LOCUS       sequence-8255-ex        9641 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9641)
  AUTHORS   Luan H, Peabody NC, Vinson CR, White BH
  TITLE     Refined spatial manipulation of neuronal function by combinatorial 
            restriction of transgene expression.
  JOURNAL   Neuron. 2006 Nov 9. 52(3):425-36.
  PUBMED    17088209
REFERENCE   2  (bases 1 to 9641)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron. 
            2006 Nov 9. 52(3):425-36."
COMMENT     Sequence Label: pCaST-dVP16AD
FEATURES             Location/Qualifiers
     source          1..9641
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(235..823)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(315..334)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(994..1785,1786..1854))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1617..1636
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1855..1959)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2027..2045
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2083..2105)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2205..2224
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    join(2761..2791,2792..2993)
                     /label=P element 3' end
                     /note="P element 3' end"
     primer_bind     3015..3035
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        3017..3035
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     3064..3080
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     3065..3081
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             3726..3746
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          4270..4335
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4465..4485
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    4757..4891
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4794..4813)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4848..4867
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4918..4936)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(5396..5467,5862..6135,6210..6864,6926..7241,7462..7593,
                     7664..8278)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLLPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     misc_feature    join(9056..9610,9611..9641)
                     /label=P element 5' end
ORIGIN
        1 ggccagaccc acgtagtcca gcggcagatc ggcggcggag aagttaagcg tctccaggat
       61 gaccttgccc gaactggggc acgtggtgtt cgacgatgtg cagctaattt cgcccggctc
      121 cacgtccgcc cattggttaa tcagcagacc ctcgttggcg taacggaacc atgagaggta
      181 cgacaaccat ttgaggtata ctggcaccga gcccgagttc aagaagaagg cgtttttcca
      241 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
      301 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
      361 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
      421 gctttctcaa tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
      481 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
      541 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
      601 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
      661 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
      721 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
      781 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
      841 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
      901 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
