LOCUS       sequence-82609-e        1203 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of human ubiquitin with HA tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1203)
  AUTHORS   Kamitani T, Kito K, Nguyen HP, Yeh ET
  TITLE     Characterization of NEDD8, a developmentally down-regulated 
            ubiquitin-like protein.
  JOURNAL   J Biol Chem. 1997 Nov 7. 272(45):28557-62.
  PUBMED    9353319
REFERENCE   2  (bases 1 to 1203)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 1997 Nov 7. 272(45):28557-62."
COMMENT     Sequence Label: HA-Ubiquitin
FEATURES             Location/Qualifiers
     source          1..1203
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..80
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             96..122
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             132..359
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     CDS             132..359
                     /codon_start=1
                     /gene="Homo sapiens UBB"
                     /product="human ubiquitin B"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     promoter        complement(422..440)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(423..440)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(460..477)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    466..690
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      736..1164
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(823..842)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1033..1054
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(1173..1193)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
       61 taatacgact cactataggg agacccaagc ttatgtaccc atacgatgtt ccggattacg
      121 ctagcggatc catgcagatc ttcgtgaaaa cccttaccgg caagaccatc acccttgagg
      181 tggagcccag tgacaccatc gaaaatgtga aggccaagat ccaggataag gaaggcattc
      241 cccccgacca gcagaggctc atctttgcag gcaagcagct ggaagatggc cgtactcttt
      301 ctgactacaa catccagaag gagtcgaccc tgcacctggt cctgcgtctg agaggtggtt
      361 agaattctgc agatatccat cacactggcg gccgctcgag catgcatcta gagggcccta
      421 ttctatagtg tcacctaaat gctagagctc gctgatcagc ctcgactgtg ccttctagtt
      481 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc
      541 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt
      601 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca
      661 ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct
      721 ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
      781 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
      841 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
      901 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
      961 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     1021 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
     1081 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
     1141 tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa
     1201 gtc
//
