LOCUS       sequence-82568-e       12814 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Produces the carotenoid violaxanthin in E. coli..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12814)
  AUTHORS   Neuman H, Galpaz N, Cunningham FX Jr, Zamir D, Hirschberg J
  TITLE     The tomato mutation nxd1 reveals a gene necessary for neoxanthin 
            biosynthesis and demonstrates that violaxanthin is a sufficient 
            precursor for abscisic acid biosynthesis.
  JOURNAL   Plant J. 2014 Apr;78(1):80-93. doi: 10.1111/tpj.12451. Epub 2014 Mar
            12.
  PUBMED    24506237
REFERENCE   2  (bases 1 to 12814)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.12451";
            journalName: "Plant J"; date: "2014-04"; volume: "78"; issue: "1"; 
            pages: "80-93"
COMMENT     Sequence Label: pAC-VIOL
FEATURES             Location/Qualifiers
     source          1..12814
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     134..153
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(220..322)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(714..731)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(848..1393)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(928..947)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     rep_origin      complement(1493..1948)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1630..1651)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1842..1861
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     terminator      complement(1985..2032)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     2028..2046
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(2099..2116)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             3958..3980
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    3995..4019
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(4019..4038)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4020..4038)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4126..4145)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        join(4279..4284,4285..4301,4302..4307)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     CDS             complement(join(12374..12814,1..219))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 gaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt
       61 gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt
      121 ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga
      181 tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga
      241 aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt
      301 ggaacctctt acgtgccgat caacgtctca ttttcgccaa aagttggccc agggcttccc
      361 ggtatcaaca gggacaccag gatttattta ttctgcgaag tgatcttccg tcacaggtat
      421 ttattcggcg caaagtgcgt cgggtgatgc tgccaactta ctgatttagt gtatgatggt
      481 gtttttgagg tgctccagtg gcttctgttt ctatcagctg tccctcctgt tcagctactg
      541 acggggtggt gcgtaacggc aaaagcaccg ccggacatca gcgctagcgg agtgtatact
      601 ggcttactat gttggcactg atgagggtgt cagtgaagtg cttcatgtgg caggagaaaa
