LOCUS       sequence-82488-e         892 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of human HMG-CoA lyase (HL).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 892)
  AUTHORS   Montgomery C, Miziorko HM
  TITLE     Influence of multiple cysteines on human 
            3-hydroxy-3-methylglutaryl-CoA lyase activity and formation of 
            inter-subunit adducts.
  JOURNAL   Arch Biochem Biophys. 2011 Jul;511(1-2):48-55. doi: 
            10.1016/j.abb.2011.04.004. Epub 2011 Apr 13.
  PUBMED    21514269
REFERENCE   2  (bases 1 to 892)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Arch 
            Biochem Biophys."; date: "2011-07"; volume: "511(1-2):48-55. doi"; 
            pages: " 10.1016/j.abb.2011.04.004. Epub 2011 Apr 13"
COMMENT     Sequence Label: Human HMG-CoA lyase (HL)
FEATURES             Location/Qualifiers
     source          1..892
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     47..66
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        47..65
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    66..90
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             105..127
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             138..155
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             165..182
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             189..233
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             249..263
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
ORIGIN
        1 atgcgtccgg cgtagaggat cgagatcgat ctcgatcccg cgaaattaat acgactcact
       61 ataggggaat tgtgagcgga taacaattcc cctctagaaa taattttgtt taactttaag
      121 aaggagatat acatatgcac catcatcatc atcattcttc tggtctggtg ccacgcggtt
      181 ctggtatgaa agaaaccgct gctgctaaat tcgaacgcca gcacatggac agcccagatc
      241 tgggtaccga cgacgacgac aaggccatgg gcactttacc aaagcgggtg aaaattgtgg
      301 aagttggtcc ccgagatgga ctacaaaatg aaaagaatat cgtatctact ccagtgaaaa
      361 tcaagctgat agacatgctt tctgaagcag gactctctgt tatagaaacc accagctttg
      421 tgtctcctaa gtgggttccc cagatgggtg accacactga agtcttgaag ggcattcaga
      481 agtttcctgg catcaactac ccagtcctga ccccaaattt gaaaggcttc gaggcagcgg
      541 ttgctgctgg agccaaggaa gtagtcatct ttggagctgc ctcagagctc ttcaccaaga
      601 agaacatcaa ttgttccata gaggagagtt ttcagaggtt tgacgcaatc ctgaaggcag
      661 cgcagtcagc caatatttct gtgcgggggt acgtctcctg tgctcttggc tgcccttatg
      721 aagggaagat ctccccagct aaagtagctg aggtcaccaa gaagttctac tcaatgggct
      781 gctacgagat ctccctgggg gacaccattg gtgtgggcac cccagggatc atgaaagaca
      841 tgctatctgc tgtcatgcag gaagtgcctc tggctgccct ggctgtccac tg
//