      961 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     1021 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     1081 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     1141 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     1201 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     1261 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     1321 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     1381 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     1441 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     1501 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     1561 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa
     1621 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     1681 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     1741 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     1801 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     1861 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     1921 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     1981 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
     2041 gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
     2101 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
     2161 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat
     2221 tgtactgaga gtgcaccata tgcggtgtga aataccgcac cgaatcgcgc ggaactaacg
     2281 acagtcgctc caaggtcgtc gaacaaaagg tgaatgtgtt gcggagagcg ggtgggagac
     2341 agcgaaagag caactacgaa acgtggtgtg gtggaggtga attatgaaga gggcgcgcga
     2401 tttgaaaagt atgtatataa aaaatatatc ccggtgtttt atgtagcgat aaacgagttt
     2461 ttgatgtaag gtatgcaggt gtgtaagtct tttggttaga agacaaatcc aaagtctact
     2521 tgtggggatg ttcgaagggg aaatacttgt attctatagg tcatatcttg tttttattgg
     2581 cacaaatata attacattag ctttttgagg gggcaataaa cagtaaacac gatggtaata
     2641 atggtaaaaa aaaaaacaag cagttatttc ggatatatgt cggctactcc ttgcgtcggg
     2701 cccgaagtct tagagccaga tatgcgagca cccggaagct cacgatgaga atggccagac
     2761 catgatgaaa taacataagg tggtcccgtc ggcaagagac atccacttaa cgtatgcttg
     2821 caataagtgc gagtgaaagg aatagtattc tgagtgtcgt attgagtctg agtgagacag
     2881 cgatatgatt gttgattaac ccttagcatg tccgtggggt ttgaattaac tcataatatt
     2941 aattagacga aattattttt aaagttttat ttttaataat ttgcgagtac gcaaagcttg
     3001 gctgcatcca acgcgcaatt aaccctcact aaagggaaca aaagctgggt accgggcccc
     3061 ccctcgaggt cgacggtatc gataagcttg atatcgaatt ccctaggaga tctgtttcta
     3121 gagagagtcg cctcgaaatt gcctctgttt gttcggaaaa gtctagtgga agtgtgagga
     3181 aaagtttcgg aaaatgcaca ctcaagcgcg taggagcgat ctgtgagtgt gttctttcgg
     3241 atatatccga tcggatcggg tctttataaa tagatataaa cgcctcagtt tgctcgcgag
     3301 cgaattgtgg ccaatgcagc tgcactgaac gaaattagtg gcaattgtgt gggattttcg
     3361 tccactttaa gtctgcccaa gttgcgtttc aaattgaatt tgcagtggtt tttattacac
     3421 tcggcaaggg caaggaccat ccttcacacg tcaatgcaat gttaatgtgc gggggcgacc
     3481 cggctgggaa aggtcgggaa aaacacaaaa agccattaag tggcaaccaa aatacattcc
     3541 aggcccagct ggtggtcctg tcgccttctg aaagcaacaa gtctcacatt gcgtagccga
     3601 gatgttgacc aaggcccaag ctgcggccac tttcatttgg ctttcgagtg ttaagcgcgt
     3661 cggttgctaa gtggatcgaa acgcggccgc aaaacatgga taaagcggaa ttaattcccg
     3721 agcctccaaa aaagaagaga aaggtcgaat tgggtaccgc cccccccacc gacgtgtccc
     3781 tgggcgatga gctgcacctg gatggcgagg atgtggccat ggcccacgcc gatgccctgg
     3841 atgacttcga cctggatatg ctgggcgatg gcgacagccc cggccccggc ttcacccccc
     3901 acgatagcgc cccctacggc gccctggaca tggccgattt cgagttcgag caaatgttca
     3961 ccgacgccct gggcatcgat gagtacggcg gcgctagcgg aggaggtggt ggaggtggag
