LOCUS       sequence-82499-e         870 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial nuclease-null TD Cas9 expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Esvelt KM, Mali P, Braff JL, Moosburner M, Yaung SJ, Church GM
  TITLE     Orthogonal Cas9 proteins for RNA-guided gene regulation and editing.
  JOURNAL   Nat Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681.
  PUBMED    24076762
REFERENCE   2  (bases 1 to 870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681."
COMMENT     Sequence Label: DS-TDcasN-
FEATURES             Location/Qualifiers
     source          1..870
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gctgtacctg tactacaccc agctgggcaa gtgcatgtac tgcggcaagc ccatcgagat
       61 cggccacgtg ttcgacacca gcaactacga catcgctgct atctaccccc agagcaagat
      121 caaggacgac agcatcagca accgcgtgct ggtgtgcagc agctgcgcca agaacaagga
      181 ggacaagtac cctctgaaga gcgagatcca gagcaagcag cgcggcttct ggaacttcct
      241 gcagcgcaac aacttcatca gcctggagaa gctgaaccgc ctgacccgcg ccacccccat
      301 cagcgacgac gagaccgcca agttcatcgc ccgccagctg gtggagactc gccaagctac
      361 caaggtggcc gccaaggtgc tggagaagat gttccccgag accaagatcg tgtacagcaa
      421 ggccgagacc gtgagcatgt tccgcaacaa gttcgacatc gtgaagtgcc gcgagatcaa
      481 cgacttccac cacgcccacg acgcctacct gaacatcgtg gtgggcaacg tgtacaacac
      541 caagttcacc aacaacccct ggaatttcat taaggagaag cgcgacaacc ccaagatcgc
      601 cgacacctac aactactaca aggtgttcga ctacgacgtg aagcgcaaca acatcaccgc
      661 ctgggagaag ggcaagacca tcatcaccgt gaaggacatg ctgaagcgca acacccccat
      721 ctacacccgc caggccgcct gcaagaaggg cgagctgttc aaccagacca tcatgaagaa
      781 gggcctgggc cagcaccccc tgaagaagga gggccccttc agcaacatca gcaagtacgg
      841 cggctacaac aaggtgagcg ccgcctacta
//
