LOCUS       sequence-82594-e       10575 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses beta subunit for IKs channel in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10575)
  AUTHORS   Sanguinetti MC, Curran ME, Zou A, Shen J, Spector PS, Atkinson DL, 
            Keating MT
  TITLE     Coassembly of K(V)LQT1 and minK (IsK) proteins to form cardiac I(Ks)
            potassium channel.
  JOURNAL   Nature. 1996 Nov 7;384(6604):80-3.
  PUBMED    8900283
REFERENCE   2  (bases 1 to 10575)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature."; 
            date: "1996-11-7"; volume: "384(6604)"; pages: "80-3"
COMMENT     Sequence Label: pCEP4-hKCNE1
FEATURES             Location/Qualifiers
     source          1..10575
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        4..383
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        384..587
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     538..558
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     584..608
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     polyA_signal    1074..1195
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1085..1104)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1139..1158
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(1338..1360)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      1918..3707
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     CDS             complement(4009..5934)
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     primer_bind     complement(6369..6387)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6455..6559
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6560..6628,6629..7420)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6778..6797)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7591..8179
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8080..8099
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        8610..8755
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             8798..9835
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(9833..9852)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    9877..9925
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtccgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttac gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atggtgatgc ggttttggca gtacaccaat gggcgtggat
      421 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
      481 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa taaccccgcc ccgttgacgc
      541 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc
      601 gtcagatctc tagaagctgg gtaccagctg ctagcagctt aatgcccagg atgatcctgt
      661 ctaacaccac agcggtgacg ccctttctga ccaagctgtg gcaggagaca gttcagcagg
      721 gtggcaacat gtcgggcctg gcccgcaggt ccccccgcag cagtgacggc aagctggagg
      781 ccctctacgt cctcatggta ctgggattct tcggcttctt caccctgggc atcatgctga
      841 gctacatccg ctccaagaag ctggagcact cgaacgaccc attcaacgtc tacatcgagt
      901 ccgatgcctg gcaagagaag gacaaggcct atgtccaggc ccgggtcctg gagagctaca
      961 ggtcgtgcta tgtcgttgaa aaccatttgg ccatagaaca acccaacaca caccttcctg
     1021 agacgaagcc ttccccatga accccaccac tggctaaact ggatcagaca tgataagata
     1081 cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga
     1141 aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa
     1201 caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag
     1261 caagtaaaac ctctacaaat gtggtatggc tgattatgat ccggctgcct cgcgcgtttc
     1321 ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg
     1381 taagcggatg ccgggagcag acaagcccgt caggcgtcag cgggtgttgg cgggtgtcgg
     1441 ggcgcagcca tgaggtcgac tctagaggat cgatgccccg ccccggacga actaaacctg
