LOCUS       sequence-8259-ex        1851 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1851)
  AUTHORS   Rogers SL, Wiedemann U, Stuurman N, Vale RD
  TITLE     Molecular requirements for actin-based lamella formation in 
            Drosophila S2 cells.
  JOURNAL   J Cell Biol. 2003 Sep 15. 162(6):1079-88.
  PUBMED    12975351
REFERENCE   2  (bases 1 to 1851)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2003 Sep 15. 162(6):1079-88."
COMMENT     Sequence Label: pMT-EGFP-Actin5c
FEATURES             Location/Qualifiers
     source          1..1851
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(1..3,4..6,7..720)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1..3,4..6,7..717)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(46..67)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(307..326)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     654..675
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
       61 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
      121 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
      181 ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag
      241 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
      301 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
      361 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
      421 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac
      481 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc
      541 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
      601 tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc
      661 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa
      721 atgtgtgacg aagaagttgc tgctctggtt gtcgacaacg gctctggcat gtgcaaggcc
      781 ggatttgccg gagacgatgc tccccgcgcc gtcttcccat cgattgtggg acgtccccgt
      841 caccagggtg tgatggtcgg catgggccag aaggactcgt acgtgggtga tgaggcgcag
      901 agcaagcgtg gtatcctcac cctgaagtac cccattgagc acggtatcgt gaccaactgg
      961 gacgatatgg agaagatctg gcaccacacc ttctacaatg agctgcgtgt ggcacccgag
     1021 gagcaccccg tgctgctgac cgaggccccg ctgaacccca aggccaaccg tgagaagatg
     1081 acccagatca tgttcgagac cttcaacaca cccgccatgt atgtggccat ccaggctgtg
     1141 ctctcgctgt acgcttcggg tcgtaccacc ggtatcgttc tggactccgg cgatggtgtc
     1201 tcccacaccg tgcccatcta cgagggttat gccctccccc atgccatcct gcgtctggat
     1261 ctggctggtc gcgatttgac cgactacctg atgaagatcc tgaccgagcg cggttactct
     1321 ttcaccacca ccgctgagcg tgaaatcgtc cgtgacatca aggagaagct gtgctatgtt
     1381 gccctcgact ttgagcagga gatggccacc gctgccagca gctcctcgtt ggagaagtcc
     1441 tacgagctgc ccgacggaca ggtgatcacc atcggcaacg agcgtttccg ctgccccgag
     1501 gccctgttcc atccctcgtt ccttgggatg gagtcttgcg gcatccacga gaccacctac
     1561 aactccatca tgaagtgtga tgtggatatc cgtaaggatc tgtatgccaa caccgtgctg
     1621 tccggtggca ccaccatgta ccctggcatc gccgaccgta tgcagaagga gatcaccgcc
     1681 ctggcaccgt cgaccatgaa gatcaagatc attgccccgc cagagcgcaa gtactctgtc
     1741 tggatcggtg gctccatcct ggcttcgtcg tccaccttcc agcagatgtg gacgtccaag
     1801 caggagtacg acgagtccgg cccctccatt gtgcaccgca agtgcttcta a
//
