LOCUS       sequence-82514-e        6497 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses alpha subunit of hERG1a K channel in Xenopus oocytes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6497)
  AUTHORS   Mitcheson JS, Chen J, Lin M, Culberson C, Sanguinetti MC
  TITLE     A structural basis for drug-induced long QT syndrome.
  JOURNAL   Proc Natl Acad Sci U S A. 2000 Oct 24;97(22):12329-33.
  PUBMED    11005845
REFERENCE   2  (bases 1 to 6497)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2000-10-24"; volume: "97(22)"; pages: 
            "12329-33"
COMMENT     Sequence Label: pSP64-hERG1a
FEATURES             Location/Qualifiers
     source          1..6497
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(3559..3575)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3559..3575)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3572..3594)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3583..3599
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3607..3613,3614..3631,3632..3637))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3652..3673
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3790..3807)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3961..4549)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4041..4060)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4720..5511,5512..5580))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5343..5362
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5581..5685)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5753..5771
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(5809..5831)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5931..5950
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6165..6184
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        6475..6493
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     6475..6492
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 aagcttctca ggatgccggt gcggaggggc cacgtcgcgc cgcagaacac cttcctggac
       61 accatcatcc gcaagtttga gggccagagc cgtaagttca tcatcgccaa cgctcgggtg
      121 gagaactgcg ccgtcatcta ctgcaacgac ggcttctgcg agctgtgcgg ctactcgcgg
      181 gccgaggtga tgcagcgacc ctgcacctgc gacttcctgc acgggccgcg cacgcagcgc
      241 cgcgctgccg cgcagatcgc gcaggcactg ctgggcgccg aggagcgcaa agtggaaatc
      301 gccttctacc ggaaagatgg gagctgcttc ctatgtctgg tggatgtggt gcccgtgaag
      361 aacgaggatg gggctgtcat catgttcatc ctcaatttcg aggtggtgat ggagaaggac
      421 atggtggggt ccccggctca tgacaccaac caccggggcc cccccaccag ctggctggcc
      481 ccaggccgcg ccaagacctt ccgcctgaag ctgcccgcgc tgctggcgct gacggcccgg
      541 gagtcgtcgg tgcggtcggg cggcgcgggc ggcgcgggcg ccccgggggc cgtggtggtg
      601 gacgtggacc tgacgcccgc ggcacccagc agcgagtcgc tggccctgga cgaagtgaca
      661 gccatggaca accacgtggc agggctcggg cccgcggagg agcggcgtgc gctggtgggt
      721 cccggctctc cgccccgcag cgcgcccggc cagctcccat cgccccgggc gcacagcctc
      781 aaccccgacg cctcgggctc cagctgcagc ctggcccgga cgcgctcccg agaaagctgc
      841 gccagcgtgc gccgcgcctc gtcggccgac gacatcgagg ccatgcgcgc cggggtgctg
      901 cccccgccac cgcgccacgc cagcaccggg gccatgcacc cactgcgcag cggcttgctc
      961 aactccacct cggactccga cctcgtgcgc taccgcacca ttagcaagat tccccaaatc
     1021 accctcaact ttgtggacct caagggcgac cccttcttgg cttcgcccac cagtgaccgt
     1081 gagatcatag cacctaagat aaaggagcga acccacaatg tcactgagaa ggtcacccag
     1141 gtcctgtccc tgggcgccga cgtgctgcct gagtacaagc tgcaggcacc gcgcatccac
     1201 cgctggacca tcctgcatta cagccccttc aaggccgtgt gggactggct catcctgctg
     1261 ctggtcatct acacggctgt cttcacaccc tactcggctg ccttcctgct gaaggagacg
     1321 gaagaaggcc cgcctgctac cgagtgtggc tacgcgtgcc agccgctggc tgtggtggac
     1381 ctcatcgtgg acatcatgtt cattgtggac atcctcatca acttccgcac cacctacgtc
     1441 aatgccaacg aggaggtggt cagccacccc ggccgcatcg ccgtccacta cttcaagggc
     1501 tggttcctca tcgacatggt ggccgccatc cccttcgacc tgctcatctt cggctctggc
     1561 tctgaggagc tgatcgggct gctgaagact gcgcggctgc tgcggctggt gcgcgtggcg
     1621 cggaagctgg atcgctactc agagtacggc gcggccgtgc tgttcttgct catgtgcacc
     1681 tttgcgctca tcgcgcactg gctagcctgc atctggtacg ccatcggcaa catggagcag
     1741 ccacacatgg actcacgcat cggctggctg cacaacctgg gcgaccagat aggcaaaccc
     1801 tacaacagca gcggcctggg cggcccctcc atcaaggaca agtatgtgac ggcgctctac
     1861 ttcaccttca gcagcctcac cagtgtgggc ttcggcaacg tctctcccaa caccaactca
     1921 gagaagatct tctccatctg cgtcatgctc attggctccc tcatgtatgc tagcatcttc
     1981 ggcaacgtgt cggccatcat ccagcggctg tactcgggca cagcccgcta ccacacacag
     2041 atgctgcggg tgcgggagtt catccgcttc caccagatcc ccaatcccct gcgccagcgc
     2101 ctcgaggagt acttccagca cgcctggtcc tacaccaacg gcatcgacat gaacgcggtg
     2161 ctgaagggct tccctgagtg cctgcaggct gacatctgcc tgcacctgaa ccgctcactg
     2221 ctgcagcact gcaaaccctt ccgaggggcc accaagggct gccttcgggc cctggccatg