      661 aaggctgcac cggtgcgtca gcagaatatg tgatacagga tatattccgc ttcctcgctc
      721 actgactcgc tacgctcggt cgttcgactg cggcgagcgg aaatggctta cgaacggggc
      781 ggagatttcc tggaagatgc caggaagata cttaacaggg aagtgagagg gccgcggcaa
      841 agccgttttt ccataggctc cgcccccctg acaagcatca cgaaatctga cgctcaaatc
      901 agtggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggcggctccc
      961 tcgtgcgctc tcctgttcct gcctttcggt ttaccggtgt cattccgctg ttatggccgc
     1021 gtttgtctca ttccacgcct gacactcagt tccgggtagg cagttcgctc caagctggac
     1081 tgtatgcacg aaccccccgt tcagtccgac cgctgcgcct tatccggtaa ctatcgtctt
     1141 gagtccaacc cggaaagaca tgcaaaagca ccactggcag cagccactgg taattgattt
     1201 agaggagtta gtcttgaagt catgcgccgg ttaaggctaa actgaaagga caagttttgg
     1261 tgactgcgct cctccaagcc agttacctcg gttcaaagag ttggtagctc agagaacctt
     1321 cgaaaaaccg ccctgcaagg cggttttttc gttttcagag caagagatta cgcgcagacc
     1381 aaaacgatct caagaagatc atcttattac atgagcggat acatatttga atgtatttag
     1441 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgaaattgta
     1501 aacgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac
     1561 caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg
     1621 agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa
     1681 gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt
     1741 tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt
     1801 agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga
     1861 gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc
     1921 gcgcttaatg cgccgctaca gggcgcgtcc cattcgccaa tccggatata gttcctcctt
     1981 tcagcaaaaa acccctcaag acccgtttag aggccccaag gggttatgct agttattgct
     2041 cagcggtggc agcagccaac tcagcttcct ttcgggcttt gttagcagcc ggatctcagt
     2101 ggtggtggtg gtggtgctcg acttcaagct gtctgaagta atttatcgtt gttactctca
     2161 ttcttcctcg tcgatttcgg agttttcctg attactttta ccctaaacgc cgccttctta
     2221 tctgaaccaa actcgatgat gtcggacgat ctaaaccgcg cgggaaaatt cggtgttgct
     2281 ctatatcttc ttccttcgtt atcggtcaca taggttccgt gttcgcttcg aagatccatc
     2341 aagaagaaag ctccgtcttt gtaaatcaca cgagcatgca tcttcgaaac ctgagacgaa
     2401 gggatcacaa tgcgcattcc aggaaaatct tgatctggtt cgcttccgac gatgcaaggt
     2461 tgatcttcat ctttggttag acataatgtt tccgaaacgc aacaatcgtc gccgtgtgga
     2521 attagatacc attctccctt tatagtacgt tcaagagcat cgtcatcttc aaaccactct
     2581 cgaagccggt catcggcttt gtcagtgagt ctgcaactag gtggccttcc ttggagtttt
     2641 tcactgttac ctccaaggac ccagtcaagc atcgatggca tagcaatgtc aacgaagaat
     2701 ctaccaccaa ctcttcctgg atgtggtact ctaaactttg tcaagaaaga cagaggacca
     2761 agcccaacac ctaagtatgc tttgtaagtg gaagccataa ttgcagccat cctcgccatt
     2821 gcatggataa tagcgactct cagtcttcta gattcctcat atcttttcaa agaggaaaca
     2881 acatcaacag gtgtagtcgt ttcaacactc tgtttccatg cttcatcaag ctccaatgct
     2941 agttgaaaac tatcctcaat ggccatgcat ccaccttgac ccatatttgg ctgcatcgca
     3001 tggatagaat ccccgagcag cgtaacacgc cctttacccc aagtaaaacc aggacttcta
     3061 tcataaatat ctcttctcag aatggcttcc tcctcagtcg catgcaacaa gtcgagtaca
