LOCUS       sequence-82595-e        1014 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression vectore in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1014)
  AUTHORS   Yoo SH, Mohawk JA, Siepka SM, Shan Y, Huh SK, Hong HK, Kornblum I, 
            Kumar V, Koike N, Xu M, Nussbaum J, Liu X, Chen Z, Chen ZJ, Green 
            CB, Takahashi JS
  TITLE     Competing E3 ubiquitin ligases govern circadian periodicity by 
            degradation of CRY in nucleus and cytoplasm.
  JOURNAL   Cell. 2013 Feb 28;152(5):1091-105. doi: 10.1016/j.cell.2013.01.055.
  PUBMED    23452855
REFERENCE   2  (bases 1 to 1014)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.01"; journalName: "Cell"; date: "2013-02-28- 
            28"; volume: "152"; issue: "5"; pages: "1091-105"
COMMENT     Sequence Label: pCMV10-Flag-Ovtm
FEATURES             Location/Qualifiers
     source          1..1014
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             39..104
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 agcagagctc gtttagtgaa ccgtcagaat taaccatgga ctacaaagac catgacggtg
       61 attataaaga tcatgacatc gattacaagg atgacgatga caagcttgcg gccgcgaatt
      121 catcgataga tctgatatcg gtaccgatga aacgaggagg aagagatagt gaccaggact
      181 ctgctgagga aggcactgcc gagaagccca agagaccgag gacgacacag gagcgctcgc
      241 agccttgtga ctggggtaac ctcctgcagg acatcgtcct ccatgttttt aagtatttgc
      301 ctcttcttga ccgggctcat gcttcccaag tgtgccggaa ttggaatcag gtatttcaca
      361 tgcctgactt gtggcgatgt tttgaatttg aactgaatca gccagcgaca tcttacttga
      421 aagccacaca cccagagctg atcaaacaga ttatcaaaag gcactcaaac cacctacagt
      481 atgtcagctt caaggtggac agcagcaaag aatcagctga agctgcttgt gacatactgt
      541 cgcagcttgt gaattgctct ttaaaaactc tcggacttat ctcgactgcc cggccaagct
      601 ttatggatct accaaagtct cactttatct ctgcactgac agttgtgttt gtaaactcca
      661 agtccctgtc ctcacttaag atagacgaca ccccagtcga tgacccatcc cttaaagtcc
      721 tcgtggccaa caacagcgac acactcaagc tgttgaaaat gagcagctgt cctcacgtct
      781 ctccagcagg cattctgtgt gtggctgacc agtgtcatgg cttacgagaa ctggccctga
      841 actaccattt gctaagtgat gagttgttgc tcgcactgtc ttctgaaaag catgttcgct
      901 tagaacattt gcgcattgat gttgtcagtg agaacccggg acagacacac ttccacacta
      961 tccagaagag cagctgggat gccttcatca aacactcacc gaaagtcaac ctag
//