     2281 aagttcaaga ccacacatgc accgccaggg gacacactgg tgcatgctgg ggacctgctc
     2341 accgccctgt acttcatctc ccggggctcc atcgagatcc tgcggggcga cgtcgtcgtg
     2401 gccatcctgg ggaagaatga catctttggg gagcctctga acctgtatgc aaggcctggc
     2461 aagtcgaacg gggatgtgcg ggccctcacc tactgtgacc tacacaagat ccatcgggac
     2521 gacctgctgg aggtgctgga catgtaccct gagttctccg accacttctg gtccagcctg
     2581 gagatcacct tcaacctgcg agataccaac atgatcccgg gctcccccgg cagtacggag
     2641 ttagagggtg gcttcagtcg gcaacgcaag cgcaagttgt ccttccgcag gcgcacggac
     2701 aaggacacgg agcagccagg ggaggtgtcg gccttggggc cgggccgggc gggggcaggg
     2761 ccgagtagcc ggggccggcc gggggggccg tggggggaga gcccgtccag tggcccctcc
     2821 agccctgaga gcagtgagga tgagggccca ggccgcagct ccagccccct ccgcctggtg
     2881 cccttctcca gccccaggcc ccccggagag ccgccgggtg gggagcccct gatggaggac
     2941 tgcgagaaga gcagcgacac ttgcaacccc ctgtcaggcg ccttctcagg agtgtccaac
     3001 attttcagct tctgggggga cagtcggggc cgccagtacc aggagctccc tcgatgcccc
     3061 gcccccaccc ccagcctcct caacatcccc ctctccagcc cgggtcggcg gccccggggc
     3121 gacgtggaga gcaggctgga tgccctccag cgccagctca acaggctgga gacccggctg
     3181 agtgcagaca tggccactgt cctgcagctg ctacagaggc agatgacgct ggtcccgccc
     3241 gcctacagtg ctgtgaccac cccggggcct ggccccactt ccacatcccc gctgttgccc
     3301 gtcagccccc tccccaccct caccttggac tcgctttctc aggtttccca gttcatggcg
     3361 tgtgaggagc tgcccccggg ggccccagag cttccccaag aaggccccac acgacgcctc
     3421 tccctaccgg gccagctggg ggccctcacc tcccagcccc tgcacagaca cggctcggac
     3481 ccgggcagtt agtggggctg cccagtgtgg acacgtggct cacccggatc cccgggcgag
     3541 ctcgaattcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     3601 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     3661 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     3721 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     3781 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     3841 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     3901 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     3961 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     4021 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     4081 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     4141 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     4201 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     4261 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     4321 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     4381 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
     4441 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     4501 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     4561 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     4621 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     4681 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     4741 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     4801 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     4861 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     4921 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     4981 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     5041 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     5101 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     5161 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     5221 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     5281 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     5341 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     5401 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     5461 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     5521 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     5581 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     5641 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     5701 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
     5761 tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat
     5821 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg
     5881 tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga
     5941 gcagattgta ctgagagtgc accatatcga cgctctccct tatgcgactc ctgcattagg
     6001 aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc
     6061 aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa
     6121 caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata
     6181 taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag
     6241 aggatctggc tagcgatgac cctgctgatt ggttcgctga ccatttccgg ggtgcggaac
     6301 ggcgttacca gaaactcaga aggttcgtcc aaccaaaccg actctgacgg cagtttacga
     6361 gagagatgat agggtctgct tcagtaagcc agatgctaca caattaggct tgtacatatt
     6421 gtcgttagaa cgcggctaca attaatacat aaccttatgt atcatacaca tacgatttag
     6481 gtgacactat agaatac
//