     3121 ttgtcgcacc aaccgtcaaa tatttcaaac aaccttttct tcataccatt tggagcatca
     3181 gccccaccag ctggttcctc gtgaaatgca taccattgca tttttccacc accaacatcc
     3241 gaagaaacaa agtactgttt gtgtcccaag aaaacccggt agccaacaga ctcgatatcc
     3301 gctggtataa aatctgcaat ccccgtgtaa caagtgtagc ctgaataagt agcttcacta
     3361 cggccaaaca aattatttct caccttagac caaatgccat ctgcacccac aagcagatca
     3421 ccttcatagc gttgaccatt ctcgagtacc acagtaacct tatctccaga atcttcaaaa
     3481 tcaacaacat tactctcgtt tctaatcaca tcttctccaa ccgcacgtgc tagaatctgc
     3541 tgcagagtca ttctactaat tactctagtc acaggaagtc cccgtgacgc cgcaggagtg
     3601 aaagtatcaa actttacata ccaagtacca gagataccat caacgagacc gttaatccga
     3661 tcaccagtga tacacccagc ttccataact tgttcagcaa cttcaatatc aatagcttcc
     3721 aaagcagcta aagcgttgct ctgtatttga atcgggcctc tgtattttcc ttctcctctt
     3781 atagcactca aatctttctc aaacactaac acatcgaatc ctttcttctt agccgctaaa
     3841 gcaaacacca atcctccgat tccacctccg gcaactaaaa ccctcgattt cttcttctcc
     3901 gtcaccgcct ctctcttctc ctccttctca actaacgccg tcgccgccat atgtatatct
     3961 ccttcttaaa gttaaacaaa attatttcta gaggggaatt gttatccgct cacaattccc
     4021 ctatagtgag tcgtattaat ttcgcgggat cgagatcgat ctcgatcctc tacgccggac
     4081 gcatcgtggc cggcatcacc ggcgccacag gtgcggttgc tggcgcctat atcgccgaca
     4141 tcaccgatgg ggaagatcgg gctcgccact tcgggctcat gtaatcagat aaaatatttc
     4201 tagatttcag tgcaatttat ctcttcaaat gtagcacctg aagtcagccc catacgatat
     4261 aagttgtaat tctcatgttt gacagcttat catcgataag ctttaatgcg gtagtttatc
     4321 acagttaaat tgctaacgca gtcaggcacc gtgtatgaaa tctaacaatg cgctcatcgt
     4381 catcctcggc accgtcaccc tggatgctgt aggcataggc ttggttatgc cggtactgcc
     4441 gggcctcttg cgggatatcg tccattccga cagcatcgcc agtcactatg gcgtgctgct
     4501 agcgctatat gcgttgatgc aatttctatg cgcacccgtt ctcggagcac tgtccgaccg
     4561 ctttggccgc cgcccagtcc tgctcgcttc gctacttgga gccactatcg actacgcgat
     4621 catggcgacc acacccgtcc tgtggatcta aaggcacagc gtctcatgct tcgcacaatg
     4681 taaaactgct tcagaacctg gcgagagcta tccgcgcggt ctacggttaa ctgatactaa
     4741 aagacaattc agcgggtaac cttgcaatgg tgagtggcag taaagcgggc gtttcgcctc
     4801 atcgcgaaat agaagtaatg agacaatcca ttgacgatca cctggctggc ctgttacctg
     4861 aaaccgacag ccaggatatc gtcagccttg cgatgcgtga aggcgtcatg gcacccggta
     4921 aacggatccg tccgctgctg atgctgctgg ccgcccgcga cctccgctac cagggcagta
     4981 tgcctacgct gctcgatctc gcctgcgccg ttgaactgac ccataccgcg tcgctgatgc
     5041 tcgacgacat gccctgcatg gacaacgccg agctgcgccg cggtcagccc actacccaca
     5101 aaaaatttgg tgagagcgtg gcgatccttg cctccgttgg gctgctctct aaagcctttg
     5161 gtctgatcgc cgccaccggc gatctgccgg gggagaggcg tgcccaggcg gtcaacgagc
     5221 tctctaccgc cgtgggcgtg cagggcctgg tactggggca gtttcgcgat cttaacgatg
     5281 ccgccctcga ccgtacccct gacgctatcc tcagcaccaa ccacctcaag accggcattc
     5341 tgttcagcgc gatgctgcag atcgtcgcca ttgcttccgc ctcgtcgccg agcacgcgag
     5401 agacgctgca cgccttcgcc ctcgacttcg gccaggcgtt tcaactgctg gacgatctgc
     5461 gtgacgatca cccggaaacc ggtaaagatc gcaataagga cgcgggaaaa tcgacgctgg
     5521 tcaaccggct gggcgcagac gcggcccggc aaaagctgcg cgagcatatt gattccgccg
