LOCUS       sequence-82563-e        5724 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A secreted Lactadherin (sGFP::LactC1C2) Phosphatidylserine sensor .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5724)
  AUTHORS   Mapes J, Chen YZ, Kim A, Mitani S, Kang BH, Xue D
  TITLE     CED-1, CED-7, and TTR-52 regulate surface phosphatidylserine 
            expression on apoptotic and phagocytic cells.
  JOURNAL   Curr Biol. 2012 Jul 24;22(14):1267-75. doi: 
            10.1016/j.cub.2012.05.052. Epub 2012 Jun 21.
  PUBMED    22727702
REFERENCE   2  (bases 1 to 5724)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2012.05.052"; journalName: "Curr Biol"; date: 
            "2012-07-24- 24"; volume: "22"; issue: "14"; pages: "1267-75"
COMMENT     Sequence Label: pJM483
FEATURES             Location/Qualifiers
     source          1..5724
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(665..693)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     1441..1461
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     complement(3298..3320)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3420..3439
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(3517..3535)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3603..3707
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3708..3776,3777..4568)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3926..3945)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4739..5327
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5228..5247
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5481..5498
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5615..5636
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5651..5656,5657..5674,5675..5681)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5689..5705
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5694..5716
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 aagcttgcat gcctgcaggt cgactctaga ggatcaccaa aaacggaacg ttgagctgga
       61 cggaaatagt ggtaaagtga catgattata gtttgaagat ttctaatttc acaattagag
      121 caaatgttgt tcggtattta ttttcaacgg tatttatact attttccacc tttttctaga
      181 acattcgagc tgcttgttgc aaaaggaggg cgactcacat tcggtacatg gaaaagtagt
      241 gtacacaata aagagaccca gatacatttt ccgtctgcgt ctctttgcac ccaccgggag
      301 tattttcaaa cgaatgcatc taggaccttc tagaacattc tgtaaggctg cagaatgcgg
      361 gtatataagg aaagcgggct cagaggaagc caacacgctt tgttctagtg catctaaaaa
      421 acttcgaaaa tcctcatcgg atccccggga ttggccaaag gacccaaagg tatgtttcga
      481 atgatactaa cataacatag aacattttca ggaggaccct tggctagcaa aaatgcataa
      541 ggttttgctg gcactgttct ttatctttct ggcaccagca tccgcactgg cagtctccga
      601 accggtccta gcgtcgacgg taccggtaga aaaaatgagt aaaggagaag aacttttcac
      661 tggagttgtc ccaattcttg ttgaattaga tggtgatgtt aatgggcaca aattttctgt
      721 cagtggagag ggtgaaggtg atgcaacata cggaaaactt acccttaaat ttatttgcac
      781 tactggaaaa ctacctgttc catgggtaag tttaaacata tatatactaa ctaaccctga
      841 ttatttaaat tttcagccaa cacttgtcac tactttctgt tatggtgttc aatgcttctc
      901 gagataccca gatcatatga aacggcatga ctttttcaag agtgccatgc ccgaaggtta
      961 tgtacaggaa agaactatat ttttcaaaga tgacgggaac tacaagacac gtaagtttaa
     1021 acagttcggt actaactaac catacatatt taaattttca ggtgctgaag tcaagtttga
     1081 aggtgatacc cttgttaata gaatcgagtt aaaaggtatt gattttaaag aagatggaaa
     1141 cattcttgga cacaaattgg aatacaacta taactcacac aatgtataca tcatggcaga
     1201 caaacaaaag aatggaatca aagttgtaag tttaaacatg attttactaa ctaactaatc
     1261 tgatttaaat tttcagaact tcaaaattag acacaacatt gaagatggaa gcgttcaact
     1321 agcagaccat tatcaacaaa atactccaat tggcgatggc cctgtccttt taccagacaa
     1381 ccattacctg tccacacaat ctgccctttc gaaagatccc aacgaaaaga gagaccacat
     1441 ggtccttctt gagtttgtaa cagctgctgg gattacacat ggcatggatg aactatacaa
     1501 acctggcggc cgctgttcta cacagctggg catggaaggg ggcgccattg ctgattcaca
     1561 gatttccgcc tcgtctgtgt atatgggttt catgggcttg cagcgctggg gcccggagct
     1621 ggctcgtctg taccgcacag ggatcgtcaa tgcctggaca gccagcaact atgatagcaa
     1681 gccctggatc caggtgaacc ttctgcggaa gatgcgggta tcaggtgtga tgacgcaggg
     1741 tgccagccgt gccgggaggg cggagtacct gaagaccttc aaggtggctt acagcctcga
     1801 cggacgcaag tttgagttca tccaggatga aagcggtgga gacaaggagt ttttgggtaa
     1861 cctggacaac aacagcctga aggttaacat gttcaacccg actctggagg cacagtacat
