LOCUS       sequence-82616-e         912 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Forebrain principal neuron expression of oChIEF kinetic variant 
            E163A/T199C with physically uncoupled EGFP fluorophore.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 912)
  TITLE     ChIEF and oChIEF AAV vectors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 912)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pAAV-CaMKIIa-oChIEF(E163A/T199C)-P2A-EGFP
FEATURES             Location/Qualifiers
     source          1..912
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      224..233
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gcagttaccg gggcaacggg aacagggcgt ttcggaggtg gttgccatgg ggacctggat
       61 gctgacgaag gctcgcgagg ctgtgagcag ccacagtgcc ctgctcagaa gccccaagct
      121 cgtcagtcaa gccggttctc cgtttgcact caggagcacg ggcaggcgag tggcccctag
      181 ttctgggggc agcgggggat ccttcgaaac cggtgctagc ggcgccacca tggtgagcag
      241 aagaccctgg ctgctggccc tggccctggc cgtggccctg gccgccggca gcgccggcgc
      301 cagcaccggc agcgacgcca ccgtgcccgt ggccacccag gacggccccg actacgtgtt
      361 ccacagagcc cacgagagaa tgctgttcca gaccagctac accctggaga acaacggcag
      421 cgtgatctgc atccccaaca acggccagtg cttctgcctg gcctggctga agagtaacgg
      481 caccaacgcc gagaagctgg ccgccaacat cctgcagtgg atcaccttcg ccctgagcgc
      541 cctgtgcctg atgttctacg gctaccagac ctggaagagt acctgcggct gggaggagat
      601 ctacgtggcc accatcgaga tgatcaagtt catcatagag tacttccacg agttcgacga
      661 gcccgccgtg atctacagca gcaacggcaa caagaccgtg tggctgagat acgccgcctg
      721 gctgctgacc tgccccgtgg tcctgatcca cctgagcaac ctgaccggcc tggccaacga
      781 ctacaacaag agaaccatgg gcctgctggt gagcgacatc ggctgtatcg tgtggggcac
      841 caccgccgcc ctgagcaagg gctacgtgag agtgatcttc ttcctgatgg gcctgtgcta
      901 cggcatctac ac
//