     5581 acaaacacct cacttttgcc tgtccgcagg gcggcgccat ccgacagttt atgcatctgt
     5641 ggtttggcca tcaccttgcc gactggtcac cggtcatgaa aatcgcctga taccgccctt
     5701 ttgggttcaa gcagtacata acgatggaac cacattacag gagtagtgat gaatgaagga
     5761 cgagcgcctt gttcagcgta agaacgatca tctggatatc gttctcgacc cccgtcgcgc
     5821 cgtaactcag gctagcgcag gttttgagcg ctggcgcttt acccactgcg ccctgccaga
     5881 gctgaatttt agcgacatca cgctggaaac caccttcctg aatcggcagc tacaggctcc
     5941 gctgctgatc agctccatga ccggcggcgt tgagcgctcg cgccatatca accgccacct
     6001 cgccgaggcg gcgcaggtgc taaaaattgc gatgggggtg ggctcccagc gcgtcgccat
     6061 tgagagcgac gcgggcttag ggctggataa aaccctgcgg cagctggctc cggacgtgcc
     6121 gctgctggcg aacctcggcg cggcgcagct gaccggcaga aaaggtattg attacgcccg
     6181 acgggccgtg gagatgatcg aggcggatgc gctgattgtg cacctaaacc cgctgcagga
     6241 ggcgctacag cccggcggcg atcgcgactg gcgcggacgg ctggcggcta ttgaaactct
     6301 ggtccgcgag ctgcccgttc cgctggtggt gaaagaggtg ggagccggta tctcccgaac
     6361 cgtggccggg cagctgatcg atgccggcgt taccgtgatt gacgtcgcgg gcgcgggcgg
     6421 caccagctgg gccgccgttg aaggcgagcg ggcggccacc gagcagcagc gcagcgtggc
     6481 caacgtcttt gccgactggg ggatccccac cgctgaggcg ctggttgaca ttgccgaggc
     6541 ctggccgcag atgcccctta ttgcctcggg cgggattaaa aacggcgtcg acgcggcgaa
     6601 agcgctgcgg ctcggcgcgt gcatggtagg gcaggccgcc gccgtgctcg gcagcgcagg
     6661 cgtctccacg gagaaggtga tcgatcactt caacgtgatt attgagcagc tgcgggtggc
     6721 ctgcttctgc accggcagcc gcagcctgag cgatctaaag caggctgata tccgctatgt
     6781 gcgggatacg ccatgagcca ttttgccatt gtggcaccgc cgctctacag tcatgcggtg
     6841 gcgctgcatg ccctgtggga gcactgcgct atcagccgcc gccgcaggta gaacgctcgc
     6901 cacgcagcac gccgcggatc tttgcctcgc tgggcaccct ccagggccac cgtctacgcc
     6961 tgtttcagaa gatcgcccgc gcctgtgcca gcgtgggggc ggaggtgacc attgcccact
     7021 gcgatggcct gacgcccgcc caggccgact cgctctacgc ctgcggcgcg acggaggtgg
     7081 tcagctttgt cgaccagccg cgctacgttg ccgaggctaa tctggtgatc acccacggcg
     7141 gtctcaatac cgtactggat gcgctggctg ccgcgacgcc ggtgctggcg gtgccactct
     7201 ctttcgacca gcccgccgtg gctgcccggc tggtctataa cgggctgggt cgccgggtat
     7261 cgcgctttgc cagacagcag acgctggcgg atgagattgc ccaactgctg ggggatgaga
     7321 cgctgcatca gcgtctggcg acggcccgcc agcagcttaa cgacgccggg ggcacgcccc
     7381 gtgcggcgac cctgattgaa caggccatag cagggagtga gagcgtatcg tgagggatct
     7441 gattttagtc ggcggcggcc tggccaacgg gctgatcgcc tggcgtctgc gccagcgcta
     7501 cccgcagctt aacctgctgc tgatcgaggc cggggagcag cccggcggga accatacctg
     7561 gtcattccat gaagacgatc tgactcccgg gcagcacgcc tggctggccc cgctggtggc
     7621 ccacgcctgg ccgggctatg aggtgcagtt tcccgatctt cgccgtcgcc tcgcgcgcgg
     7681 ctactactcc attacctcag agcgctttgc cgaggccctg catcaggcgc tgggggagaa
     7741 catctggcta aactgttcgg tgagcgaggt gttacccaat agcgtgcgcc ttgccaacgg
     7801 tgaggcgctg cttgccggag cggtgattga cggacgcggc gtgaccgcca gttcggcgat
     7861 gcaaaccggc tatcagctct ttcttggtca gcagtggcgg ctgacacagc cccacggcct
     7921 gaccgtaccg atcctgatgg atgccacggt ggcgcagcag cagggctatc gctttgtcta