     1501 actacgacat ctctgcccct tcttcgcggg gcagtgcatg taatcccttc agttggttgg
     1561 tacaacttgc caactgggcc ctgttccaca tgtgacacgg ggggggacca aacacaaagg
     1621 ggttctctga ctgtagttga catccttata aatggatgtg cacatttgcc aacactgagt
     1681 ggctttcatc ctggagcaga ctttgcagtc tgtggactgc aacacaacat tgcctttatg
     1741 tgtaactctt ggctgaagct cttacaccaa tgctggggga catgtacctc ccaggggccc
     1801 aggaagacta cgggaggcta caccaacgtc aatcagaggg gcctgtgtag ctaccgataa
     1861 gcggaccctc aagagggcat tagcaatagt gtttataagg cccccttgtt aaccctaaac
     1921 gggtagcata tgcttcccgg gtagtagtat atactatcca gactaaccct aattcaatag
     1981 catatgttac ccaacgggaa gcatatgcta tcgaattagg gttagtaaaa gggtcctaag
     2041 gaacagcgat atctcccacc ccatgagctg tcacggtttt atttacatgg ggtcaggatt
     2101 ccacgagggt agtgaaccat tttagtcaca agggcagtgg ctgaagatca aggagcgggc
     2161 agtgaactct cctgaatctt cgcctgcttc ttcattctcc ttcgtttagc taatagaata
     2221 actgctgagt tgtgaacagt aaggtgtatg tgaggtgctc gaaaacaagg tttcaggtga
     2281 cgcccccaga ataaaatttg gacggggggt tcagtggtgg cattgtgcta tgacaccaat
     2341 ataaccctca caaacccctt gggcaataaa tactagtgta ggaatgaaac attctgaata
     2401 tctttaacaa tagaaatcca tggggtgggg acaagccgta aagactggat gtccatctca
     2461 cacgaattta tggctatggg caacacataa tcctagtgca atatgatact ggggttatta
     2521 agatgtgtcc caggcaggga ccaagacagg tgaaccatgt tgttacactc tatttgtaac
     2581 aaggggaaag agagtggacg ccgacagcag cggactccac tggttgtctc taacaccccc
     2641 gaaaattaaa cggggctcca cgccaatggg gcccataaac aaagacaagt ggccactctt
     2701 ttttttgaaa ttgtggagtg ggggcacgcg tcagccccca cacgccgccc tgcggttttg
     2761 gactgtaaaa taagggtgta ataacttggc tgattgtaac cccgctaacc actgcggtca
     2821 aaccacttgc ccacaaaacc actaatggca ccccggggaa tacctgcata agtaggtggg
     2881 cgggccaaga taggggcgcg attgctgcga tctggaggac aaattacaca cacttgcgcc
     2941 tgagcgccaa gcacagggtt gttggtcctc atattcacga ggtcgctgag agcacggtgg
     3001 gctaatgttg ccatgggtag catatactac ccaaatatct ggatagcata tgctatccta
     3061 atctatatct gggtagcata ggctatccta atctatatct gggtagcata tgctatccta
     3121 atctatatct gggtagtata tgctatccta atttatatct gggtagcata ggctatccta
     3181 atctatatct gggtagcata tgctatccta atctatatct gggtagtata tgctatccta
     3241 atctgtatcc gggtagcata tgctatccta atagagatta gggtagtata tgctatccta
     3301 atttatatct gggtagcata tactacccaa atatctggat agcatatgct atcctaatct
     3361 atatctgggt agcatatgct atcctaatct atatctgggt agcataggct atcctaatct
     3421 atatctgggt agcatatgct atcctaatct atatctgggt agtatatgct atcctaattt
     3481 atatctgggt agcataggct atcctaatct atatctgggt agcatatgct atcctaatct
     3541 atatctgggt agtatatgct atcctaatct gtatccgggt agcatatgct atcctcatgc
     3601 atatacagtc agcatatgat acccagtagt agagtgggag tgctatcctt tgcatatgcc
     3661 gccacctccc aagggggcgt gaattttcgc tgcttgtcct tttcctgctg gttgctccca
     3721 ttcttaggtg aatttaagga ggccaggcta aagccgtcgc atgtctgatt gctcaccagg
     3781 taaatgtcgc taatgttttc caacgcgaga aggtgttgag cgcggagctg agtgacgtga
     3841 caacatgggt atgcccaatt gccccatgtt gggaggacga aaatggtgac aagacagatg
     3901 gccagaaata caccaacagc acgcatgatg tctactgggg atttattctt tagtgcgggg
     3961 gaatacacgg cttttaatac gattgagggc gtctcctaac aagttacatc actcctgccc
     4021 ttcctcaccc tcatctccat cacctccttc atctccgtca tctccgtcat caccctccgc
     4081 ggcagcccct tccaccatag gtggaaacca gggaggcaaa tctactccat cgtcaaagct
     4141 gcacacagtc accctgatat tgcaggtagg agcgggcttt gtcataacaa ggtccttaat
     4201 cgcatccttc aaaacctcag caaatatatg agtttgtaaa aagaccatga aataacagac
     4261 aatggactcc cttagcgggc caggttgtgg gccgggtcca ggggccattc caaaggggag
     4321 acgactcaat ggtgtaagac gacattgtgg aatagcaagg gcagttcctc gccttaggtt
     4381 gtaaagggag gtcttactac ctccatatac gaacacaccg gcgacccaag ttccttcgtc
