LOCUS       sequence-82588-e         977 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express U5-40K-LucC fusion protein in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 977)
  AUTHORS   Maita H, Tomita K, Ariga H
  TITLE     A split luciferase-based reporter for detection of a cellular 
            macromolecular complex.
  JOURNAL   Anal Biochem. 2014 Feb 3. pii: S0003-2697(14)00035-9. doi: 
            10.1016/j.ab.2014.01.015.
  PUBMED    24503441
REFERENCE   2  (bases 1 to 977)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Anal 
            Biochem. 2014 Feb 3. pii: S0003-2697(14)00035-9. doi: 
            10.1016/j.ab.2014.01.015."
COMMENT     Sequence Label: pSplit-OC-U5-40K
FEATURES             Location/Qualifiers
     source          1..977
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     52..71
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        52..70
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             87..110
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 cagagctctc tggctaacta gagaacccac tgcttactgg cttatcgaaa ttaatacgac
       61 tcactatagg gagacccaag cttatggact acaaggacga cgatgacaag gaactagctc
      121 tagttgaatt gatgatagaa cagcagaagc gtaagggccc agagttgccg ctggttccag
      181 tcaagcggca gcggcatgag ttgctgttgg gagcggggtc tggcccagga gccgggcagc
      241 agcaggcgac gccgggagcc ttgctgcaag cgggacctcc aagatgttcc tcccttcaag
      301 ccccaatcat gctgctctct ggacatgaag gggaagtcta ctgctgcaag ttccacccca
      361 acggatccac cttagcatct gcaggatttg accgactgat attactgtgg aatgtctatg
      421 gtgactgtga taactatgcc acactgaagg gacacagtgg agcagtgatg gaattgcatt
      481 acaacacaga tggcagtatg cttttctcag catccacaga taaaaccgtg gctgtgtggg
      541 atagtgaaac aggtgagagg gttaaaaggc taaagggaca tacttccttt gtgaattcct
      601 gttatccagc caggagaggc cctcagcttg tctgcactgg cagtgacgat ggcacagtta
      661 agctttggga catccggaag aaagcagcca tccagacatt tcagaacacg taccaggtgt
      721 tagctgtgac cttcaatgac acaagtgatc agattatttc tggtggaata gacaatgata
      781 tcaaggtctg ggacctgcgc cagaacaagc taacctacac catgagaggc catgcagatt
      841 cagtgactgg cctgagttta agttctgaag gctcttatct tttgtccaat gcaatggaca
      901 atacagttcg tgtctgggat gtccggccat ttgcccccaa agagagatgt gtaaagatat
      961 ttcaaggaaa tgtgcac
//
