LOCUS       sequence-82639-e         923 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  serves as PCR-template to generate HR donors for C-terminal 
            2xFLAG-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 923)
  AUTHORS   Bottcher R, Hollmann M, Merk K, Nitschko V, Obermaier C, 
            Philippou-Massier J, Wieland I, Gaul U, Forstemann K
  TITLE     Efficient chromosomal gene modification with CRISPR/cas9 and 
            PCR-based homologous recombination donors in cultured Drosophila 
            cells.
  JOURNAL   Nucleic Acids Res. 2014 Apr 19.
  PUBMED    24748663
REFERENCE   2  (bases 1 to 923)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2014 Apr 19."
COMMENT     Sequence Label: pMH4
FEATURES             Location/Qualifiers
     source          1..923
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             9..32
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             42..65
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    complement(75..108)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     CDS             385..783
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     protein_bind    complement(832..865)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 ggctcgagga ttataaagat gatgatgata aatccggagc cgattataaa gatgatgatg
       61 ataaatgaac tagtgaagtt cctatacttt ctagagaata ggaacttcaa ataaacatat
      121 gctgttggaa tatactattc aacctacaaa aataacgtta aacaacacta ctttatattt
      181 gatatgaatg gccacacctt ttatgccata aaacatattg taagagaata ccactctttt
      241 tattccttct ttccttcttg tacgtttttt gctgtgagta ggtcgtggtg ctggtgttgc
      301 agttgaaata acttaaaata taaatcataa aactcaaaca taaacttgac tatttattta
      361 tttattaaga aaggaggtac cggtatggcc aagcctttgt ctcaagaaga atccaccctc
      421 attgaaagag caacggctac aatcaacagc atccccatct ctgaagacta cagcgtcgcc
      481 agcgcagctc tctctagcga cggccgcatc ttcactggtg tcaatgtata tcattttact
      541 gggggacctt gcgcagaact cgtggtgctg ggcactgctg ctgctgcggc agctggcaac
      601 ctgacttgta tcgtcgcgat cggaaatgag aacaggggca tcttgagccc ctgcggacgg
      661 tgccgacagg ttcttctcga tctgcatcct gggatcaaag ccatagtgaa ggacagtgat
      721 ggacagccga cggcagttgg gattcgtgaa ttgctgccct ctggttatgt gtgggagggc
      781 tagaaattat taaaaataaa actttaaaaa taaatttgtt tctaacatat ggaagttcct
      841 atactttcta gagaatagga acttcatctt tctagaagat ctcctacaat attctcagct
      901 gccatggaaa atcgatgttc ttc
//
