LOCUS       sequence-8257-ex       13975 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13975)
  AUTHORS   Luan H, Peabody NC, Vinson CR, White BH
  TITLE     Refined spatial manipulation of neuronal function by combinatorial 
            restriction of transgene expression.
  JOURNAL   Neuron. 2006 Nov 9. 52(3):425-36.
  PUBMED    17088209
REFERENCE   2  (bases 1 to 13975)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron. 
            2006 Nov 9. 52(3):425-36."
COMMENT     Sequence Label: pCaST-elav-VP16AD
FEATURES             Location/Qualifiers
     source          1..13975
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(235..823)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(315..334)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(994..1785,1786..1854))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1617..1636
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1855..1959)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2027..2045
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2083..2105)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2205..2224
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    join(2761..2791,2792..2993)
                     /label=P element 3' end
                     /note="P element 3' end"
     primer_bind     3015..3035
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        3017..3035
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     3064..3080
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     3065..3081
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             6749..6769
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6782..7015
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     regulatory      7829..7838
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7835..8512
                     /codon_start=1
                     /product="improved tetrameric variant of DsRed fluorescent 
                     protein"
                     /label=DsRed2
                     /note="mammalian codon-optimized"
                     /translation="MASSENVITEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     ATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGETHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTEGRHH
                     LFL"
     primer_bind     complement(8015..8035)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     8427..8450
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     intron          8604..8669
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             8799..8819
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    9091..9225
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9128..9147)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9182..9201
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9252..9270)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(9730..9801,10196..10469,10544..11198,11260..11575,
                     11796..11927,11998..12612)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLLPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     misc_feature    join(13390..13944,13945..13975)
                     /label=P element 5' end
ORIGIN
        1 ggccagaccc acgtagtcca gcggcagatc ggcggcggag aagttaagcg tctccaggat
       61 gaccttgccc gaactggggc acgtggtgtt cgacgatgtg cagctaattt cgcccggctc
      121 cacgtccgcc cattggttaa tcagcagacc ctcgttggcg taacggaacc atgagaggta
