LOCUS       sequence-82637-e         907 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  serves as template for PCR-mediated fusion of U6-promoter with 
            sgRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 907)
  AUTHORS   Bottcher R, Hollmann M, Merk K, Nitschko V, Obermaier C, 
            Philippou-Massier J, Wieland I, Gaul U, Forstemann K
  TITLE     Efficient chromosomal gene modification with CRISPR/cas9 and 
            PCR-based homologous recombination donors in cultured Drosophila 
            cells.
  JOURNAL   Nucleic Acids Res. 2014 Apr 19.
  PUBMED    24748663
REFERENCE   2  (bases 1 to 907)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2014 Apr 19."
COMMENT     Sequence Label: pRB17
FEATURES             Location/Qualifiers
     source          1..907
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gtttttcagc aagatgctca cctgtgattg ctcctactca aatacaaaaa catcaaattt
       61 tctgtcaata aagcatattt atttatattt attttacagg aaagaattcc ttttaaagtg
      121 tattttaacc tataatgaaa aacgattaaa aaaaatacat aaaataattc gaaaattttt
      181 gaatagccca ggttgataaa aattcatttc atacgtttta taacttatgc ccctaagtat
      241 tttttgacca tagtgtttca attctacatt aattttacag agtagaatga aacgccacct
      301 actcagccaa gaggcgaaaa ggttagctcg ccaagcagag agggcgccag tgctcactac
      361 tttttataat tctcaacttc tttttccaga ctcagttcgt atatatagac ctattttcaa
      421 tttaacgtcg tttaatacga ctcactatat ctttctagaa gatctcctac aatattctca
      481 gctgccatgg aaaatcgatg ttcttctttt attctctcaa gattttcagg ctgtatatta
      541 aaacttatat taagaactat gctaaccacc tcatcaggaa ccgttgtagg tggcgtgggt
      601 tttcttggca atcgactctc atgaaaacta cgagctaaat attcaatatg ttcctcttga
      661 ccaactttat tctgcatttt ttttgaacga ggtttagagc aagcttcagg aaactgagac
      721 aggaatttta ttaaaaattt aaattttgaa gaaagttcag ggttaatagc atccattttt
      781 tgctttgcaa gttcctcagc attcttaaca aaagacgtct cttttgacat gtttaaagtt
      841 taaacctcct gtgtgaaatt attatccgct cataattcca cacattatac gagccggaag
      901 cataaag
//
