LOCUS       sequence-82500-e         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses truncated version of NLL-MetRS from endogenous MetG 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Mahdavi A, Szychowski J, Ngo JT, Sweredoski MJ, Graham RL, Hess S, 
            Schneewind O, Mazmanian SK, Tirrell DA
  TITLE     Identification of secreted bacterial proteins by noncanonical amino 
            acid tagging.
  JOURNAL   Proc Natl Acad Sci U S A. 2014 Jan 7;111(1):433-8. doi: 
            10.1073/pnas.1301740111. Epub 2013 Dec 17.
  PUBMED    24347637
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1301740111"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2014-01-7- 7"; volume: "111"; issue: "1"; pages: "433-8"
COMMENT     Sequence Label: pAM1
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             8..25
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      89..183
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
ORIGIN
        1 aggatcgcat caccatcacc atcacggatc cgcatgcgag ctcggtaccc cgggtcgacc
       61 tgcagccaag cttaattagc tgagcttgga ctcctgttga tagatccagt aatgacctca
      121 gaactccatc tggatttgtt cagaacgctc ggttgccgcc gggcgttttt tattggtgag
      181 aatccaagct agctctagag acgtccggcc ggagctccac cgcggtggcg gccgctctag
      241 agtcacttac ttaacatttt cccatttggt actatctaac cccttttcac tattaagaag
      301 taatgcctac tatgactcaa gtcgcgaaga aaattctggt gacgtgcgca aacccgtacg
      361 ctaacggctc aatccacctc ggccatatgc tggagcacat ccaggctgat gtctgggtcc
      421 gttaccagcg aatgcgcggc cacgaggtca acttcatctg cgccgacgat gcccacggta
      481 caccgatcat gctgaaagct cagcagcttg gtatcacccc ggagcagatg attggcgaaa
      541 tgagtcagga gcatcagact gatttcgcag gctttaacat cagctatgac aactatcact
      601 cgacgcacag cgaagagaac cgccagttgt cagaacttat ctactctcgc ctgaaagaaa
      661 acggttttat taaaaaccgc accatctctc agctgtacga tccggaaaaa ggcatgttcc
      721 tgccggaccg ttttgtgaaa ggcacctgcc cgaaatgtaa atccccggat caatacggcg
      781 ataactgcga agtctgcggc gcgacctaca gcccgactga actgatcgag
//