      181 cgacaaccat ttgaggtata ctggcaccga gcccgagttc aagaagaagg cgtttttcca
      241 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
      301 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
      361 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
      421 gctttctcaa tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
      481 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
      541 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
      601 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
      661 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
      721 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
      781 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
      841 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
      901 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
      961 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     1021 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     1081 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     1141 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     1201 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     1261 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     1321 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     1381 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     1441 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     1501 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     1561 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa
     1621 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     1681 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     1741 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     1801 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     1861 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     1921 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     1981 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
     2041 gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
     2101 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
     2161 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat
     2221 tgtactgaga gtgcaccata tgcggtgtga aataccgcac cgaatcgcgc ggaactaacg
     2281 acagtcgctc caaggtcgtc gaacaaaagg tgaatgtgtt gcggagagcg ggtgggagac
     2341 agcgaaagag caactacgaa acgtggtgtg gtggaggtga attatgaaga gggcgcgcga
     2401 tttgaaaagt atgtatataa aaaatatatc ccggtgtttt atgtagcgat aaacgagttt
     2461 ttgatgtaag gtatgcaggt gtgtaagtct tttggttaga agacaaatcc aaagtctact
     2521 tgtggggatg ttcgaagggg aaatacttgt attctatagg tcatatcttg tttttattgg
     2581 cacaaatata attacattag ctttttgagg gggcaataaa cagtaaacac gatggtaata
     2641 atggtaaaaa aaaaaacaag cagttatttc ggatatatgt cggctactcc ttgcgtcggg
     2701 cccgaagtct tagagccaga tatgcgagca cccggaagct cacgatgaga atggccagac
     2761 catgatgaaa taacataagg tggtcccgtc ggcaagagac atccacttaa cgtatgcttg
     2821 caataagtgc gagtgaaagg aatagtattc tgagtgtcgt attgagtctg agtgagacag
     2881 cgatatgatt gttgattaac ccttagcatg tccgtggggt ttgaattaac tcataatatt
     2941 aattagacga aattattttt aaagttttat ttttaataat ttgcgagtac gcaaagcttg
     3001 gctgcatcca acgcgcaatt aaccctcact aaagggaaca aaagctgggt accgggcccc
     3061 ccctcgaggt cgacggtatc gataagcttg atatcgaatt ccccggatcc agtcgaggga
     3121 tcactgaact aaaaagtaaa gagaggagaa acaggagaag cccttaaagt gcgagttcgc
