LOCUS       sequence-82636-e         832 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses a myc-tagged version of hCas9 in Drosophila.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Bottcher R, Hollmann M, Merk K, Nitschko V, Obermaier C, 
            Philippou-Massier J, Wieland I, Gaul U, Forstemann K
  TITLE     Efficient chromosomal gene modification with CRISPR/cas9 and 
            PCR-based homologous recombination donors in cultured Drosophila 
            cells.
  JOURNAL   Nucleic Acids Res. 2014 Apr 19.
  PUBMED    24748663
REFERENCE   2  (bases 1 to 832)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2014 Apr 19."
COMMENT     Sequence Label: pRB14
FEATURES             Location/Qualifiers
     source          1..832
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             252..281
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 ccatgatact tcgacgcata actgtagatt ttggatagaa ttaaagagaa aatggcgaga
       61 gagtaaaatt ccggcgtcgg caaagtagag caaaaaaatc agtataccat ttagctacct
      121 ctctcactcg cacgcagtgc cggctcaagt tgggcgcggc tctgcaatta tcgattttct
      181 tggggtgtgt aactaatcat ccgttttccc ttcctcctca tccacagcgt gaaggtacct
      241 aatccaaaat ggaacagaaa ctgattagcg aagaagacct ggacaagaag tactccattg
      301 ggctcgatat cggcacaaac agcgtcggct gggccgtcat tacggacgag tacaaggtgc
      361 cgagcaaaaa attcaaagtt ctgggcaata ccgatcgcca cagcataaag aagaacctca
      421 ttggcgccct cctgttcgac tccggggaga cggccgaagc cacgcggctc aaaagaacag
      481 cacggcgcag atatacccgc agaaagaatc ggatctgcta cctgcaggag atctttagta
      541 atgagatggc taaggtggat gactctttct tccataggct ggaggagtcc tttttggtgg
      601 aggaggataa aaagcacgag cgccacccaa tctttggcaa tatcgtggac gaggtggcgt
      661 accatgaaaa gtacccaacc atatatcatc tgaggaagaa gcttgtagac agtactgata
      721 aggctgactt gcggttgatc tatctcgcgc tggcgcatat gatcaaattt cggggacact
      781 tcctcatcga gggggacctg aacccagaca acagcgatgt cgacaaactc tt
//
