LOCUS       sequence-8258-ex        9227 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9227)
  AUTHORS   Luan H, Peabody NC, Vinson CR, White BH
  TITLE     Refined spatial manipulation of neuronal function by combinatorial 
            restriction of transgene expression.
  JOURNAL   Neuron. 2006 Nov 9. 52(3):425-36.
  PUBMED    17088209
REFERENCE   2  (bases 1 to 9227)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron. 
            2006 Nov 9. 52(3):425-36."
COMMENT     Sequence Label: X11-pCaST-Gal4DBD
FEATURES             Location/Qualifiers
     source          1..9227
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(235..823)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(315..334)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(994..1785,1786..1854))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1617..1636
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1855..1959)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2027..2045
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2083..2105)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2205..2224
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    join(2761..2791,2792..2993)
                     /label=P element 3' end
                     /note="P element 3' end"
     primer_bind     3015..3035
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        3017..3035
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     3064..3080
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     3065..3081
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             3321..3761
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     intron          3856..3921
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4051..4071
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    4343..4477
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4380..4399)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4434..4453
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4504..4522)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(4982..5053,5448..5721,5796..6450,6512..6827,7048..7179,
                     7250..7864)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLLPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     misc_feature    join(8642..9196,9197..9227)
                     /label=P element 5' end
ORIGIN
        1 ggccagaccc acgtagtcca gcggcagatc ggcggcggag aagttaagcg tctccaggat
       61 gaccttgccc gaactggggc acgtggtgtt cgacgatgtg cagctaattt cgcccggctc
      121 cacgtccgcc cattggttaa tcagcagacc ctcgttggcg taacggaacc atgagaggta
      181 cgacaaccat ttgaggtata ctggcaccga gcccgagttc aagaagaagg cgtttttcca
      241 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
      301 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
      361 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
      421 gctttctcaa tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
      481 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
      541 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
      601 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
      661 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
      721 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
      781 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
      841 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
      901 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
      961 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     1021 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     1081 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     1141 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     1201 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     1261 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     1321 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     1381 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     1441 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     1501 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     1561 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa
     1621 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     1681 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     1741 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     1801 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     1861 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     1921 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     1981 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