     3181 tggagtgcct atcgatcctt gagggcagcg ctcgatcctt attactattg gcaggatcat
     3241 tttatacccc ttacataaaa atatcagtat cagtttgttc ccttatcatg cgtgctcttt
     3301 gacatatccg ggaatttatt tctatttttg cttagtcaaa ctgaagacca ttttgtccca
     3361 acataaaata tgtaaaatat ttttttttat cattttaaat tctcagggat taagtcaatg
     3421 tttgaaaact tctagctgac ttgcgctcca ctaccactcg gcatgcgtgc ccctgattag
     3481 taggccaggt ggtgggcctt tggcacgatc gcaaccctaa ggactgcaac cgaatagcat
     3541 tgagaaggag accaggattt ctgcactcaa aggtgaaatt gtctgagcac ggaacacgga
     3601 aaaacaacaa caaaaacatg gcagacggaa aacaatgccg ccagcggcaa ggagggaggg
     3661 tggaaacaca tgccacgatt cagtgtcgaa tgtcctgtga tcccacattc atatattttg
     3721 ttattgtgtg cctgcatccc tcgcctgctt ttaattcctg catcgatcct tctttgctcc
     3781 tggtctacga acccgatgtc ttatggtaat tttatgatta ttacaattag attcggggga
     3841 aactgcatct gccgccgtct gcagatccga ttcatttgga ttccgtctgc tcaatccaga
     3901 tccatgatgg gatcatggcc atttgggcat tggtacatgg aaagttaaaa gcgccctagg
     3961 cgttctaatc caactcgtct caagaaaaac gggtgaatgc atggaacgct ggatctggtt
     4021 ctggaagatc cggctaagtc aacaagaaat atgtaacctc gccattatag ctttactttc
     4081 gaagcagaaa atgcaatcga gaatatcgaa tgcacgctcc aggttcctat ggctcctagc
     4141 tttcggtgca gcacaacaaa tatttgggtt ggttcgtttt tccgccacga ttttaaaacg
     4201 ctaagggtgg ctttttcgga gtccctctca tttgagccgg gaaaaagacc agaccctccc
     4261 tttccctccc cagccacttt ccagctttgt tgtaacctcg tgttgtgcct gctttttaat
     4321 cccgctttgt tgtaacgcag gccatttgct gtttttgttt tgtttctggc gtggctaaaa
     4381 cggattatgt gacccaaccc tttcgatttc gagtctcatt gtattacatc cttttcccaa
     4441 aaatgactaa aaaagcgtat tgcctatttc tggggtggac agaatgaaac ggggtcagca
     4501 cgtggccgca aacttgaaat accattatgg atccaatagg actgtgaaga gttattagtg
     4561 aaacatttcg aacaaactat tcctttaatt gaaatttgcc aattccccac ttaagcatcg
     4621 catttgcata tttgtatgct aaccaaactc tctgcgacgt cgatctgtct gaatctgaat
     4681 ccgcatatct ttcgagggca gcaatagcgc caccccaccc catttccgtt gccactagca
     4741 gcgcaccacc ctttgaaagg acatcaaaaa ccaccccctg cccaaagttg cgttacaatt
     4801 cccgaagtgc agtgcagcgt acataaaaca tacaatgaaa tccgataaaa ttttctatgt
     4861 cctttatatc ctcataacat gcccaactat aaaccgagcc aaattcttct cgtgaaaaag
     4921 ttgcacacaa cgcgatttca ctgtacctta cattgttctg taaactggaa cagagcgtcc
     4981 gactggaaga ggcgaaggaa aaaatcagca cagggaacag gaagttcaca aggattgggg
     5041 gtgtccgagg gtggctacat atatatggcg atatatggca tacagtatgt gcatacatat
     5101 acatacatat acacatatat gtatgtatgt accatactat gtggcgtgtc tagtggcgcg
     5161 aataacgaat acaggagcaa tgaagcacac gaacgaagcg aaacgaaacg aagggaaata
     5221 ttgaaaaagc cagaattgca ttacttcaaa actataccgg ctcttggata tgggtcctgg
     5281 gtctcggttc cttgcttctg gctttcgctt cgtagggggt gtacgagcaa tactgccagg
     5341 agaaaccacc ctagcctttg gcgttggtgg ttgatagtgc aaaaataata ataacggaaa
     5401 cggaaccata aacccaaaac agcccttaca acgcgactga tttgtaagga taatggtcgc
     5461 atgcatcata aaacggttga cgacctcgtc ggaaagagcc aacattttgc aatgatttcc
     5521 aattgctcct gctatctagg cccaatgggc cgctaagtac atatgaagca tacatagcta
     5581 aatacatatg aagcatacat acatcgggtt aactataaaa ccaatgtgtt ctagatgagt
     5641 ttgttaattt tacaaagtaa gttagggctg ctccagtggt tttaagttgc ttaactggac
     5701 aagagaactt ggctctctcc cccatctatc tcttgtcttc gggaaaggta acctccattt
     5761 tgatcggttg aatttgtatc aaaaagcatg aaaaactttg aatacagcat aaaccactga
     5821 gagagcagcc gaaagccagg ggtgtatcaa aaggggttct tagaaatatt ccactttgca