     1921 aaggctgtac cctgtttcgt gccaccgcgg ctgcaccctc cgcttcgagc tcctgggctg
     1981 tgagttgcac ggatgttctg agcccctggg cctgaagaat aacacaattc ctgacagcca
     2041 gatgtcagcc tccagcagct acaagacatg gaacctgcgt gcttttggct ggtaccccca
     2101 cttgggaagg ctggataatc agggcaagat caatgcctgg acggctcaga gcaacagtgc
     2161 caaggaatgg ctgcaggttg acctgggcac tcagaggcaa gtgacaggaa tcatcaccca
     2221 gggggcccgt gactttggcc acatccagta tgtggcgtcc tacaaggtag cccacagtga
     2281 tgatggtgtg cagtggactg tatatgagga gcaaggaagc agcaaggtct tccagggcaa
     2341 cttggacaac aactcccaca agaagaacat cttcgagaaa cccttcatgg ctcgctacgt
     2401 gcgtgtcctt ccagtgtcct ggcataaccg catcaccctg cgcctggagc tgctgggctg
     2461 ttaagatatc tgagctccgc atcggccgct gtcatcagat cgccatctcg cgcccgtgcc
     2521 tctgacttct aagtccaatt actcttcaac atccctacat gctctttctc cctgtgctcc
     2581 caccccctat ttttgttatt atcaaaaaaa cttcttctta atttctttgt tttttagctt
     2641 cttttaagtc acctctaaca atgaaattgt gtagattcaa aaatagaatt aattcgtaat
     2701 aaaaagtcga aaaaaattgt gctccctccc cccattaata ataattctat cccaaaatct
     2761 acacaatgtt ctgtgtacac ttcttatgtt ttttttactt ctgataaatt ttttttgaaa
     2821 catcatagaa aaaaccgcac acaaaatacc ttatcatatg ttacgtttca gtttatgacc
     2881 gcaattttta tttcttcgca cgtctgggcc tctcatgacg tcaaatcatg ctcatcgtga
     2941 aaaagttttg gagtattttt ggaatttttc aatcaagtga aagtttatga aattaatttt
     3001 cctgcttttg ctttttgggg gtttccccta ttgtttgtca agagtttcga ggacggcgtt
     3061 tttcttgcta aaatcacaag tattgatgag cacgatgcaa gaaagatcgg aagaaggttt
     3121 gggtttgagg ctcagtggaa ggtgagtaga agttgataat ttgaaagtgg agtagtgtct
     3181 atggggtttt tgccttaaat gacagaatac attcccaata taccaaacat aactgtttcc
     3241 tactagtcgg ccgtacgggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct
     3301 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag
     3361 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc
     3421 ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg
     3481 cgtaaggaga aaataccgca tcaggcggcc ttaagggcct cgtgatacgc ctatttttat
     3541 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
     3601 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     3661 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     3721 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     3781 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     3841 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     3901 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     3961 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     4021 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     4081 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     4141 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     4201 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     4261 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     4321 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     4381 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     4441 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     4501 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     4561 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     4621 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     4681 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     4741 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     4801 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     4861 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca
     4921 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     4981 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     5041 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     5101 acaccgaact gagataccta cagcgtgagc attgagaaag cgccacgctt cccgaaggga
     5161 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     5221 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     5281 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     5341 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     5401 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     5461 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     5521 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
     5581 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
     5641 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
     5701 aacaatttca cacaggaaac agct
//
