LOCUS       sequence-82489-e         849 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of human Mevalonate Kinase (MK).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 849)
  AUTHORS   Potter D, Miziorko HM
  TITLE     Identification of catalytic residues in human mevalonate kinase.
  JOURNAL   J Biol Chem. 1997 Oct 10;272(41):25449-54.
  PUBMED    9325256
REFERENCE   2  (bases 1 to 849)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "1997-10-10"; volume: "272(41)"; pages: "25449-54"
COMMENT     Sequence Label: Human Mevalonate Kinase (MK)
FEATURES             Location/Qualifiers
     source          1..849
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     42..61
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        42..60
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             92..114
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa ttaatacgac tcactatagg
       61 gagaccacaa cggtttccct ctagaaataa ttttgtttaa ctttaagaag gagatatacc
      121 atggtgtcag aagtcctact ggtgtctgct ccggggaaag tcatccttca tggagaacat
      181 gccgtggtac atggcaaggt agcactggct gtatccttga acttgagaac attcctccgg
      241 cttcaacccc acagcaatgg gaaagtggac ctcagcttac ccaacattgg tatcaagcgg
      301 gcctgggatg tggccaggct tcagtcactg gacacaagct ttctggagca aggtgatgtc
      361 acaacaccca cctcagagca agtggagaag ctaaaggagg ttgcaggctt gcctgacgac
      421 tgtgctgtca ccgagcgcct ggctgtgctg gcctttcttt acttatacct gtccatctgc
      481 cggaagcaga gggccctgcc gagcctggat atcgtagtgt ggtcggagct gccccccggg
      541 gcgggcttgg gctccagcgc cgcctactcg gtgtgtctgg cagcagccct cctgactgtg
      601 tgcgaggaga tcccaaaccc gctgaaggac ggggattgcg tcaacaggtg gaccaaggag
      661 gatttggagc taattaacaa gtgggccttc caaggggaga gaatgattca cgggaacccc
      721 tccggagtgg acaatgctgt cagcacctgg gggaggagcc ctccgatacc atcaagggaa
      781 gatttcatcc ttaaagaggt cgccagctct ccagatcctg ctgaccaaca cccaaagtcc
      841 ctcgcaata
//