     5881 acgattatag actcgtttct tatcagacaa aactttctcc acttctacgt ggtatttaca
     5941 gaaacaaaac ttaaggtaaa atgttaggtg gctatatatt aaagctatta aaatatatta
     6001 aaggtttccg gaataacccc ctatctcggc gcgcagatac gcccatgtcg ccactctttc
     6061 gctctctacc ttccactctc gctccgaaca ccattgcaac cccgagagag ctgctcgaga
     6121 ggcaactatg agatatgaga atgagaggtt gctctatgtg aaaatggaaa ccgaaaccgt
     6181 caatgcctca atttcgcatt tcattatttc atttccacgc caaccatcgt gcgccgcggt
     6241 ctgaacgctc ctgccacaga aaaagaagat aaaagcaagg aaaattctaa tcgaatacca
     6301 aatatcgtgc ttgtgtgctc tttccgcaat tgattttttt taagtagtgc atgacaataa
     6361 ccgttgagtt gactccaacc gaagtaacca taactggaag ctgacaacag ccattggccg
     6421 gaatctcatc tcacaccgag cgaaatacgg cgacgccagc gtttatttat taaaaacgat
     6481 tacattacaa caaacaccaa agtcctggac aaccgaagag agtgtgggag aaaatcgaag
     6541 cagagagcaa ggaggagcgc cgtgagagcg cgcgcgcaag gatttgagat tgcaaagaga
     6601 acttagcaat tagttcgctt tgtttgtcca gccaaacaac aacaacaacc aaccagctcc
     6661 ccaaaaccaa ctacacacat actcgctccc taaactaaaa caatagcggc cgcaaaacat
     6721 ggataaagcg gaattaattc ccgagcctcc aaaaaagaag agaaaggtcg aattgggtac
     6781 cgcccccccg accgatgtca gcctggggga cgagctccac ttagacggcg aggacgtggc
     6841 gatggcgcat gccgacgcgc tagacgattt cgatctggac atgttggggg acggggattc
     6901 cccgggtccg ggatttaccc cccacgactc cgccccctac ggcgctctgg atatggccga
     6961 cttcgagttt gagcagatgt ttaccgatgc ccttggaatt gacgagtacg gtggggctag
     7021 cggaggaggt ggtggaggtg gaggtggagg tactagtctg gagatcgagg ccgccttcct
     7081 ggagcgcgag aacaccgccc tggagacccg cgtggccgag ctgcgccagc gcgtgcagcg
     7141 cctgcgcaac cgcgtgagcc agaaccgcac ccgctacggc cccctgggcg gcggcaagta
     7201 aggcgcgcct ctaggatcca aatgcaattg gctaaaaacc gaaaccaaaa cgcaacaaag
     7261 tatacgaaac acttgtgaaa ccgtacaaac aattgtggaa aaaaaattaa aagattatta
     7321 agattgaagt ctcaataaac attagtgctt aaataaactt aaagcgaccc gcgtggagag
     7381 tgcagtaaaa agaataactt ttgaaataaa tatttaccaa acagaaaaat attttataaa
     7441 tatttaaata agtgaaaaac aaattggtta ctctgaaaca aagaatattc aaattggcgc
     7501 taaaacaaag gagaaaaaat ttcaagaata ttatacagat aataagacat atttaactat
     7561 ataaaaccaa acttaatcaa caaagacaaa ggagtgaaaa aaataaaaaa aaattttaaa
     7621 agagttaaaa aaaaattgtt tatatccaaa ggaggcaaag gaacagcaca gaaagcgagg
     7681 aaacactcaa ataaaatccg ccaaaaatcg cagatccctg gaaaccaatt cgtgtgaaat
     7741 cggtcaagcc cccaacgact tttagcccgt cccagacgga gcaccgccaa gattcttacc
     7801 gccagcagcg ccatgatcaa gctcggtcgc caccatggcc tcctccgaga acgtcatcac
     7861 cgagttcatg cgcttcaagg tgcgcatgga gggcaccgtg aacggccacg agttcgagat
     7921 cgagggcgag ggcgagggcc gcccctacga gggccacaac accgtgaagc tgaaggtgac
     7981 caagggcggc cccctgccct tcgcctggga catcctgtcc ccccagttcc agtacggctc
     8041 caaggtgtac gtgaagcacc ccgccgacat ccccgactac aagaagctgt ccttccccga
     8101 gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggcgtggcga ccgtgaccca
     8161 ggactcctcc ctgcaggacg gctgcttcat ctacaaggtg aagttcatcg gcgtgaactt
     8221 cccctccgac ggccccgtga tgcagaagaa gaccatgggc tgggaggcct ccaccgagcg
     8281 cctgtacccc cgcgacggcg tgctgaaggg cgagacccac aaggccctga agctgaagga
     8341 cggcggccac tacctggtgg agttcaagtc catctacatg gccaagaagc ccgtgcagct
     8401 gcccggctac tactacgtgg acgccaagct ggacatcacc tcccacaacg aggactacac
     8461 catcgtggag cagtacgagc gcaccgaggg ccgccaccac ctgttcctgt agcggcctcg
     8521 actagaggat ctttgtgaag gaaccttact tctgtggtgt gacataattg gacaaactac