     4021 gtggaggtac tagtctggag atcgaggccg ccttcctgga gcgcgagaac accgccctgg
     4081 agacccgcgt ggccgagctg cgccagcgcg tgcagcgcct gcgcaaccgc gtgagccagt
     4141 accgcacccg ctacggcccc ctgggcggcg gcaagtaagg cgcgcctcta gaggatcttt
     4201 gtgaaggaac cttacttctg tggtgtgaca taattggaca aactacctac agagatttaa
     4261 agctctaagg taaatataaa atttttaagt gtataatgtg ttaaactact gattctaatt
     4321 gtttgtgtat tttagattcc aacctatgga actgatgaat gggagcagtg gtggaatgcc
     4381 tttaatgagg aaaacctgtt ttgctcagaa gaaatgccat ctagtgatga tgaggctact
     4441 gctgactctc aacattctac tcctccaaaa aagaagagaa aggtagaaga ccccaaggac
     4501 tttccttcag aattgctaag ttttttgagt catgctgtgt ttagtaatag aactcttgct
     4561 tgctttgcta tttacaccac aaaggaaaaa gctgcactgc tatacaagaa aattatggaa
     4621 aaatatttga tgtatagtgc cttgactaga gatcataatc agccatacca catttgtaga
     4681 ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa
     4741 tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     4801 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     4861 actcatcaat gtatcttatc atgtctggat cggatccact agaaggcctt agtatgtatg
     4921 taagttaata aaaccctttt ttggagaatg tagatttaaa aaaacatatt ttttttttat
     4981 tttttactgc actggacatc attgaactta tctgatcagt tttaaattta cttcgatcca
     5041 agggtatttg aagtaccagg ttctttcgat tacctctcac tcaaaatgac attccactca
     5101 aagtcagcgc tgtttgcctc cttctctgtc cacagaaata tcgccgtctc tttcgccgct
     5161 gcgtccgcta tctctttcgc caccgtttgt agcgttacct agcgtcaatg tccgccttca
     5221 gttgcacttt gtcagcggtt tcgtgacgaa gctccaagcg gtttacgcca tcaattaaac
     5281 acaaagtgct gtgccaaaac tcctctcgct tcttattttt gtttgttttt tgagtgattg
     5341 gggtggtgat tggttttggg tgggtaagca ggggaaagtg tgaaaaatcc cggcaatggg
     5401 ccaagaggat caggagctat taattcgcgg aggcagcaaa cacccatctg ccgagcatct
     5461 gaacaatgtg agtagtacat gtgcatacat cttaagttca cttgatctat aggaactgcg
     5521 attgcaacat caaattgtct gcggcgtgag aactgcgacc cacaaaaatc ccaaaccgca
     5581 atcgcacaaa caaatagtga cacgaaacag attattctgg tagctgtgct cgctatataa
     5641 gacaattttt aagatcatat catgatcaag acatctaaag gcattcattt tcgactacat
     5701 tcttttttac aaaaaatata acaaccagat attttaagct gatcctagat gcacaaaaaa
     5761 taaataaaag tataaaccta cttcgtagga tacttcgttt tgttcggggt tagatgagca
     5821 taacgcttgt agttgatatt tgagatcccc tatcattgca gggtgacagc ggagcggctt
     5881 cgcagagctg cattaaccag ggcttcgggc aggccaaaaa ctacggcacg ctcctgccac
     5941 ccagtccgcc ggaggactcc ggttcaggga gcggccaact agccgagaac ctcacctatg
     6001 cctggcacaa tatggacatc tttggggcgg tcaatcagcc gggctccgga tggcggcagc
     6061 tggtcaaccg gacacgcgga ctattctgca acgagcgaca cataccggcg cccaggaaac
     6121 atttgctcaa gaacggtgag tttctattcg cagtcggctg atctgtgtga aatcttaata
     6181 aagggtccaa ttaccaattt gaaactcagt ttgcggcgtg gcctatccgg gcgaactttt
     6241 ggccgtgatg ggcagttccg gtgccggaaa gacgaccctg ctgaatgccc ttgcctttcg
     6301 atcgccgcag ggcatccaag tatcgccatc cgggatgcga ctgctcaatg gccaacctgt
     6361 ggacgccaag gagatgcagg ccaggtgcgc ctatgtccag caggatgacc tctttatcgg
     6421 ctccctaacg gccagggaac acctgatttt ccaggccatg gtgcggatgc cacgacatct
     6481 gacctatcgg cagcgagtgg cccgcgtgga tcaggtgatc caggagcttt cgctcagcaa
     6541 atgtcagcac acgatcatcg gtgtgcccgg cagggtgaaa ggtctgtccg gcggagaaag
     6601 gaagcgtctg gcattcgcct ccgaggcact aaccgatccg ccgcttctga tctgcgatga
     6661 gcccacctcc ggactggact catttaccgc ccacagcgtc gtccaggtgc tgaagaagct
     6721 gtcgcagaag ggcaagaccg tcatcctgac cattcatcag ccgtcttccg agctgtttga
     6781 gctctttgac aagatccttc tgatggccga gggcagggta gctttcttgg gcactcccag
