LOCUS       sequence-82631-e         717 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral expression vector for TAZ with N-terminal FLAG and His 
            tags.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 717)
  AUTHORS   Yang Z, Nakagawa K, Sarkar A, Maruyama J, Iwasa H, Bao Y, 
            Ishigami-Yuasa M, Ito S, Kagechika H, Hata S, Nishina H, Abe S, 
            Kitagawa M, Hata Y
  TITLE     Screening with a novel cell-based assay for TAZ activators 
            identifies a compound that enhances myogenesis in C2C12 cells and 
            facilitates muscle repair in the muscle injury model.
  JOURNAL   Mol Cell Biol. 2014 Feb 18.
  PUBMED    24550007
REFERENCE   2  (bases 1 to 717)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 2014 Feb 18."
COMMENT     Sequence Label: pLenti-EF-FH-TAZ-ires-blast
FEATURES             Location/Qualifiers
     source          1..717
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             36..59
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             66..83
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gaagcttgtt aacgtttaaa ctagccgggg ccatggacta caaagacgat gacgataagg
       61 aattgcacca tcatcatcat catgaattca cgcgctcagg ctcagcttcg ctgcccgccc
      121 agaagatgaa tccggcctcg gcgccccctc cgctcccgcc gcctgggcag caagtgatcc
      181 acgtcacgca ggacctagac acagacctcg aagccctctt caactctgtc atgaatccga
      241 agcctagctc gtggcggaag aagatcctgc cggagtcttt ctttaaggag cctgattcgg
      301 gctcgcactc gcgccagtcc agcaccgact cgtcgggcgg ccacccgggg cctcgactgg
      361 ctgggggtgc ccagcatgtc cgctcgcact cgtcgcccgc gtccctgcag ctgggcaccg
      421 gcgcgggtgc tgcgggtagc cccgcgcagc agcacgcgca cctccgccag cagtcctacg
      481 acgtgaccga cgagctgcca ctgcccccgg gctgggagat gaccttcacg gccactggcc
      541 agaggtactt cctcaatcac atagaaaaaa tcaccacatg gcaagaccct aggaaggcga
      601 tgaatcagcc tctgaatcat atgaacctcc accctgccgt cagttccaca ccagtgcctc
      661 agaggtccat ggcagtatcc cagccaaatc tcgtgatgaa tcaccaacac cagcagc
//