     8581 ctacagagat ttaaagctct aaggtaaata taaaattttt aagtgtataa tgtgttaaac
     8641 tactgattct aattgtttgt gtattttaga ttccaaccta tggaactgat gaatgggagc
     8701 agtggtggaa tgcctttaat gaggaaaacc tgttttgctc agaagaaatg ccatctagtg
     8761 atgatgaggc tactgctgac tctcaacatt ctactcctcc aaaaaagaag agaaaggtag
     8821 aagaccccaa ggactttcct tcagaattgc taagtttttt gagtcatgct gtgtttagta
     8881 atagaactct tgcttgcttt gctatttaca ccacaaagga aaaagctgca ctgctataca
     8941 agaaaattat ggaaaaatat ttgatgtata gtgccttgac tagagatcat aatcagccat
     9001 accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg
     9061 aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac
     9121 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     9181 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatcggatc cactagaagg
     9241 ccttagtatg tatgtaagtt aataaaaccc ttttttggag aatgtagatt taaaaaaaca
     9301 tatttttttt ttatttttta ctgcactgga catcattgaa cttatctgat cagttttaaa
     9361 tttacttcga tccaagggta tttgaagtac caggttcttt cgattacctc tcactcaaaa
     9421 tgacattcca ctcaaagtca gcgctgtttg cctccttctc tgtccacaga aatatcgccg
     9481 tctctttcgc cgctgcgtcc gctatctctt tcgccaccgt ttgtagcgtt acctagcgtc
     9541 aatgtccgcc ttcagttgca ctttgtcagc ggtttcgtga cgaagctcca agcggtttac
     9601 gccatcaatt aaacacaaag tgctgtgcca aaactcctct cgcttcttat ttttgtttgt
     9661 tttttgagtg attggggtgg tgattggttt tgggtgggta agcaggggaa agtgtgaaaa
     9721 atcccggcaa tgggccaaga ggatcaggag ctattaattc gcggaggcag caaacaccca
     9781 tctgccgagc atctgaacaa tgtgagtagt acatgtgcat acatcttaag ttcacttgat
     9841 ctataggaac tgcgattgca acatcaaatt gtctgcggcg tgagaactgc gacccacaaa
     9901 aatcccaaac cgcaatcgca caaacaaata gtgacacgaa acagattatt ctggtagctg
     9961 tgctcgctat ataagacaat ttttaagatc atatcatgat caagacatct aaaggcattc
    10021 attttcgact acattctttt ttacaaaaaa tataacaacc agatatttta agctgatcct
    10081 agatgcacaa aaaataaata aaagtataaa cctacttcgt aggatacttc gttttgttcg
    10141 gggttagatg agcataacgc ttgtagttga tatttgagat cccctatcat tgcagggtga
    10201 cagcggagcg gcttcgcaga gctgcattaa ccagggcttc gggcaggcca aaaactacgg
    10261 cacgctcctg ccacccagtc cgccggagga ctccggttca gggagcggcc aactagccga
    10321 gaacctcacc tatgcctggc acaatatgga catctttggg gcggtcaatc agccgggctc
    10381 cggatggcgg cagctggtca accggacacg cggactattc tgcaacgagc gacacatacc
    10441 ggcgcccagg aaacatttgc tcaagaacgg tgagtttcta ttcgcagtcg gctgatctgt
    10501 gtgaaatctt aataaagggt ccaattacca atttgaaact cagtttgcgg cgtggcctat
    10561 ccgggcgaac ttttggccgt gatgggcagt tccggtgccg gaaagacgac cctgctgaat
    10621 gcccttgcct ttcgatcgcc gcagggcatc caagtatcgc catccgggat gcgactgctc
    10681 aatggccaac ctgtggacgc caaggagatg caggccaggt gcgcctatgt ccagcaggat
    10741 gacctcttta tcggctccct aacggccagg gaacacctga ttttccaggc catggtgcgg
    10801 atgccacgac atctgaccta tcggcagcga gtggcccgcg tggatcaggt gatccaggag
    10861 ctttcgctca gcaaatgtca gcacacgatc atcggtgtgc ccggcagggt gaaaggtctg
    10921 tccggcggag aaaggaagcg tctggcattc gcctccgagg cactaaccga tccgccgctt
    10981 ctgatctgcg atgagcccac ctccggactg gactcattta ccgcccacag cgtcgtccag
    11041 gtgctgaaga agctgtcgca gaagggcaag accgtcatcc tgaccattca tcagccgtct
    11101 tccgagctgt ttgagctctt tgacaagatc cttctgatgg ccgagggcag ggtagctttc
    11161 ttgggcactc ccagcgaagc cgtcgacttc ttttcctagt gagttcgatg tgtttattaa
    11221 gggtatctag cattacatta catctcaact cctatccagc gtgggtgccc agtgtcctac
