aaccttgcgg gatctcctgc aatcaagaag ggtattctgc agacagtcaa
     3061 ggttgtggat gagcttgtga aggtcatggg acggcataag cccgagaaca tcgttattga
     3121 gatggccaga gaaaatcaga ccacacaaaa gggtcagaag aactcgaggg agcgcatgaa
     3181 gcgcatcgag gaaggcatta aggagctggg gagtcagatc cttaaggagc acccggtgga
     3241 aaacacgcag ttgcaaaatg agaagctcta tctgtactat ctgcaaaatg gcagggatat
     3301 gtatgtggac caggagttgg atattaaccg cctctcggat tacgacgtcg atcatatcgt
     3361 tcctcagtcc ttccttaagg atgacagcat tgacaataag gttctcacca ggtccgacaa
     3421 gaaccgcggg aagtccgata atgtgcccag cgaggaagtc gttaagaaga tgaagaacta
     3481 ctggaggcaa cttttgaatg ccaagttgat cacacagagg aagtttgata acctcactaa
     3541 ggccgagcgc ggaggtctca gcgaactgga caaggcgggc ttcattaagc ggcaactggt
     3601 tgagactaga cagatcacga agcacgtggc gcagattctc gattcacgca tgaacacgaa
     3661 gtacgatgag aatgacaagc tgatccggga agtgaaggtc atcaccttga agtcaaagct
     3721 cgtttctgac ttcaggaagg atttccaatt ttataaggtg cgcgagatca acaattatca
     3781 ccatgctcat gacgcatacc tcaacgctgt ggtcggaaca gcattgatta agaagtaccc
     3841 gaagctcgag tccgaattcg tgtacggtga ctataaggtt tacgatgtgc gcaagatgat
     3901 cgccaagtca gagcaggaaa ttggcaaggc cactgcgaag tatttctttt actctaacat
     3961 tatgaatttc tttaagactg agatcacgct ggctaatggc gaaatccgga agagaccact
     4021 tattgagacc aacggcgaga caggggaaat cgtgtgggac aaggggaggg atttcgccac
     4081 agtccgcaag gttctctcta tgcctcaagt gaatattgtc aagaagactg aagtccagac
     4141 gggcgggttc tcaaaggaat ctattctgcc caagcggaac tcggataagc ttatcgccag
     4201 aaagaaggac tgggacccga agaagtatgg aggtttcgac tcaccaacgg tggcttactc
     4261 tgtcctggtt gtggcaaagg tggagaaggg aaagtcaaag aagctcaagt ctgtcaagga
     4321 gctcctgggt atcaccatta tggagaggtc cagcttcgaa aagaatccga tcgattttct
     4381 cgaggcgaag ggatataagg aagtgaagaa ggacctgatc attaagcttc caaagtacag
     4441 tcttttcgag ttggaaaacg gcaggaagcg catgttggct tccgcaggag agctccagaa
     4501 gggtaacgag cttgctttgc cgtccaagta tgtgaacttc ctctatctgg catcccacta
     4561 cgagaagctc aagggcagcc cagaggataa cgaacagaag caactgtttg tggagcaaca
     4621 caagcattat cttgacgaga tcattgaaca gatttcggag ttcagtaagc gcgtcatcct
     4681 cgccgacgcg aatttggata aggttctctc agcctacaac aagcaccggg acaagcctat
     4741 cagagagcag gcggaaaata tcattcatct cttcaccctg acaaaccttg gggctcccgc
     4801 tgcattcaag tattttgaca ctacgattga tcggaagaga tacacttcta cgaaggaggt
     4861 gctggatgca acccttatcc accaatcgat tactggcctc tacgagacgc ggatcgactt
     4921 gagtcagctc gggggggata agagaccagc ggcaaccaag aaggcaggac aagcgaagaa
     4981 gaagaagtag caattcggta cgctgaaatc accagtctct ctctacaaat ctatctctct
     5041 ctattttctc cataaataat gtgtgagtag tttcccgata agggaaatta gggttcttat
     5101 agggtttcgc tcatgtgttg agcatataag aaacccttag tatgtatttg tatttgtaaa
     5161 atacttctat caataaaatt tctaattcct aaaaccaaaa tccagtacta aaatccagat
     5221 ctcctaaagt ccctatagat ctttgtcgtg aatataaacc agacacgaga cgactaaacc
     5281 tggagcccag acgccgttcg aagctagaag taccgcttag gcaggaggcc gttagggaaa
     5341 agatgctaag gcagggttgg ttacgttgac tcccccgtag gtttggttta aatatgatga
     5401 agtggacgga aggaaggagg aagacaagga aggataaggt tgcaggccct gtgcaaggta
     5461 agaagatgga aatttgatag aggtacgcta ctatacttat actatacgct aagggaatgc
     5521 ttgtatttat accctatacc ccctaataac cccttatcaa tttaagaaat aatccgcata
     5581 agcccccgct taaaaattgg tatcagagcc atgaataggt ctatgaccaa aactcaagag
     5641 gataaaacct caccaaaata cgaaagagtt cttaactcta aagataaaag atctttcaag
     5701 atcaaaacta gttccctcac accggagcat gcgatatcct cgagagatct aggcgtaatc
     5761 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     5821 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     5881 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     5941 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     6001 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     6061 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     6121 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     6181 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     6241 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     6301 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     6361 atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     6421 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     6481 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     6541 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     6601 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     6661 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     6721 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     6781 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     6841 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     6901 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     6961 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     7021 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     7081 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     7141 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     7201 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     7261 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     7321 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     7381 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     7441 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     7501 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     7561 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     7621 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     7681 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     7741 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     7801 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     7861 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgccag
     7921 tgccaagcta attc
//
