LOCUS       sequence-82633-e         833 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral RNAi vector for knockdown of LATS2 and LATS1. 
            Co-expresses EmGFP as reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Yang Z, Nakagawa K, Sarkar A, Maruyama J, Iwasa H, Bao Y, 
            Ishigami-Yuasa M, Ito S, Kagechika H, Hata S, Nishina H, Abe S, 
            Kitagawa M, Hata Y
  TITLE     Screening with a novel cell-based assay for TAZ activators 
            identifies a compound that enhances myogenesis in C2C12 cells and 
            facilitates muscle repair in the muscle injury model.
  JOURNAL   Mol Cell Biol. 2014 Feb 18.
  PUBMED    24550007
REFERENCE   2  (bases 1 to 833)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 2014 Feb 18."
COMMENT     Sequence Label: pLenti-EmGFP-LATS2/1 KD
FEATURES             Location/Qualifiers
     source          1..833
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    53..77
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(86..88,89..91,92..805)
                     /codon_start=1
                     /product="Emerald GFP"
                     /label=EmGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(131..152)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(392..411)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     739..760
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gagcttgtta acgtttaaac tagtccagtg tggtggaatt ctgcagatat caacaagttt
       61 gtacaaaaaa gcaggcttta aaaccatggt gagcaagggc gaggagctgt tcaccggggt
      121 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg
      181 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg
      241 caagctgccc gtgccctggc ccaccctcgt gaccaccttc acctacggcg tgcagtgctt
      301 cgcccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg
      361 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga
      421 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa
      481 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaaggtcta
      541 tatcaccgcc gacaagcaga agaacggcat caaggtgaac ttcaagaccc gccacaacat
      601 cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg
      661 ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc
      721 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct
      781 cggcatggac gagctgtaca agtaagctaa gcacttcgtg gccgtcgatc gtt
//