    11281 caactacaat ccggcggact tttacgtaca ggtgttggcc gttgtgcccg gacgggagat
    11341 cgagtcccgt gatcggatcg ccaagatatg cgacaatttt gctattagca aagtagcccg
    11401 ggatatggag cagttgttgg ccaccaaaaa tttggagaag ccactggagc agccggagaa
    11461 tgggtacacc tacaaggcca cctggttcat gcagttccgg gcggtcctgt ggcgatcctg
    11521 gctgtcggtg ctcaaggaac cactcctcgt aaaagtgcga cttattcaga caacggtgag
    11581 tggttccagt ggaaacaaat gatataacgc ttacaattct tggaaacaaa ttcgctagat
    11641 tttagttaga attgcctgat tccacaccct tcttagtttt tttcaatgag atgtatagtt
    11701 tatagttttg cagaaaataa ataaatttca tttaactcgc gaacatgttg aagatatgaa
    11761 tattaatgag atgcgagtaa cattttaatt tgcagatggt tgccatcttg attggcctca
    11821 tctttttggg ccaacaactc acgcaagtgg gcgtgatgaa tatcaacgga gccatcttcc
    11881 tcttcctgac caacatgacc tttcaaaacg tctttgccac gataaatgta agtcttgttt
    11941 agaatacatt tgcatattaa taatttacta actttctaat gaatcgattc gatttaggtg
    12001 ttcacctcag agctgccagt ttttatgagg gaggcccgaa gtcgacttta tcgctgtgac
    12061 acatactttc tgggcaaaac gattgccgaa ttaccgcttt ttctcacagt gccactggtc
    12121 ttcacggcga ttgcctatcc gatgatcgga ctgcgggccg gagtgctgca cttcttcaac
    12181 tgcctggcgc tggtcactct ggtggccaat gtgtcaacgt ccttcggata tctaatatcc
    12241 tgcgccagct cctcgacctc gatggcgctg tctgtgggtc cgccggttat cataccattc
    12301 ctgctctttg gcggcttctt cttgaactcg ggctcggtgc cagtatacct caaatggttg
    12361 tcgtacctct catggttccg ttacgccaac gagggtctgc tgattaacca atgggcggac
    12421 gtggagccgg gcgaaattag ctgcacatcg tcgaacacca cgtgccccag ttcgggcaag
    12481 gtcatcctgg agacgcttaa cttctccgcc gccgatctgc cgctggacta cgtgggtctg
    12541 gccattctca tcgtgagctt ccgggtgctc gcatatctgg ctctaagact tcgggcccga
    12601 cgcaaggagt agccgacata tatccgaaat aactgcttgt tttttttttt accattatta
    12661 ccatcgtgtt tactgtttat tgccccctca aaaagctaat gtaattatat ttgtgccaat
    12721 aaaaacaaga tatgacctat agaatacaag tatttcccct tcgaacatcc ccacaagtag
    12781 actttggatt tgtcttctaa ccaaaagact tacacacctg cataccttac atcaaaaact
    12841 cgtttatcgc tacataaaac accgggatat attttttata tacatacttt tcaaatcgcg
    12901 cgccctcttc ataattcacc tccaccacac cacgtttcgt agttgctctt tcgctgtctc
    12961 ccacccgctc tccgcaacac attcaccttt tgttcgacga ccttggagcg actgtcgtta
    13021 gttccgcgcg attcggttcg ctcaaatggt tccgagtggt tcatttcgtc tcaatagaaa
    13081 ttagtaataa atatttgtat gtacaattta tttgctccaa tatatttgta tatatttccc
    13141 tcacagctat atttattcta atttaatatt atgacttttt aaggtaattt tttgtgacct
    13201 gttcggagtg attagcgtta caatttgaac tgaaagtgac atccagtgtt tgttccttgt
    13261 gtagatgcat ctcaaaaaaa tggtgggcat aatagtgttg tttatatata tcaaaaataa
    13321 caactataat aataagaata catttaattt agaaaatgct tggatttcac tggaactaga
    13381 attaattcgg ctgctgctct aaacgacgca tttcgtactc caaagtacga attttttccc
    13441 tcaagctctt attttcatta aacaatgaac aggacctaac gcacagtcac gttattgttt
    13501 acataaatga ttttttttac tattcaaact tactctgttt gtgtactccc actggtatag
    13561 ccttctttta tcttttctgg ttcaggctct atcactttac taggtacggc atctgcgttg
    13621 agtcgcctcc ttttaaatgt ctgacctttt gcaggtgcag ccttccactg cgaatcatta
    13681 aagtgggtat cacaaatttg ggagttttca ccaaggctgc acccaaggct ctgctcccac
    13741 aattttctct taatagcaca cttcggcacg tgaattaatt ttactccagt cacagctttg
    13801 cagcaaaatt tgcaatattt catttttttt tattccacgt aagggttaat gttttcaaaa
    13861 aaaaattcgt ccgcacacaa cctttcctct caacaagcaa acgtgcactg aatttaagtg
    13921 tatacttcgg taagcttcgg ctatcgacgg gaccacctta tgttatttca tcatg
//
