LOCUS       sequence-82640-e         807 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  serves as PCR-template to generate HR donors for C-terminal 
            Twin-Strep tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 807)
  AUTHORS   Bottcher R, Hollmann M, Merk K, Nitschko V, Obermaier C, 
            Philippou-Massier J, Wieland I, Gaul U, Forstemann K
  TITLE     Efficient chromosomal gene modification with CRISPR/cas9 and 
            PCR-based homologous recombination donors in cultured Drosophila 
            cells.
  JOURNAL   Nucleic Acids Res. 2014 Apr 19.
  PUBMED    24748663
REFERENCE   2  (bases 1 to 807)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2014 Apr 19."
COMMENT     Sequence Label: pIW1
FEATURES             Location/Qualifiers
     source          1..807
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             15..38
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             75..98
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     protein_bind    complement(108..141)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 ggctcgagag cgcttggagc cacccgcagt tcgagaaagg tggaggttcc ggaggtggat
       61 cgggaggtgg atcgtggagc cacccgcagt tcgaaaaatg aactagtgaa gttcctatac
      121 tttctagaga ataggaactt caaataaaca tatgctgttg gaatatacta ttcaacctac
      181 aaaaataacg ttaaacaaca ctactttata tttgatatga atggccacac cttttatgcc
      241 ataaaacata ttgtaagaga ataccactct ttttattcct tctttccttc ttgtacgttt
      301 tttgctgtga gtaggtcgtg gtgctggtgt tgcagttgaa ataacttaaa atataaatca
      361 taaaactcaa acataaactt gactatttat ttatttatta agaaaggagg taccggtatg
      421 gccaagcctt tgtctcaaga agaatccacc ctcattgaaa gagcaacggc tacaatcaac
      481 agcatcccca tctctgaaga ctacagcgtc gccagcgcag ctctctctag cgacggccgc
      541 atcttcactg gtgtcaatgt atatcatttt actgggggac cttgcgcaga actcgtggtg
      601 ctgggcactg ctgctgctgc ggcagctggc aacctgactt gtatcgtcgc gatcggaaat
      661 gagaacaggg gcatcttgag cccctgcgga cggtgccgac aggttcttct cgatctgcat
      721 cctgggatca aagccatagt gaaggacagt gatggacagc cgacggcagt tgggattcgt
      781 gaattgctgc cctctggtta tgtgtgg
//
