LOCUS       sequence-82529-e        5282 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses alpha subunit for hKCNQ1 channel in Xenopus oocytes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5282)
  AUTHORS   Sanguinetti MC, Curran ME, Zou A, Shen J, Spector PS, Atkinson DL, 
            Keating MT
  TITLE     Coassembly of K(V)LQT1 and minK (IsK) proteins to form cardiac I(Ks)
            potassium channel.
  JOURNAL   Nature. 1996 Nov 7;384(6604):80-3.
  PUBMED    8900283
REFERENCE   2  (bases 1 to 5282)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature."; 
            date: "1996-11-7"; volume: "384(6604)"; pages: "80-3"
COMMENT     Sequence Label: pSP64-hKCNQ1
FEATURES             Location/Qualifiers
     source          1..5282
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..21
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     3..20
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(2370..2386)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2370..2386)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2383..2405)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2394..2410
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2418..2424,2425..2442,2443..2448))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2463..2484
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2601..2618)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2772..3360)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2852..2871)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3531..4322,4323..4391))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4154..4173
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4392..4496)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4564..4582
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(4620..4642)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4742..4761
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4976..4995
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 cgatttaggt gacactatag aatacaagct tctcaggatg gccgcggcct cctccccgcc
       61 cagggccgag aggaagcgct ggggttgggg ccgcctgcca ggcgcccggc ggggcagcgc
      121 gggcctggcc aagaagtgcc ccttctcgct ggagctggcg gagggcggcc cggcgggcgg
      181 cgcgctctac gcgcccatcg cgcccggcgc cccaggtccc gcgccccctg cgtccccggc
      241 cgcgcccgcc gcgcccccag ttgcctccga ccttggcccg cggccgccgg tgagcctaga
      301 cccgcgcgtc tccatctaca gcacgcgccg cccggtgttg gcgcgcaccc acgtccaggg
      361 ccgcgtctac aacttcctcg agcgtcccac cggctggaaa tgcttcgttt accacttcgc
      421 cgtcttcctc atcgtcctgg tctgcctcat cttcagcgtg ctgtccacca tcgagcagta
      481 tgccgccctg gccacgggga ctctcttctg gatggagatc gtgctggtgg tgttcttcgg
      541 gacggagtac gtggtccgcc tctggtccgc cggctgccgc agcaagtacg tgggcctctg
      601 ggggcggctg cgctttgccc ggaagcccat ttccatcatc gacctcatcg tggtcgtggc
      661 ctccatggtg gtcctctgcg tgggctccaa ggggcaggtg tttgccacgt cggccatcag
      721 gggcatccgc ttcctgcaga tcctgaggat gctacacgtc gaccgccagg gaggcacctg
      781 gaggctcctg ggctccgtgg tcttcatcca ccgccaggag ctgataacca ccctgtacat
      841 cggcttcctg ggcctcatct tctcctcgta ctttgtgtac ctggctgaga aggacgcggt
      901 gaacgagtca ggccgcgtgg agttcggcag ctacgcagat gcgctgtggt ggggggtggt
      961 cacagtcacc accatcggct atggggacaa ggtgccccag acgtgggtcg ggaagaccat
     1021 cgcctcctgc ttctctgtct ttgccatctc cttctttgcg ctcccagcgg ggattcttgg
     1081 ctcggggttt gccctgaagg tgcagcagaa gcagaggcag aagcacttca accggcagat
     1141 cccggcggcg gcctcactca ttcagaccgc atggaggtgc tatgctgccg agaaccccga
     1201 ctcctccacc tggaagatct acatccggaa ggccccccgg agccacactc tgctgtcacc
     1261 cagccccaaa cccaagaagt ctgtggtggt aaagaaaaaa aagttcaagc tggacaaaga
     1321 caatggggtg actcctggag agaagatgct cacagtcccc catatcacgt gcgacccccc
     1381 agaagagcgg cggctggacc acttctctgt cgacggctat gacagttctg taaggaagag
     1441 cccaacactg ctggaagtga gcatgcccca tttcatgaga accaacagct tcgccgagga
     1501 cctggacctg gaaggggaga ctctgctgac acccatcacc cacatctcac agctgcggga
     1561 acaccatcgg gccaccatta aggtcattcg acgcatgcag tactttgtgg ccaagaagaa
     1621 attccagcaa gcgcggaagc cttacgatgt gcgggacgtc attgagcagt actcgcaggg
     1681 ccacctcaac ctcatggtgc gcatcaagga gctgcagagg aggctggacc agtccattgg
     1741 gaagccctca ctgttcatct ccgtctcaga aaagagcaag gatcgcggca gcaacacgat
     1801 cggcgcccgc ctgaaccgag tagaagacaa ggtgacgcag ctggaccaga ggctggcact
     1861 catcaccgac atgcttcacc agctgctctc cttgcacggt ggcagcaccc ccggcagcgg
     1921 cggccccccc agagagggcg gggcccacat cacccagccc tgcggcagtg gcggctccgt
     1981 cgaccctgag ctcttcctgc ccagcaacac cctgcccacc tacgagcagc tgaccgtgcc
     2041 caggaggggc cccgatgagg ggtcctgagg aggggatggg gctgggggat gggcctgagt
     2101 gagaggggag gccaagagtg gccccacctg gccctctctg aaggaggcca cctcctaaaa
     2161 ggcccagaga gaagagcccc actctcagag gccccaatac cccatggacc atgctgtctg
     2221 gcacagcctg cacttggggg ctcagcaagg ccacctcttc ctggccggtg tgggggcccc
     2281 gtctcaggtc tgagttgtta ccggatcccc gggcgagctc ccaaaaaaaa aaaaaaaaaa
     2341 aaaaaaaaaa aaccgaattc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     2401 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     2461 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     2521 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     2581 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     2641 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     2701 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     2761 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     2821 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     2881 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     2941 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     3001 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     3061 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     3121 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     3181 aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg
     3241 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     3301 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     3361 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     3421 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     3481 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     3541 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     3601 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     3661 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     3721 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     3781 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     3841 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     3901 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     3961 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     4021 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     4081 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     4141 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     4201 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     4261 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     4321 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     4381 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     4441 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     4501 tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat
     4561 aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac
     4621 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
     4681 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat
     4741 gcggcatcag agcagattgt actgagagtg caccatatcg acgctctccc ttatgcgact
     4801 cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga
     4861 atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac
     4921 ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga
     4981 tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc
     5041 gtccggcgta gaggatctgg ctagcgatga ccctgctgat tggttcgctg accatttccg
     5101 ggtgcgggac ggcgttacca gaaactcaga aggttcgtcc aaccaaaccg actctgacgg
     5161 cagtttacga gagagatgat agggtctgct tcagtaagcc agatgctaca caattaggct
     5221 tgtacatatt gtcgttagaa cgcggctaca attaatacat aaccttatgt atcatacaca
     5281 ta
//