     6841 cgaagccgtc gacttctttt cctagtgagt tcgatgtgtt tattaagggt atctagcatt
     6901 acattacatc tcaactccta tccagcgtgg gtgcccagtg tcctaccaac tacaatccgg
     6961 cggactttta cgtacaggtg ttggccgttg tgcccggacg ggagatcgag tcccgtgatc
     7021 ggatcgccaa gatatgcgac aattttgcta ttagcaaagt agcccgggat atggagcagt
     7081 tgttggccac caaaaatttg gagaagccac tggagcagcc ggagaatggg tacacctaca
     7141 aggccacctg gttcatgcag ttccgggcgg tcctgtggcg atcctggctg tcggtgctca
     7201 aggaaccact cctcgtaaaa gtgcgactta ttcagacaac ggtgagtggt tccagtggaa
     7261 acaaatgata taacgcttac aattcttgga aacaaattcg ctagatttta gttagaattg
     7321 cctgattcca cacccttctt agtttttttc aatgagatgt atagtttata gttttgcaga
     7381 aaataaataa atttcattta actcgcgaac atgttgaaga tatgaatatt aatgagatgc
     7441 gagtaacatt ttaatttgca gatggttgcc atcttgattg gcctcatctt tttgggccaa
     7501 caactcacgc aagtgggcgt gatgaatatc aacggagcca tcttcctctt cctgaccaac
     7561 atgacctttc aaaacgtctt tgccacgata aatgtaagtc ttgtttagaa tacatttgca
     7621 tattaataat ttactaactt tctaatgaat cgattcgatt taggtgttca cctcagagct
     7681 gccagttttt atgagggagg cccgaagtcg actttatcgc tgtgacacat actttctggg
     7741 caaaacgatt gccgaattac cgctttttct cacagtgcca ctggtcttca cggcgattgc
     7801 ctatccgatg atcggactgc gggccggagt gctgcacttc ttcaactgcc tggcgctggt
     7861 cactctggtg gccaatgtgt caacgtcctt cggatatcta atatcctgcg ccagctcctc
     7921 gacctcgatg gcgctgtctg tgggtccgcc ggttatcata ccattcctgc tctttggcgg
     7981 cttcttcttg aactcgggct cggtgccagt atacctcaaa tggttgtcgt acctctcatg
     8041 gttccgttac gccaacgagg gtctgctgat taaccaatgg gcggacgtgg agccgggcga
     8101 aattagctgc acatcgtcga acaccacgtg ccccagttcg ggcaaggtca tcctggagac
     8161 gcttaacttc tccgccgccg atctgccgct ggactacgtg ggtctggcca ttctcatcgt
     8221 gagcttccgg gtgctcgcat atctggctct aagacttcgg gcccgacgca aggagtagcc
     8281 gacatatatc cgaaataact gcttgttttt ttttttacca ttattaccat cgtgtttact
     8341 gtttattgcc ccctcaaaaa gctaatgtaa ttatatttgt gccaataaaa acaagatatg
     8401 acctatagaa tacaagtatt tccccttcga acatccccac aagtagactt tggatttgtc
     8461 ttctaaccaa aagacttaca cacctgcata ccttacatca aaaactcgtt tatcgctaca
     8521 taaaacaccg ggatatattt tttatataca tacttttcaa atcgcgcgcc ctcttcataa
     8581 ttcacctcca ccacaccacg tttcgtagtt gctctttcgc tgtctcccac ccgctctccg
     8641 caacacattc accttttgtt cgacgacctt ggagcgactg tcgttagttc cgcgcgattc
     8701 ggttcgctca aatggttccg agtggttcat ttcgtctcaa tagaaattag taataaatat
     8761 ttgtatgtac aatttatttg ctccaatata tttgtatata tttccctcac agctatattt
     8821 attctaattt aatattatga ctttttaagg taattttttg tgacctgttc ggagtgatta
     8881 gcgttacaat ttgaactgaa agtgacatcc agtgtttgtt ccttgtgtag atgcatctca
     8941 aaaaaatggt gggcataata gtgttgttta tatatatcaa aaataacaac tataataata
     9001 agaatacatt taatttagaa aatgcttgga tttcactgga actagaatta attcggctgc
     9061 tgctctaaac gacgcatttc gtactccaaa gtacgaattt tttccctcaa gctcttattt
     9121 tcattaaaca atgaacagga cctaacgcac agtcacgtta ttgtttacat aaatgatttt
     9181 ttttactatt caaacttact ctgtttgtgt actcccactg gtatagcctt cttttatctt
     9241 ttctggttca ggctctatca ctttactagg tacggcatct gcgttgagtc gcctcctttt
     9301 aaatgtctga ccttttgcag gtgcagcctt ccactgcgaa tcattaaagt gggtatcaca
     9361 aatttgggag ttttcaccaa ggctgcaccc aaggctctgc tcccacaatt ttctcttaat
     9421 agcacacttc ggcacgtgaa ttaattttac tccagtcaca gctttgcagc aaaatttgca
     9481 atatttcatt tttttttatt ccacgtaagg gttaatgttt tcaaaaaaaa attcgtccgc
     9541 acacaacctt tcctctcaac aagcaaacgt gcactgaatt taagtgtata cttcggtaag
     9601 cttcggctat cgacgggacc accttatgtt atttcatcat g
//
