LOCUS       sequence-82591-e         943 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of GST tagged AT-Hook2-R270X.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Baker SA, Chen L, Wilkins AD, Yu P, Lichtarge O, Zoghbi HY
  TITLE     An AT-hook domain in MeCP2 determines the clinical course of Rett 
            syndrome and related disorders.
  JOURNAL   Cell. 2013 Feb 28;152(5):984-96. doi: 10.1016/j.cell.2013.01.038.
  PUBMED    23452848
REFERENCE   2  (bases 1 to 943)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.01"; journalName: "Cell"; date: "2013-02-28- 
            28"; volume: "152"; issue: "5"; pages: "984-96"
COMMENT     Sequence Label: pGEX-5x3-hMeCP2-delN256-R270X
FEATURES             Location/Qualifiers
     source          1..943
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             10..21
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             367..387
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
     CDS             733..762
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             763..780
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(849..871)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 tcggatctga tcgaaggtcg tgggatcccc aggaattcag ctgaggccga ccctcaggcc
       61 attcccaaga aacggggctg aaagccgggg agtgtggtgg cagccgctgc cgccgaggcc
      121 aaaaagaaag ccgtgaagga gtcttctatc cgatctgtgc aggagaccgt actccccatc
      181 aagaagcgca agacccggga gacggtcagc atcgaggtca aggaagtggt gaagcccctg
      241 ctggtgtcca ccctcggtga gaagagcggg aaaggactga agacctgtaa gagccctggg
      301 cggaaaagca aggagagcag ccccaagggg cgcagcagca gcgcctcctc accccccaag
      361 aaggagcacc accaccatca ccaccactca gagtccccaa aggcccccgt gccactgctc
      421 ccacccctgc ccccacctcc acctgagccc gagagctccg aggaccccac cagcccccct
      481 gagccccagg acttgagcag cagcgtctgc aaagaggaga agatgcccag aggaggctca
      541 ctggagagcg acggctgccc caaggagcca gctaagactc agcccgcggt tgccaccgcc
      601 gccacggccg cagaaaagta caaacaccga ggggagggag agcgcaaaga cattgtttca
      661 tcctccatgc caaggccaaa cagagaggag cctgtggaca gccggacgcc cgtgaccgag
      721 agagttagcg cagaacagaa actgatctct gaagaagacc tgcatcatca ccatcaccac
      781 tgactcgagc ggccgcatcg tgactgactg acgatctgcc tcgcgcgttt cggtgatgac
      841 ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat
      901 gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttg
//