     4441 ggtagtcctt tctacgtgac tcctagccag gagagctctt aaaccttctg caatgttctc
     4501 aaatttcggg ttggaacctc cttgaccacg atgctttcca aaccaccctc cttttttgcg
     4561 cctgcctcca tcaccctgac cccggggtcc agtgcttggg ccttctcctg ggtcatctgc
     4621 ggggccctgc tctatcgctc ccgggggcac gtcaggctca ccatctgggc caccttcttg
     4681 gtggtattca aaataatcgg cttcccctac agggtggaaa aatggccttc tacctggagg
     4741 gggcctgcgc ggtggagacc cggatgatga tgactgacta ctgggactcc tgggcctctt
     4801 ttctccacgt ccacgacctc tccccctggc tctttcacga cttccccccc tggctctttc
     4861 acgtcctcta ccccggcggc ctccactacc tcctcgaccc cggcctccac tacctcctcg
     4921 accccggcct ccactgcctc ctcgaccccg gcctccacct cctgctcctg cccctcctgc
     4981 tcctgcccct cctcctgctc ctgcccctcc tgcccctcct gctcctgccc ctcctgcccc
     5041 tcctgctcct gcccctcctg cccctcctgc tcctgcccct cctgcccctc ctcctgctcc
     5101 tgcccctcct gcccctcctc ctgctcctgc ccctcctgcc cctcctgctc ctgcccctcc
     5161 tgcccctcct gctcctgccc ctcctgcccc tcctgctcct gcccctcctg ctcctgcccc
     5221 tcctgctcct gcccctcctg ctcctgcccc tcctgcccct cctgcccctc ctcctgctcc
     5281 tgcccctcct gctcctgccc ctcctgcccc tcctgcccct cctgctcctg cccctcctcc
     5341 tgctcctgcc cctcctgccc ctcctgcccc tcctcctgct cctgcccctc ctgcccctcc
     5401 tcctgctcct gcccctcctc ctgctcctgc ccctcctgcc cctcctgccc ctcctcctgc
     5461 tcctgcccct cctgcccctc ctcctgctcc tgcccctcct cctgctcctg cccctcctgc
     5521 ccctcctgcc cctcctcctg ctcctgcccc tcctcctgct cctgcccctc ctgcccctcc
     5581 tgcccctcct gcccctcctc ctgctcctgc ccctcctcct gctcctgccc ctcctgctcc
     5641 tgcccctccc gctcctgctc ctgctcctgt tccaccgtgg gtccctttgc agccaatgca
     5701 acttggacgt ttttggggtc tccggacacc atctctatgt cttggccctg atcctgagcc
     5761 gcccggggct cctggtcttc cgcctcctcg tcctcgtcct cttccccgtc ctcgtccatg
     5821 gttatcaccc cctcttcttt gaggtccact gccgccggag ccttctggtc cagatgtgtc
     5881 tcccttctct cctaggccat ttccaggtcc tgtacctggc ccctcgtcag acatgattca
     5941 cactaaaaga gatcaataga catctttatt agacgacgct cagtgaatac agggagtgca
     6001 gactcctgcc ccctccaaca gcccccccac cctcatcccc ttcatggtcg ctgtcagaca
     6061 gatccaggtc tgaaaattcc ccatcctccg aaccatcctc gtcctcatca ccaattactc
     6121 gcagcccgga aaactcccgc tgaacatcct caagatttgc gtcctgagcc tcaagccagg
     6181 cctcaaattc ctcgtccccc tttttgctgg acggtaggga tggggattct cgggacccct
     6241 cctcttcctc ttcaaggtca ccagacagag atgctactgg ggcaacggaa gaaaagctgg
     6301 gtgcggcctg tgaggatcag cttatcgatg ataagctgtc aaacatgaga attcttgaag
     6361 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
     6421 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     6481 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     6541 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     6601 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     6661 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     6721 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     6781 atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca
     6841 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     6901 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     6961 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     7021 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     7081 cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg
     7141 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     7201 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     7261 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     7321 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     7381 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     7441 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     7501 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     7561 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     7621 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     7681 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
