LOCUS       sequence-82590-e        6415 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Ae aegypti hsp70Bb promoter driving firefly luciferase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6415)
  AUTHORS   Gross TL, Myles KM, Adelman ZN
  TITLE     Identification and characterization of heat shock 70 genes in Aedes 
            aegypti (Diptera: Culicidae).
  JOURNAL   J Med Entomol. 2009 May;46(3):496-504.
  PUBMED    19496419
REFERENCE   2  (bases 1 to 6415)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Med 
            Entomol."; date: "2009-05"; volume: "46(3)"; pages: "496-504"
COMMENT     Sequence Label: AaHsp70Bb-1696-FFluc
FEATURES             Location/Qualifiers
     source          1..6415
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(189..293)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      320..775
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(407..426)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     617..638
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    906..954
                     /note="synthetic polyadenylation signal"
     misc_feature    968..1059
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     1008..1027
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     CDS             2725..4377
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(2791..2809)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     polyA_signal    4418..4539
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4429..4448)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4483..4502
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(4813..4830)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4984..5572)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5064..5083)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5743..6415,1..119,120..188))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6366..6385
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
       61 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
      121 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
      181 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
      241 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
      301 ccccgaaaag tgccacctga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
      361 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
      421 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
      481 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
      541 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
      601 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
      661 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
      721 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttgccat
      781 tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta
      841 cgccagccca agctaccatg ataagtaagt aatattaagg tacgggaggt acttggagcg
      901 gccgcaataa aatatcttta ttttcattac atctgtgtgt tggttttttg tgtgaatcga
      961 tagtactaac atacgctctc catcaaaaca aaacgaaaca aaacaaacta gcaaaatagg
     1021 ctgtccccag tgcaagtgca ggtgccagaa catttctcta tcgataggta ccgagctctt
     1081 acgcgactag atttgagtgg catcaaattt tattttacca tttgcagcaa tcccctggta
     1141 gttctctaca taatgaacaa aatattcttt aagtttgttg gcgttcgtct aatctaattg
     1201 tgataaagtc gagaacatac atttacattt tgcttccaac tattatatgt ttcactgcca
     1261 ttgaatcttc gtctgcagac ctaaaaatgt aagagttaat aatcattggt aatagggcga
     1321 gtggatatga taaatttttt ggaagctttt tccttatttt taaaaggaag atatcaaggt
     1381 aaccgatata accaaccgat attaatcaaa ccattaaaaa aacatcggtg aatcacttcg
     1441 aagcagttta cgttggcaaa gtactagaga gatacatatt tccaaaatat gaagctgaac
     1501 gatgaatcaa aacaaacttt acgaaggcgc gcatgagcac gacaataaca acacgcacac
     1561 tcacacatac tagaatgcgg ccgaatcttc ttgatgtttc tgcttgacga aagatgcggt
     1621 cctctttaga ctggttcatt tatcgaatgc tggataatct attgacaaat taagaaaaat
     1681 gaggtaaatt ttatgagaat aattgattgt aacaatgact taatcataaa tcttcgagct
     1741 gtttagtgga acacctataa gtaaatgaaa acccgaattc agtactatac catttaattc
     1801 cataagagtt tgtatccttt gacagatacc cgtatgtcga cctcaactgt aaggccgtct
     1861 tcagtgttgt aaattcgggt tttcatttac ttactcaatt acttaagttt ttcagactct
     1921 gaagatcaac gacagacaat tcaataaaac ttgtattttt tttttttttt gctaaacgta
     1981 actcatactt aataaaattg tcatctaaac caacataaaa cttgtctttt gggctagcaa
     2041 cgactctatc aaataaaaat caatttcaat tttgataata aatctatata ttgccatact
     2101 ttgattacag tatacaaggg tttattcata tatttcataa cgctaaaaat ggtcattttt
     2161 gatacccaca cacccccttg taatgcattt tgcatggata ttttttaaat tttgtatgag
     2221 ctgtaacatc ttaagaacac tcacccaccc ccttcagcgt tatgaaattt gtaaatgggc
     2281 tccaatagtt tcgaatgaag ctcgcaacta tgacactgac tcatcagtta atggtagcct