     7981 cacgctgccg ctctccgccg acacgctgct gatcgaggat acgcgctacg ccaatgtccc
     8041 gcagcgtgat gataatgccc tacgccagac ggttaccgac tatgctcaca gcaaagggtg
     8101 gcagctggcc cagcttgaac gcgaggagac cggctgtctg ccgattaccc tggcgggtga
     8161 catccaggct ctgtgggccg atgcgccggg cgtgccgcgc tcgggaatgc gggctgggct
     8221 atttcaccct accactggct attcgctgcc gctggcggtg gcccttgccg acgcgattgc
     8281 cgacagcccg cggctgggca gcgttccgct ctatcagctc acccggcagt ttgccgaacg
     8341 ccactggcgc aggcagggat tcttccgcct gctgaaccgg atgcttttcc tggccgggcg
     8401 cgaggagaac cgctggcggg tgatgcagcg cttttatggg ctgccggagc ccaccgtaga
     8461 gcgcttttac gccggtcggc tctctctctt tgataaggcc cgcattttga cgggcaagcc
     8521 accggttccg ctgggcgaag cctggcgggc ggcgctgaac cattttcctg acagacgaga
     8581 taaaggatga aaaaaaccgt tgtgattggc gcaggctttg gtggcctggc gctggcgatt
     8641 cgcctgcagg cggcagggat cccaaccgta ctgctggagc agcgggacaa gcccggcggt
     8701 cgggcctacg tctggcatga ccagggcttt acctttgacg ccgggccgac ggtgatcacc
     8761 gatcctaccg cgcttgaggc gctgttcacc ctggccggca ggcgcatgga ggattacgtc
     8821 aggctgctgc cggtaaaacc cttctaccga ctctgctggg agtccgggaa gaccctcgac
     8881 tatgctaacg acagcgccga gcttgaggcg cagattaccc agttcaaccc ccgcgacgtc
     8941 gagggctacc ggcgctttct ggcttactcc caggcggtat tccaggaggg atatttgcgc
     9001 ctcggcagcg tgccgttcct ctcttttcgc gacatgctgc gcgccgggcc gcagctgctt
     9061 aagctccagg cgtggcagag cgtctaccag tcggtttcgc gctttattga ggatgagcat
     9121 ctgcggcagg ccttctcgtt ccactccctg ctggtaggcg gcaacccctt caccacctcg
     9181 tccatctaca ccctgatcca cgcccttgag cgggagtggg gggtctggtt ccctgagggc
     9241 ggcaccgggg cgctggtgaa cggcatggtg aagctgttta ccgatctggg cggggagatc
     9301 gaactcaacg cccgggtcga agagctggtg gtggccgata accgcgtaag ccaggtccgg
     9361 ctggcggatg gtcggatctt tgacaccgac gccgtagcct cgaacgctga cgtggtgaac
     9421 acctataaaa agctgctcgg ccaccatccg gtggggcaga agcgggcggc agcgctggag
     9481 cgcaagagca tgagcaactc gctgtttgtg ctctacttcg gcctgaacca gcctcattcc
     9541 cagctggcgc accataccat ctgttttggt ccccgctacc gggagctgat cgacgagatc
     9601 tttaccggca gcgcgctggc ggatgacttc tcgctctacc tgcactcgcc ctgcgtgacc
     9661 gatccctcgc tcgcgcctcc cggctgcgcc agcttctacg tgctggcccc ggtgccgcat
     9721 cttggcaacg cgccgctgga ctgggcgcag gaggggccga agctgcgcga ccgcatcttt
     9781 gactaccttg aagagcgcta tatgcccggc ctgcgtagcc agctggtgac ccagcggatc
     9841 tttaccccgg cagacttcca cgacacgctg gatgcgcatc tgggatcggc cttctccatc
     9901 gagccgctgc tgacccaaag cgcctggttc cgcccgcaca accgcgacag cgacattgcc
     9961 aacctctacc tggtgggcgc aggtactcac cctggggcgg gcattcctgg cgtagtggcc
    10021 tcggcgaaag ccaccgccag cctgatgatt gaggatctgc aatgagccaa ccgccgctgc
    10081 ttgaccacgc cacgcagacc atggccaacg gctcgaaaag ttttgccacc gctgcgaagc
    10141 tgttcgaccc ggccacccgc cgtagcgtgc tgatgctcta cacctggtgc cgccactgcg
    10201 atgacgtcat tgacgaccag acccacggct tcgccagcga ggccgcggcg gaggaggagg
    10261 ccacccagcg cctggcccgg ctgcgcacgc tgaccctggc ggcgtttgaa ggggccgaga
    10321 tgcaggatcc ggccttcgct gcctttcagg aggtggcgct gacccacggt attacgcccc