     2041 gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
     2101 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
     2161 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat
     2221 tgtactgaga gtgcaccata tgcggtgtga aataccgcac cgaatcgcgc ggaactaacg
     2281 acagtcgctc caaggtcgtc gaacaaaagg tgaatgtgtt gcggagagcg ggtgggagac
     2341 agcgaaagag caactacgaa acgtggtgtg gtggaggtga attatgaaga gggcgcgcga
     2401 tttgaaaagt atgtatataa aaaatatatc ccggtgtttt atgtagcgat aaacgagttt
     2461 ttgatgtaag gtatgcaggt gtgtaagtct tttggttaga agacaaatcc aaagtctact
     2521 tgtggggatg ttcgaagggg aaatacttgt attctatagg tcatatcttg tttttattgg
     2581 cacaaatata attacattag ctttttgagg gggcaataaa cagtaaacac gatggtaata
     2641 atggtaaaaa aaaaaacaag cagttatttc ggatatatgt cggctactcc ttgcgtcggg
     2701 cccgaagtct tagagccaga tatgcgagca cccggaagct cacgatgaga atggccagac
     2761 catgatgaaa taacataagg tggtcccgtc ggcaagagac atccacttaa cgtatgcttg
     2821 caataagtgc gagtgaaagg aatagtattc tgagtgtcgt attgagtctg agtgagacag
     2881 cgatatgatt gttgattaac ccttagcatg tccgtggggt ttgaattaac tcataatatt
     2941 aattagacga aattattttt aaagttttat ttttaataat ttgcgagtac gcaaagcttg
     3001 gctgcatcca acgcgcaatt aaccctcact aaagggaaca aaagctgggt accgggcccc
     3061 ccctcgaggt cgacggtatc gataagcttg atatcgaatt ccctaggaga tctgtttaaa
     3121 cgcggccgca aaacatgctg gagatccgcg ccgccttcct gcgccagcgc aacaccgccc
     3181 tgcgcaccga ggtggccgag ctggagcagg aggtgcagcg cctggagaac gaggtgagcc
     3241 agtacgagac ccgctacggc cccctgggcg gcggcaaggc tagcggagga ggtggtggag
     3301 gtggaggtgg aggtactagt atgaagctac tgtcttctat cgaacaagca tgcgatattt
     3361 gccgacttaa aaagctcaag tgctccaaag aaaaaccgaa gtgcgccaag tgtctgaaga
     3421 acaactggga gtgtcgctac tctcccaaaa ccaaaaggtc tccgctgact agggcacatc
     3481 tgacagaagt ggaatcaagg ctagaaagac tggaacagct atttctactg atttttcctc
     3541 gagaagacct tgacatgatt ttgaaaatgg attctttaca ggatataaaa gcattgttaa
     3601 caggattatt tgtacaagat aatgtgaata aagatgccgt cacagataga ttggcttcag
     3661 tggagactga tatgcctcta acattgagac agcatagaat aagtgcgaca tcatcatcgg
     3721 aagagagtag taacaaaggt caaagacagt tgactgtatc gtaaggcgcg cctctagagg
     3781 atctttgtga aggaacctta cttctgtggt gtgacataat tggacaaact acctacagag
     3841 atttaaagct ctaaggtaaa tataaaattt ttaagtgtat aatgtgttaa actactgatt
     3901 ctaattgttt gtgtatttta gattccaacc tatggaactg atgaatggga gcagtggtgg
     3961 aatgccttta atgaggaaaa cctgttttgc tcagaagaaa tgccatctag tgatgatgag
     4021 gctactgctg actctcaaca ttctactcct ccaaaaaaga agagaaaggt agaagacccc
     4081 aaggactttc cttcagaatt gctaagtttt ttgagtcatg ctgtgtttag taatagaact
     4141 cttgcttgct ttgctattta caccacaaag gaaaaagctg cactgctata caagaaaatt
     4201 atggaaaaat atttgatgta tagtgccttg actagagatc ataatcagcc ataccacatt
     4261 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa
     4321 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag
     4381 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     4441 gtccaaactc atcaatgtat cttatcatgt ctggatcgga tccactagaa ggccttagta
     4501 tgtatgtaag ttaataaaac ccttttttgg agaatgtaga tttaaaaaaa catatttttt
     4561 ttttattttt tactgcactg gacatcattg aacttatctg atcagtttta aatttacttc
     4621 gatccaaggg tatttgaagt accaggttct ttcgattacc tctcactcaa aatgacattc
     4681 cactcaaagt cagcgctgtt tgcctccttc tctgtccaca gaaatatcgc cgtctctttc
     4741 gccgctgcgt ccgctatctc tttcgccacc gtttgtagcg ttacctagcg tcaatgtccg
     4801 ccttcagttg cactttgtca gcggtttcgt gacgaagctc caagcggttt acgccatcaa
     4861 ttaaacacaa agtgctgtgc caaaactcct ctcgcttctt atttttgttt gttttttgag
     4921 tgattggggt ggtgattggt tttgggtggg taagcagggg aaagtgtgaa aaatcccggc
     4981 aatgggccaa gaggatcagg agctattaat tcgcggaggc agcaaacacc catctgccga
     5041 gcatctgaac aatgtgagta gtacatgtgc atacatctta agttcacttg atctatagga
     5101 actgcgattg caacatcaaa ttgtctgcgg cgtgagaact gcgacccaca aaaatcccaa
     5161 accgcaatcg cacaaacaaa tagtgacacg aaacagatta ttctggtagc tgtgctcgct
     5221 atataagaca atttttaaga tcatatcatg atcaagacat ctaaaggcat tcattttcga
     5281 ctacattctt ttttacaaaa aatataacaa ccagatattt taagctgatc ctagatgcac
     5341 aaaaaataaa taaaagtata aacctacttc gtaggatact tcgttttgtt cggggttaga
     5401 tgagcataac gcttgtagtt gatatttgag atcccctatc attgcagggt gacagcggag
     5461 cggcttcgca gagctgcatt aaccagggct tcgggcaggc caaaaactac ggcacgctcc
     5521 tgccacccag tccgccggag gactccggtt cagggagcgg ccaactagcc gagaacctca
     5581 cctatgcctg gcacaatatg gacatctttg gggcggtcaa tcagccgggc tccggatggc