     2341 gtttccatga accagtctaa tcgaccctac aaacgcacca gcttgtgcgt aaatgtgacc
     2401 cgtcgaaaag tggagacgca tcgaaatcgt tcgagcaatt cgcacacgaa actactcgaa
     2461 cacttgtgga aagcactcga attttctaga tctgctcgtt tccactatat aagcgcccga
     2521 ttcgagcagc tctcgtcata acagttcaaa caagagaacg aatcacaagc aagagcaata
     2581 aagcgccaaa acgcaaatct acgcaagtga aaagcaaatt ttaacgtgaa acaagttaaa
     2641 acagtgaaat aagtgaatac agtgaaaaag tgtattattt cgaatcaaaa agtgttaaac
     2701 atcagtacaa aagcaaaaat gtccatggaa gacgccaaaa acataaagaa aggcccggcg
     2761 ccattctatc cgctggaaga tggaaccgct ggagagcaac tgcataaggc tatgaagaga
     2821 tacgccctgg ttcctggaac aattgctttt acagatgcac atatcgaggt ggacatcact
     2881 tacgctgagt acttcgaaat gtccgttcgg ttggcagaag ctatgaaacg atatgggctg
     2941 aatacaaatc acagaatcgt cgtatgcagt gaaaactctc ttcaattctt tatgccggtg
     3001 ttgggcgcgt tatttatcgg agttgcagtt gcgcccgcga acgacattta taatgaacgt
     3061 gaattgctca acagtatggg catttcgcag cctaccgtgg tgttcgtttc caaaaagggg
     3121 ttgcaaaaaa ttttgaacgt gcaaaaaaag ctcccaatca tccaaaaaat tattatcatg
     3181 gattctaaaa cggattacca gggatttcag tcgatgtaca cgttcgtcac atctcatcta
     3241 cctcccggtt ttaatgaata cgattttgtg ccagagtcct tcgataggga caagacaatt
     3301 gcactgatca tgaactcctc tggatctact ggtctgccta aaggtgtcgc tctgcctcat
     3361 agaactgcct gcgtgagatt ctcgcatgcc agagatccta tttttggcaa tcaaatcatt
     3421 ccggatactg cgattttaag tgttgttcca ttccatcacg gttttggaat gtttactaca
     3481 ctcggatatt tgatatgtgg atttcgagtc gtcttaatgt atagatttga agaagagctg
     3541 tttctgagga gccttcagga ttacaagatt caaagtgcgc tgctggtgcc aaccctattc
     3601 tccttcttcg ccaaaagcac tctgattgac aaatacgatt tatctaattt acacgaaatt
     3661 gcttctggtg gcgctcccct ctctaaggaa gtcggggaag cggttgccaa gaggttccat
     3721 ctgccaggta tcaggcaagg atatgggctc actgagacta catcagctat tctgattaca
     3781 cccgaggggg atgataaacc gggcgcggtc ggtaaagttg ttccattttt tgaagcgaag
     3841 gttgtggatc tggataccgg gaaaacgctg ggcgttaatc aaagaggcga actgtgtgtg
     3901 agaggtccta tgattatgtc cggttatgta aacaatccgg aagcgaccaa cgccttgatt
     3961 gacaaggatg gatggctaca ttctggagac atagcttact gggacgaaga cgaacacttc
     4021 ttcatcgttg accgcctgaa gtctctgatt aagtacaaag gctatcaggt ggctcccgct
     4081 gaattggaat ccatcttgct ccaacacccc aacatcttcg acgcaggtgt cgcaggtctt
     4141 cccgacgatg acgccggtga acttcccgcc gccgttgttg ttttggagca cggaaagacg
     4201 atgacggaaa aagagatcgt ggattacgtc gccagtcaag taacaaccgc gaaaaagttg
     4261 cgcggaggag ttgtgtttgt ggacgaagta ccgaaaggtc ttaccggaaa actcgacgca
     4321 agaaaaatca gagagatcct cataaaggcc aagaagggcg gaaagatcgc cgtgtaattc
     4381 tagagtcggg gcggccggcc gcttcgagca gacatgataa gatacattga tgagtttgga
     4441 caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt
     4501 gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat
     4561 tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta aaacctctac
     4621 aaatgtggta aaatcgataa ggatcgggcg cgccactagt aggcctggat ccgtcgaccg
     4681 atgcccttga gagccttcaa cccagtcagc tccttccggt gggcgcgggg catgactatc
     4741 gtcgccgcac ttatgactgt cttctttatc atgcaactcg taggacaggt gccggcagcg
     4801 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     4861 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     4921 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     4981 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     5041 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     5101 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     5161 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     5221 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     5281 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     5341 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     5401 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     5461 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     5521 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     5581 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     5641 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     5701 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     5761 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     5821 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     5881 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     5941 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     6001 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     6061 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     6121 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     6181 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     6241 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     6301 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     6361 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaa
//
