LOCUS       sequence-82608-e        1099 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1099)
  AUTHORS   Baughman JM, Perocchi F, Girgis HS, Plovanich M, Belcher-Timme CA, 
            Sancak Y, Bao XR, Strittmatter L, Goldberger O, Bogorad RL, 
            Koteliansky V, Mootha VK
  TITLE     Integrative genomics identifies MCU as an essential component of the
            mitochondrial calcium uniporter.
  JOURNAL   Nature. 2011 Jun 19;476(7360):341-5. doi: 10.1038/nature10234.
  PUBMED    21685886
REFERENCE   2  (bases 1 to 1099)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2011-06-19- 19"; volume: "476"; issue: "7360"; 
            pages: "341-5"
COMMENT     Sequence Label: pDEST47-MCU-GFP
FEATURES             Location/Qualifiers
     source          1..1099
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..80
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    113..137
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
       61 taatacgact cactataggg agacccaagc tggctagtta agctgagcat caacaagttt
      121 gtacaaaaaa gttggcatga cggccgccgc aggtagatcg ctcctgctgc tcctctcctc
      181 tcggggcggc ggcggcgggg gcgccggcgg ctgcggggcg ctgactgccg gctgcttccc
      241 tgggctgggc gtcagccgcc accggcagca gcagcaccac cggacggtac accagaggat
      301 cgcttcctgg cagaatttgg gagctgttta ttgcagcact gttgtgccct ctgatgatgt
      361 tacagtggtt tatcaaaatg ggttacctgt gatatctgtg aggctaccat cccggcgtga
      421 acgctgtcag ttcacactca agcctatctc tgactctgtt ggtgtatttt tacgacaact
      481 gcaagaagag gatcggggaa ttgacagagt tgctatctat tcaccagatg gtgttcgcgt
      541 tgctgcttca acaggaatag acctcctcct ccttgatgac tttaagctgg tcattaatga
      601 cttaacatac cacgtacgac caccaaaaag agacctctta agtcatgaaa atgcagcaac
      661 gctgaatgat gtaaagacat tggtccagca actatacacc acactgtgca ttgagcagca
      721 ccagttaaac aaggaaaggg agcttattga aagactagag gatctcaaag agcagctggc
      781 tcccctggaa aaggtacgaa ttgagattag cagaaaagct gagaagagga ccactttggt
      841 gctatggggt ggccttgcct acatggccac acagtttggc attttggccc ggcttacctg
      901 gtgggaatat tcctgggaca tcatggagcc agtaacatac ttcatcactt atggaagtgc
      961 catggcaatg tatgcatatt ttgtaatgac acgccaggaa tatgtttatc cagaagccag
     1021 agacagacaa tacttactat ttttccataa aggagccaaa aagtcacgtt ttgacctaga
     1081 gaaatacaat caactcaag
//
