LOCUS       sequence-82576-e         791 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses mOrange2 and PSD-95-EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Wickersham IR, Sullivan HA, Seung HS
  TITLE     Axonal and subcellular labelling using modified rabies viral 
            vectors.
  JOURNAL   Nat Commun. 2013;4:2332. doi: 10.1038/ncomms3332.
  PUBMED    23945836
REFERENCE   2  (bases 1 to 791)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2013"; volume: "4:2332. doi"; pages: " 
            10.1038/ncomms3332"
COMMENT     Sequence Label: pRVdG-1mOrange2-5PSD95EGFP
FEATURES             Location/Qualifiers
     source          1..791
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     51..70
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        51..69
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      217..226
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     636..655
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat taatacgact
       61 cactataggg agacccaagc tggctagatt aagcgtctga tgagtccgtg aggacgaaac
      121 ccggcgtacc gggtcacgct taacaaccag atcaaagaaa aaacagacat tgtcaattgc
      181 aaagcaaaaa tgtaacaccc ctacaccggg aaagccgcca ccatggtgag caagggcgag
      241 gagaataaca tggccatcat caaggagttc atgcgcttca aggtgcgcat ggagggctcc
      301 gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcttt
      361 cagaccgcta agctgaaggt gaccaagggt ggccccctgc ccttcgcctg ggacatcctg
      421 tcccctcatt tcacctacgg ctccaaggcc tacgtgaagc accccgccga catccccgac
      481 tacttcaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa ctacgaggac
      541 ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg acggcgagtt catctacaag
      601 gtgaagctgc gcggcaccaa cttcccctcc gacggccccg taatgcagaa gaagaccatg
      661 ggctgggagg cctcctccga gcggatgtac cccgaggacg gcgccctgaa gggcaagatc
      721 aagatgaggc tgaagctgaa ggacggcggc cactacacct ccgaggtcaa gaccacctac
      781 aaggccaaga a
//
