LOCUS       sequence-82560-e        6648 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A secreted Annexin V (sAnxV::GFP) Phosphatidylserine  sensor .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6648)
  AUTHORS   Mapes J, Chen YZ, Kim A, Mitani S, Kang BH, Xue D
  TITLE     CED-1, CED-7, and TTR-52 regulate surface phosphatidylserine 
            expression on apoptotic and phagocytic cells.
  JOURNAL   Curr Biol. 2012 Jul 24;22(14):1267-75. doi: 
            10.1016/j.cub.2012.05.052. Epub 2012 Jun 21.
  PUBMED    22727702
REFERENCE   2  (bases 1 to 6648)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2012.05.052"; journalName: "Curr Biol"; date: 
            "2012-07-24- 24"; volume: "22"; issue: "14"; pages: "1267-75"
COMMENT     Sequence Label: pJM31
FEATURES             Location/Qualifiers
     source          1..6648
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1649..1677)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     2425..2445
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     complement(4222..4244)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4344..4363
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4441..4459)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4527..4631
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4632..4700,4701..5492)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4850..4869)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5663..6251
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6152..6171
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6405..6422
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6539..6560
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6575..6580,6581..6598,6599..6605)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6613..6629
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6618..6640
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     join(6637..6648,1..5)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     join(6637..6648,1..5)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 atgaccatga ttacgccaag cttgcatgcc tgcaggtcga ctctagagga tcaccaaaaa
       61 cggaacgttg agctggacgg aaatagtggt aaagtgacat gattatagtt tgaagatttc
      121 taatttcaca attagagcaa atgttgttcg gtatttattt tcaacggtat ttatactatt
      181 ttccaccttt ttctagaaca ttcgagctgc ttgttgcaaa aggagggcga ctcacattcg
      241 gtacatggaa aagtagtgta cacaataaag agacccagat acattttccg tctgcgtctc
      301 tttgcaccca ccgggagtat tttcaaacga atgcatctag gaccttctag aacattctgt
      361 aaggctgcag aatgcgggta tataaggaaa gcgggctcag aggaagccaa cacgctttgt
      421 tctagtgcat ctaaaaaact tcgaaaatcc tcatcgggat ccccgggatt ggccaaagga
      481 cccaaaggta tgtttcgaat gatactaaca taacatagaa cattttcagg aggacccttg
      541 gctagcaaaa atgcataagg ttttgctggc actgttcttt atctttctgg caccagcatc
      601 cgcactggca gtctccgaac cggtcctagc gtcgacggta ccggcacagg ttctcagagg
      661 cactgtgact gacttccctg gatttgatga gcgggctgat gcagaaactc ttcggaaggc
      721 tatgaaaggc ttgggcacag atgaggagag catcctgact ctgttgacat cccgaagtaa
      781 tgctcagcgc caggaaatct ctgcagcttt taagactctg tttggcaggg atcttctgga
      841 tgacctgaaa tcagaactaa ctggaaaatt tgaaaaatta attgtggctc tgatgaaacc
      901 ctctcggctt tatgatgctt atgaactgaa acatgccttg aagggagctg gaacaaatga
      961 aaaagtactg acagaaatta ttgcttcaag gacacctgaa gaactgagag ccatcaaaca
     1021 agtttatgaa gaagaatatg gctcaagcct ggaagatgac gtggtggggg acacttcagg
     1081 gtactaccag cggatgttgg tggttctcct tcaggctaac agagaccctg atgctggaat
     1141 tgatgaagct caagttgaac aagatgctca ggctttattt caggctggag aacttaaatg
     1201 ggggacagat gaagaaaagt ttatcaccat ctttggaaca cgaagtgtgt ctcatttgag
     1261 aaaggtgttt gacaagtaca tgactatatc aggatttcaa attgaggaaa ccattgaccg
     1321 cgagacttct ggcaatttag agcaactact ccttgctgtt gtgaaatcta ttcgaagtat
     1381 acctgcctac cttgcagaga ccctctatta tgctatgaag ggagctggga cagatgatca
     1441 taccctcatc agagtcatgg tttccaggag tgagattgat ctgtttaaca tcaggaagga
     1501 gtttaggaag aattttgcca cctctcttta ttccatgatt aagggagata catctgggga
     1561 ctataagaaa gctcttctgc tgctctgtgg agaagatgac acggtaccgg tagaaaaaat
     1621 gagtaaagga gaagaacttt tcactggagt tgtcccaatt cttgttgaat tagatggtga
     1681 tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcaa catacggaaa
     1741 acttaccctt aaatttattt gcactactgg aaaactacct gttccatggg taagtttaaa
     1801 catatatata ctaactaacc ctgattattt aaattttcag ccaacacttg tcactacttt
     1861 cacttatggt gttcaatgct tctcgagata cccagatcat atgaaacggc atgacttttt
     1921 caagagtgcc atgcccgaag gttatgtaca ggaaagaact atatttttca aagatgacgg
     1981 gaactacaag acacgtaagt ttaaacagtt cggtactaac taaccataca tatttaaatt
     2041 ttcaggtgct gaagtcaagt ttgaaggtga tacccttgtt aatagaatcg agttaaaagg
     2101 tattgatttt aaagaagatg gaaacattct tggacacaaa ttggaataca actataactc
     2161 acacaatgta tacatcatgg cagacaaaca aaagaatgga atcaaagttg taagtttaaa
     2221 catgatttta ctaactaact aatctgattt aaattttcag aacttcaaaa ttagacacaa
     2281 cattgaagat ggaagcgttc aactagcaga ccattatcaa caaaatactc caattggcga