    10381 gcatggcgct cgatcacctc gacggctttg cgatggacgt ggctcagacc cgctatgtca
    10441 cctttgagga tacgctgcgc tactgctatc acgtggcggg cgtggtgggt ctgatgatgg
    10501 ccagggtgat gggcgtgcgg gatgagcggg tgctggatcg cgcctgcgat ctggggctgg
    10561 ccttccagct gacgaatatc gcccgggata ttattgacga tgcggctatt gaccgctgct
    10621 atctgcccgc cgagtggctg caggatgccg ggctgacccc ggagaactat gccgcgcggg
    10681 agaatcgggc cgcgctggcg cgggtggcgg agcggcttat tgatgccgca gagccgtact
    10741 acatctcctc ccaggccggg ctacacgatc tgccgccgcg ctgcgcctgg gcgatcgcca
    10801 ccgcccgcag cgtctaccgg gagatcggta ttaaggtaaa agcggcggga ggcagcgcct
    10861 gggatcgccg ccagcacacc agcaaaggtg aaaaaattgc catgctgatg gcggcaccgg
    10921 ggcaggttat tcgggcgaag acgacgaggg tgacgccgcg tccggccggt ctttggcagc
    10981 gtcccgttta ggcgggcggc catgacgttc acgcaggatc gcctgtaggt cggcaggctt
    11041 gcgggcgtaa ataaaaccga aggagacgca gccctcccgg ccgcgcaccg cgtggtgcag
    11101 gcggtgggcg acgtagagcc gcttcaggta gccccggcgc gggatccagt ggaagggcca
    11161 gcgctgatgc accagaccgt cgtgcaccag gaagtagagc aggccataga ccgtcatgcc
    11221 gcagccaatc cactgcaggg gccaaacgcc cgccgtgccc acggcaatca gcgcgatagc
    11281 caccccggca aacaccaccg caaagagatc gtttagctca aatacgccct tgcgcggggt
    11341 atggtgtgac tcatgccagc gccatcccca gccgtgcata atgtagcggt gggtaaacgc
    11401 ggcgatgccc tccatcgcaa taacgctcaa gatgacgatt aaactattta ctagcatagc
    11461 ggtgcgcgct cctgcgactc agactggctt aatcaaaacc cttagcctct aagcatagtc
    11521 gcaatcgcag gcggcgcgca tcggtatcag gccagagcct gctgcagatc cgccagcaga
    11581 tccttctcat cctcaatgcc caccgacagg cggatcagct gcggcgtaat gccgttcgcc
    11641 aggcgctgcg ccagcgggat cgaggcgtgg gtcatactga acggctggct caccaggctc
    11701 tcaacgccgc ccaggctctc agcgagggta aacagcttca gtttactgat cacctctgcc
    11761 gcacgctggt cgtcgccttt gatcacgatg gagatcatgc cgcccggctg agccatctgc
    11821 tggcgcgcga gggcatactg cgggtgggat gtcagccacg ggaaccagac tttctcgacc
    11881 tgcggctgct gctcaagcca gcgggccagc gccagggcgt tgctgctgtg gcgctcgatg
    11941 cgcagggcga gggtgcggat cccgcgcagg gtcaggaagc tgctgaacgg atcgagcacg
    12001 ccgcccaccg cattttgcag gtagcccagc ttctccgcca gcgccggacg atcgcccacc
    12061 accgccagcc cggcgaccac gtcggagtgg ccgttgaggt atttggtggc cgagtgcacc
    12121 acgatatcaa agcccagcgt cagcgggcgg tggatcagcg gcgaggcaaa ggtattatct
    12181 gccacgctaa tcacgttatg acggcgggcg atctcagcga tagccgccag gtccgccagc
    12241 ttcaacagcg ggttggtggg ggtctctacc cgggtcgaat ttgctttcga atttctgcca
    12301 ttcatccgct tattatcact tattcaggcg tagcaccagg cgtttaaggg caccaataac
    12361 tgccttaaaa aaattacgcc ccgccctgcc actcatcgca gtactgttgt aattcattaa
    12421 gcattctgcc gacatggaag ccatcacaga cggcatgatg aacctgaatc gccagcggca
    12481 tcagcacctt gtcgccttgc gtataatatt tgcccatggt gaaaacgggg gcgaagaagt
    12541 tgtccatatt ggccacgttt aaatcaaaac tggtgaaact cacccaggga ttggctgaga
    12601 cgaaaaacat attctcaata aaccctttag ggaaataggc caggttttca ccgtaacacg
    12661 ccacatcttg cgaatatatg tgtagaaact gccggaaatc gtcgtggtat tcactccaga
    12721 gcgatgaaaa cgtttcagtt tgctcatgga aaacggtgta acaagggtga acactatccc
    12781 atatcaccag ctcaccgtct ttcattgcca tacg
//
