LOCUS       sequence-8248-ex        5426 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5426)
  AUTHORS   Freeman KW, Gangula RD, Welm BE, Ozen M, Foster BA, Rosen JM, 
            Ittmann M, Greenberg NM, Spencer DM
  TITLE     Conditional activation of fibroblast growth factor receptor (FGFR) 
            1, but not FGFR2, in prostate cancer cells leads to increased 
            osteopontin induction, extracellular signal-regulated kinase 
            activation, and in vivo proliferation.
  JOURNAL   Cancer Res. 2003 Oct 1. 63(19):6237-43.
  PUBMED    14559809
REFERENCE   2  (bases 1 to 5426)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer Res.
            2003 Oct 1. 63(19):6237-43."
COMMENT     Sequence Label: pSH1/M-FGFR3-Fv-Fvls-E
FEATURES             Location/Qualifiers
     source          1..5426
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(66..83)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(237..825)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(317..336)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(996..1787,1788..1856))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1619..1638
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1857..1961)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2029..2047
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2109..2129)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        join(2114..2443,2444..2459,2460..2729)
                     /label=SR-alpha promoter
                     /note="hybrid promoter consisting of the SV40 early 
                     promoter plus part of the long terminal repeat of human 
                     T-lymphotrophic virus 1"
     rep_origin      2294..2429
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     LTR             2460..2729
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     intron          2774..2867
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     regulatory      2938..2947
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2944..2985
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSKPKDPSQR"
     CDS             4225..4545
                     /codon_start=1
                     /product="F36V mutant of FK506-binding protein FKBP12"
                     /label=FKBP-V
                     /note="binds synthetic ligands that do not bind wild-type 
                     FKBP"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             4552..4872
                     /codon_start=1
                     /product="F36V mutant of FK506-binding protein FKBP12"
                     /label=FKBP-V
                     /note="binds synthetic ligands that do not bind wild-type 
                     FKBP"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             4897..4923
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    4954..5088
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4978..4997)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5032..5051
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(5228..5250)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5391..5410
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
       61 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
      121 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
      181 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
      241 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
      301 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
      361 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
      421 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
      481 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
      541 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
      601 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
      661 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
      721 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
      781 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
      841 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
      901 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
      961 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     1021 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     1081 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     1141 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     1201 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     1261 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     1321 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     1381 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     1441 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     1501 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     1561 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     1621 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     1681 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     1741 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     1801 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     1861 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     1921 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     1981 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
     2041 acgaggccct ttcgtcttca aggtcgatcg acctcatgtt tgacagctta tcatcgataa
     2101 gcttctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag
     2161 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
     2221 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
     2281 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     2341 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     2401 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc tccgatcgag
     2461 gggctcgcat ctctccttca cgcgcccgcc gccctacctg aggccgccat ccaacgccgg
     2521 ttgagtcgcg ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag
     2581 gtaagtttaa agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct
     2641 agactcagcc ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt
     2701 tcgttttctg ttctgcgccg ttacagatcc aagctgcctc gatcgaggaa ctgaaaaacc
     2761 agaaagttaa ctggtaagtt tagtcttttt gtcttttatt tcaggtcccg gatccggtgg
     2821 tgcaaatcaa agaactgctc ctcagtggat gttgccttta cttctaggcc tgtacggagg
     2881 tgttacttct gctctaaaag ctgctctagc cctcgatcga gggctagagc ggccgcggcc
     2941 accatgggga gtagcaagag caagcctaag gaccccagcc agcgcctcga cctgcgcagt
     3001 cccccaaaga agggcttggg ctcgcccacc gtgcacaagg tctctcgctt cccgcttaag
     3061 cgacaggtgt ccttggaatc taactcctct atgaactcca acacacccct tgtccggatt
     3121 gcccggctgt cctcaggaga aggtcctgtt ctggccaatg tttctgaact tgagctgcct
     3181 gctgacccca agtgggagct atccaggacc cggctgacac ttggtaagcc tcttggagaa
     3241 ggctgctttg gacaggtggt catggcagaa gctattggca tcgacaagga ccgtactgcc
     3301 aagcctgtca ccgtggccgt gaagatgctg aaagatgatg cgactgacaa ggacctgtcg
     3361 gacctggtat ctgagatgga gatgatgaaa atgattggca agcacaagaa catcattaac
     3421 ctgctggggg cgtgcacaca gggtgggccc ctgtatgtgc tggtggagta cgcagccaag
     3481 ggcaatctcc gggagttcct tcgggcgcgg cggcctccag gcatggacta ctcctttgat
     3541 gcctgcaggc tgccagagga acagctcacc tgcaaggatc tagtgtcctg tgcctaccag
     3601 gtggcacggg gcatggaata cttggcttct cagaagtgta ttcacagaga cttggctgcc
     3661 agaaacgtcc tggtgaccga ggacaatgtg atgaagattg cggactttgg cctggctcga
     3721 gatgtgcaca acctggacta ctacaagaag accacaaatg gccggctacc tgtgaagtgg
     3781 atggcaccag aggccctttt tgaccgagtc tacacccacc agagtgatgt ttggtctttt
     3841 ggtgtcctcc tctgggagat ctttacgctg gggggctcac cgtatcctgg catcccagtg
     3901 gaagagcttt tcaagctgtt gaaagagggc caccgcatgg acaagccagc cagctgcaca
     3961 catgacctgt acatgatcat gcgggaatgt tggcatgcgg tgccttcaca gaggcccacc
     4021 ttcaagcagt tggtagagga tttagaccgc atcctcactg tgacatcaac cgacgagtac
     4081 ttggacctct ccgtgccgtt tgagcagtac tcgccaggtg gccaggacac gcctagctcc
     4141 agctcgtccg gagatgactc ggtgttcacc catgacctgc tacccccagg tccacccagt
     4201 aacgggggac ctcggacggt cgagggcgtg caggtggaaa ccatctcccc aggagacggg
     4261 cgcaccttcc ccaagcgcgg ccagacctgc gtggtgcact acaccgggat gcttgaagat
     4321 ggaaagaaag ttgattcctc ccgggacaga aacaagccct ttaagtttat gctaggcaag
     4381 caggaggtga tccgaggctg ggaagaaggg gttgcccaga tgagtgtggg tcagagagcc
     4441 aaactgacta tatctccaga ttatgcctat ggtgccactg ggcacccagg catcatccca
     4501 ccacatgcca ctctcgtctt cgatgtggag cttctaaaac tggaagtcga gggagtgcag
     4561 gtggaaacca tctccccagg agacgggcgc accttcccca agcgcggcca gacctgcgtg
     4621 gtgcactaca ccgggatgct tgaagatgga aagaaagttg attcctcccg ggacagaaac
     4681 aagcccttta agtttatgct aggcaagcag gaggtgatcc gaggctggga agaaggggtt
     4741 gcccagatga gtgtgggtca gagagccaaa ctgactatat ctccagatta tgcctatggt
     4801 gccactgggc acccaggcat catcccacca catgccactc tcgtcttcga tgtggagctt
     4861 ctaaaactgg aatctggcgg tggatccgga gtcgactatc cgtacgacgt accagactac
     4921 gcactcgact aagaattcct gattgattga ccggatccag acatgataag atacattgat
     4981 gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt
     5041 gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat
     5101 tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa
     5161 aacctctaca aatgtggtat ggctgattat gatcctgcct cgcgcgtttc ggtgatgacg
     5221 gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg
     5281 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag
     5341 ccatgaccca gtcacgtagc gatagcggag tgtatactgg cttaactatg cggcatcaga
     5401 gcagattgta ctgagagtgc accata
//