     7741 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     7801 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     7861 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     7921 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     7981 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     8041 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     8101 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     8161 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     8221 ctggccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa
     8281 cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc
     8341 tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccccgagat gcgccgcgtg
     8401 cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc
     8461 acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg
     8521 tgccgccctg cttcatcccc gtggcccgtt gctcgcgttt gctggcggtg tccccggaag
     8581 aaatatattt gcatgtcttt agttctatga tgacacaaac cccgcccagc gtcttgtcat
     8641 tggcgaattc gaacacgcag atgcagtcgg ggcggcgcgg tccgaggtcc acttcgcata
     8701 ttaaggtgac gcgtgtggcc tcgaacaccg agcgaccctg cagcgacccg cttaacagcg
     8761 tcaacagcgt gccgcagatc ccggggggca atgagatatg aaaaagcctg aactcaccgc
     8821 gacgtctgtc gagaagtttc tgatcgaaaa gttcgacagc gtctccgacc tgatgcagct
     8881 ctcggagggc gaagaatctc gtgctttcag cttcgatgta ggagggcgtg gatatgtcct
     8941 gcgggtaaat agctgcgccg atggtttcta caaagatcgt tatgtttatc ggcactttgc
     9001 atcggccgcg ctcccgattc cggaagtgct tgacattggg gaattcagcg agagcctgac
     9061 ctattgcatc tcccgccgtg cacagggtgt cacgttgcaa gacctgcctg aaaccgaact
     9121 gcccgctgtt ctgcagccgg tcgcggaggc catggatgcg atcgctgcgg ccgatcttag
     9181 ccagacgagc gggttcggcc cattcggacc gcaaggaatc ggtcaataca ctacatggcg
     9241 tgatttcata tgcgcgattg ctgatcccca tgtgtatcac tggcaaactg tgatggacga
     9301 caccgtcagt gcgtccgtcg cgcaggctct cgatgagctg atgctttggg ccgaggactg
     9361 ccccgaagtc cggcacctcg tgcacgcgga tttcggctcc aacaatgtcc tgacggacaa
     9421 tggccgcata acagcggtca ttgactggag cgaggcgatg ttcggggatt cccaatacga
     9481 ggtcgccaac atcttcttct ggaggccgtg gttggcttgt atggagcagc agacgcgcta
     9541 cttcgagcgg aggcatccgg agcttgcagg atcgccgcgg ctccgggcgt atatgctccg
     9601 cattggtctt gaccaactct atcagagctt ggttgacggc aatttcgatg atgcagcttg
     9661 ggcgcagggt cgatgcgacg caatcgtccg atccggagcc gggactgtcg ggcgtacaca
     9721 aatcgcccgc agaagcgcgg ccgtctggac cgatggctgt gtagaagtac tcgccgatag
     9781 tggaaaccga cgccccagca ctcgtccgga tcgggagatg ggggaggcta actgaaacac
     9841 ggaaggagac aataccggaa ggaacccgcg ctatgacggc aataaaaaga cagaataaaa
     9901 cgcacgggtg ttgggtcgtt tgttcataaa cgcggggttc ggtcccaggg ctggcactct
     9961 gtcgataccc caccgagacc ccattggggc caatacgccc gcgtttcttc cttttcccca
    10021 ccccaccccc caagttcggg tgaaggccca gggctcgcag ccaacgtcgg ggcggcaggc
    10081 cctgccatag ccactggccc cgtgggttag ggacggggtc ccccatgggg aatggtttat
    10141 ggttcgtggg ggttattatt ttgggcgttg cgtggggtca ggtccacgac tggactgagc
    10201 agacagaccc atggtttttg gatggcctgg gcatggaccg catgtactgg cgcgacacga
    10261 acaccgggcg tctgtggctg ccaaacaccc ccgaccccca aaaaccaccg cgcggatttc
    10321 tggcgtgcca agctagtcga ccaattctca tgtttgacag cttatcatcg cagatccggg
    10381 caacgttgtt gccattgctg caggcgcaga actggtaggt atggaagatc tatacattga
    10441 atcaatattg gcaattagcc atattagtca ttggttatat agcataaatc aatattggct
    10501 attggccatt gcatacgttg tatctatatc ataatatgta catttatatt ggctcatgtc
    10561 caatatgacc gccat
//