     5641 ggcagctggt caaccggaca cgcggactat tctgcaacga gcgacacata ccggcgccca
     5701 ggaaacattt gctcaagaac ggtgagtttc tattcgcagt cggctgatct gtgtgaaatc
     5761 ttaataaagg gtccaattac caatttgaaa ctcagtttgc ggcgtggcct atccgggcga
     5821 acttttggcc gtgatgggca gttccggtgc cggaaagacg accctgctga atgcccttgc
     5881 ctttcgatcg ccgcagggca tccaagtatc gccatccggg atgcgactgc tcaatggcca
     5941 acctgtggac gccaaggaga tgcaggccag gtgcgcctat gtccagcagg atgacctctt
     6001 tatcggctcc ctaacggcca gggaacacct gattttccag gccatggtgc ggatgccacg
     6061 acatctgacc tatcggcagc gagtggcccg cgtggatcag gtgatccagg agctttcgct
     6121 cagcaaatgt cagcacacga tcatcggtgt gcccggcagg gtgaaaggtc tgtccggcgg
     6181 agaaaggaag cgtctggcat tcgcctccga ggcactaacc gatccgccgc ttctgatctg
     6241 cgatgagccc acctccggac tggactcatt taccgcccac agcgtcgtcc aggtgctgaa
     6301 gaagctgtcg cagaagggca agaccgtcat cctgaccatt catcagccgt cttccgagct
     6361 gtttgagctc tttgacaaga tccttctgat ggccgagggc agggtagctt tcttgggcac
     6421 tcccagcgaa gccgtcgact tcttttccta gtgagttcga tgtgtttatt aagggtatct
     6481 agcattacat tacatctcaa ctcctatcca gcgtgggtgc ccagtgtcct accaactaca
     6541 atccggcgga cttttacgta caggtgttgg ccgttgtgcc cggacgggag atcgagtccc
     6601 gtgatcggat cgccaagata tgcgacaatt ttgctattag caaagtagcc cgggatatgg
     6661 agcagttgtt ggccaccaaa aatttggaga agccactgga gcagccggag aatgggtaca
     6721 cctacaaggc cacctggttc atgcagttcc gggcggtcct gtggcgatcc tggctgtcgg
     6781 tgctcaagga accactcctc gtaaaagtgc gacttattca gacaacggtg agtggttcca
     6841 gtggaaacaa atgatataac gcttacaatt cttggaaaca aattcgctag attttagtta
     6901 gaattgcctg attccacacc cttcttagtt tttttcaatg agatgtatag tttatagttt
     6961 tgcagaaaat aaataaattt catttaactc gcgaacatgt tgaagatatg aatattaatg
     7021 agatgcgagt aacattttaa tttgcagatg gttgccatct tgattggcct catctttttg
     7081 ggccaacaac tcacgcaagt gggcgtgatg aatatcaacg gagccatctt cctcttcctg
     7141 accaacatga cctttcaaaa cgtctttgcc acgataaatg taagtcttgt ttagaataca
     7201 tttgcatatt aataatttac taactttcta atgaatcgat tcgatttagg tgttcacctc
     7261 agagctgcca gtttttatga gggaggcccg aagtcgactt tatcgctgtg acacatactt
     7321 tctgggcaaa acgattgccg aattaccgct ttttctcaca gtgccactgg tcttcacggc
     7381 gattgcctat ccgatgatcg gactgcgggc cggagtgctg cacttcttca actgcctggc
     7441 gctggtcact ctggtggcca atgtgtcaac gtccttcgga tatctaatat cctgcgccag
     7501 ctcctcgacc tcgatggcgc tgtctgtggg tccgccggtt atcataccat tcctgctctt
     7561 tggcggcttc ttcttgaact cgggctcggt gccagtatac ctcaaatggt tgtcgtacct
     7621 ctcatggttc cgttacgcca acgagggtct gctgattaac caatgggcgg acgtggagcc
     7681 gggcgaaatt agctgcacat cgtcgaacac cacgtgcccc agttcgggca aggtcatcct
     7741 ggagacgctt aacttctccg ccgccgatct gccgctggac tacgtgggtc tggccattct
     7801 catcgtgagc ttccgggtgc tcgcatatct ggctctaaga cttcgggccc gacgcaagga
     7861 gtagccgaca tatatccgaa ataactgctt gttttttttt ttaccattat taccatcgtg
     7921 tttactgttt attgccccct caaaaagcta atgtaattat atttgtgcca ataaaaacaa
     7981 gatatgacct atagaataca agtatttccc cttcgaacat ccccacaagt agactttgga
     8041 tttgtcttct aaccaaaaga cttacacacc tgcatacctt acatcaaaaa ctcgtttatc
     8101 gctacataaa acaccgggat atatttttta tatacatact tttcaaatcg cgcgccctct
     8161 tcataattca cctccaccac accacgtttc gtagttgctc tttcgctgtc tcccacccgc
     8221 tctccgcaac acattcacct tttgttcgac gaccttggag cgactgtcgt tagttccgcg
     8281 cgattcggtt cgctcaaatg gttccgagtg gttcatttcg tctcaataga aattagtaat
     8341 aaatatttgt atgtacaatt tatttgctcc aatatatttg tatatatttc cctcacagct
     8401 atatttattc taatttaata ttatgacttt ttaaggtaat tttttgtgac ctgttcggag
     8461 tgattagcgt tacaatttga actgaaagtg acatccagtg tttgttcctt gtgtagatgc
     8521 atctcaaaaa aatggtgggc ataatagtgt tgtttatata tatcaaaaat aacaactata
     8581 ataataagaa tacatttaat ttagaaaatg cttggatttc actggaacta gaattaattc
     8641 ggctgctgct ctaaacgacg catttcgtac tccaaagtac gaattttttc cctcaagctc
     8701 ttattttcat taaacaatga acaggaccta acgcacagtc acgttattgt ttacataaat
     8761 gatttttttt actattcaaa cttactctgt ttgtgtactc ccactggtat agccttcttt
     8821 tatcttttct ggttcaggct ctatcacttt actaggtacg gcatctgcgt tgagtcgcct
     8881 ccttttaaat gtctgacctt ttgcaggtgc agccttccac tgcgaatcat taaagtgggt
     8941 atcacaaatt tgggagtttt caccaaggct gcacccaagg ctctgctccc acaattttct
     9001 cttaatagca cacttcggca cgtgaattaa ttttactcca gtcacagctt tgcagcaaaa
     9061 tttgcaatat ttcatttttt tttattccac gtaagggtta atgttttcaa aaaaaaattc
     9121 gtccgcacac aacctttcct ctcaacaagc aaacgtgcac tgaatttaag tgtatacttc
     9181 ggtaagcttc ggctatcgac gggaccacct tatgttattt catcatg
//
