LOCUS       sequence-82589-e        1051 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express U5-40K-LucC fusion protein in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Maita H, Tomita K, Ariga H
  TITLE     A split luciferase-based reporter for detection of a cellular 
            macromolecular complex.
  JOURNAL   Anal Biochem. 2014 Feb 3. pii: S0003-2697(14)00035-9. doi: 
            10.1016/j.ab.2014.01.015.
  PUBMED    24503441
REFERENCE   2  (bases 1 to 1051)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Anal 
            Biochem. 2014 Feb 3. pii: S0003-2697(14)00035-9. doi: 
            10.1016/j.ab.2014.01.015."
COMMENT     Sequence Label: pSplit-OC-U5-40K
FEATURES             Location/Qualifiers
     source          1..1051
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             518..544
                     /codon_start=1
                     /product="9xHis affinity tag"
                     /label=9xHis
                     /translation="HHHHYHHHH"
ORIGIN
        1 ctatagaata gggccctcta gatgcatgct cgatcgagtt acaatttgga ctttccgccc
       61 ttcttggcct ttatgaggat ctctctgatt tttcttgcgt cgagttttcc ggtaagacct
      121 ttcggtactt cgtccacaaa cacaactcct ccgcgcaact ttttcgcggt tgttacttga
      181 ctggcgacgt aatccacgat ctctttttcc gtcatcgtct ttccgtgctc caaaacaaca
      241 acggcggcgg gaagttcacc ggcgtcatcg tcgggaagac ctgccacgcc cgcgtcgaag
      301 atgttggggt gttgtaacaa tatcgattcc aattcagcgg gggccacctg atatcctttg
      361 tatttaatta aagacttcaa gcggtcaact atgaagaagt gttcgtcttc gtcccagtaa
      421 gctatgtctc cagaatgtag ccatccatcc ttgtcaatca aggcgttggt cgcttccgga
      481 ttgtttacat aaccggacat aatcataggt ccggatccac caccaccact accaccacca
      541 ccaccggtac cctcgagctg aatctctccc atatacagtc tcttgtcact cgatgctgag
      601 ataatgatgg gctcatcagg gtggaaagcc acttcattga tggagccagc atggccgggc
      661 agcttataca atattctcct gcttgtggta tcccacacat aaacaaacct gtcggctgag
      721 ccagctgcta ttttgcttcc atcaggtgac caagaacatc tcagaaggtt cttttcaaag
      781 ttgtgcacat ttccttgaaa tatctttaca catctctctt tgggggcaaa tggccggaca
      841 tcccagacac gaactgtatt gtccattgca ttggacaaaa gataagagcc ttcagaactt
      901 aaactcaggc cagtcactga atctgcatgg cctctcatgg tgtaggttag cttgttctgg
      961 cgcaggtccc agaccttgat atcattgtct attccaccag aaataatctg atcacttgtg
     1021 tcattgaagg tcacagctaa cacctggtac g
//