     2341 tggccctgtc cttttaccag acaaccatta cctgtccaca caatctgccc tttcgaaaga
     2401 tcccaacgaa aagagagacc acatggtcct tcttgagttt gtaacagctg ctgggattac
     2461 acatggcatg gatgaactat acaaatagca ttcgtagaat tccaactgag cgccggtcgc
     2521 taccattacc aacttgtctg gtgtcaaaaa taataggggc cgctgtcatc agagtaagtt
     2581 taaactgagt tctactaact aacgagtaat atttaaattt tcagcatctc gcgcccgtgc
     2641 ctctgacttc taagtccaat tactcttcaa catccctaca tgctctttct ccctgtgctc
     2701 ccacccccta tttttgttat tatcaaaaaa acttcttctt aatttctttg ttttttagct
     2761 tcttttaagt cacctctaac aatgaaattg tgtagattca aaaatagaat taattcgtaa
     2821 taaaaagtcg aaaaaaattg tgctccctcc ccccattaat aataattcta tcccaaaatc
     2881 tacacaatgt tctgtgtaca cttcttatgt tttttttact tctgataaat tttttttgaa
     2941 acatcataga aaaaaccgca cacaaaatac cttatcatat gttacgtttc agtttatgac
     3001 cgcaattttt atttcttcgc acgtctgggc ctctcatgac gtcaaatcat gctcatcgtg
     3061 aaaaagtttt ggagtatttt tggaattttt caatcaagtg aaagtttatg aaattaattt
     3121 tcctgctttt gctttttggg ggtttcccct attgtttgtc aagagtttcg aggacggcgt
     3181 ttttcttgct aaaatcacaa gtattgatga gcacgatgca agaaagatcg gaagaaggtt
     3241 tgggtttgag gctcagtgga aggtgagtag aagttgataa tttgaaagtg gagtagtgtc
     3301 tatggggttt ttgccttaaa tgacagaata cattcccaat ataccaaaca taactgtttc
     3361 ctactagcgt cgacggtacc atggtattga tatctgagct ccgcatcggc cgctgtcatc
     3421 agatcgccat ctcgcgcccg tgcctctgac ttctaagtcc aattactctt caacatccct
     3481 acatgctctt tctccctgtg ctcccacccc ctatttttgt tattatcaaa aaaacttctt
     3541 cttaatttct ttgtttttta gcttctttta agtcacctct aacaatgaaa ttgtgtagat
     3601 tcaaaaatag aattaattcg taataaaaag tcgaaaaaaa ttgtgctccc tccccccatt
     3661 aataataatt ctatcccaaa atctacacaa tgttctgtgt acacttctta tgtttttttt
     3721 acttctgata aatttttttt gaaacatcat agaaaaaacc gcacacaaaa taccttatca
     3781 tatgttacgt ttcagtttat gaccgcaatt tttatttctt cgcacgtctg ggcctctcat
     3841 gacgtcaaat catgctcatc gtgaaaaagt tttggagtat ttttggaatt tttcaatcaa
     3901 gtgaaagttt atgaaattaa ttttcctgct tttgcttttt gggggtttcc cctattgttt
     3961 gtcaagagtt tcgaggacgg cgtttttctt gctaaaatca caagtattga tgagcacgat
     4021 gcaagaaaga tcggaagaag gtttgggttt gaggctcagt ggaaggtgag tagaagttga
     4081 taatttgaaa gtggagtagt gtctatgggg tttttgcctt aaatgacaga atacattccc
     4141 aatataccaa acataactgt ttcctactag tcggccgtac gggccctttc gtctcgcgcg
     4201 tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg
     4261 tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg
     4321 gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat
     4381 gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc ggccttaagg
     4441 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
     4501 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     4561 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     4621 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     4681 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     4741 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     4801 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     4861 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     4921 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     4981 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     5041 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     5101 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     5161 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     5221 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     5281 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     5341 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     5401 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     5461 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     5521 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     5581 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     5641 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     5701 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     5761 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     5821 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     5881 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     5941 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     6001 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagcattgag
     6061 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     6121 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     6181 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     6241 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     6301 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     6361 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     6421 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     6481 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     6541 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     6601 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagct
//
