LOCUS       sequence-82621-e         852 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of human SPOP (aa 28-337) fused to His-MBP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Zhuang M, Calabrese MF, Liu J, Waddell MB, Nourse A, Hammel M, 
            Miller DJ, Walden H, Duda DM, Seyedin SN, Hoggard T, Harper JW, 
            White KP, Schulman BA
  TITLE     Structures of SPOP-substrate complexes: insights into molecular 
            architectures of BTB-Cul3 ubiquitin ligases.
  JOURNAL   Mol Cell. 2009 Oct 9;36(1):39-50. doi: 10.1016/j.molcel.2009.09.022.
  PUBMED    19818708
REFERENCE   2  (bases 1 to 852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2009.09"; journalName: "Mol Cell"; date: 
            "2009-10-9- 9"; volume: "36"; issue: "1"; pages: "39-50"
COMMENT     Sequence Label: His-MBP human SPOP (aa 28-337)
FEATURES             Location/Qualifiers
     source          1..852
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             33..53
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 acaataacaa taacaacaac ctcgggatcg aggaaaacct gtattttcag ggatccaaag
       61 tagtgaaatt ctcctacatg tggaccatca ataactttag cttttgccgg gaggaaatgg
      121 gtgaagtcat taaaagttct acattttcat caggagcaaa tgataaactg aaatggtgtt
      181 tgcgagtaaa ccccaaaggg ttagatgaag aaagcaaaga ctacctgtca ctttacctgt
      241 tactggtcag ctgtccaaag agtgaagttc gggcaaaatt caaattctcc atcctgaatg
      301 ccaagggaga agaaaccaaa gctatggaga gtcaacgggc atataggttt gtgcaaggca
      361 aagactgggg attcaagaaa ttcatccgta gagattttct tttggatgag gccaacgggc
      421 ttctccctga tgacaagctt accctcttct gcgaggtgag tgttgtgcaa gattctgtca
      481 acatttctgg ccagaatacc atgaacatgg taaaggttcc tgagtgccgg ctggcagatg
      541 agttaggagg actgtgggag aattcccggt tcacagactg ctgcttgtgt gttgccggcc
      601 aggaattcca ggctcacaag gctatcttag cagctcgttc tccggttttt agtgccatgt
      661 ttgaacatga aatggaggag agcaaaaaga atcgagttga aatcaatgat gtggagcctg
      721 aagtttttaa ggaaatgatg tgcttcattt acacggggaa ggctccaaac ctcgacaaaa
      781 tggctgatga tttgctggca gctgctgaca agtatgccct ggagcgctta aaggtcatgt
      841 gtgaggatgc cc
//
