LOCUS       sequence-82641-e        8236 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To create stable cell clones with high-level expression of Cas9 and 
            OCT1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8236)
  AUTHORS   Zhou Y, Zhu S, Cai C, Yuan P, Li C, Huang Y, Wei W
  TITLE     High-throughput screening of a CRISPR/Cas9 library for functional 
            genomics in human cells.
  JOURNAL   Nature. 2014 Apr 9. doi: 10.1038/nature13166.
  PUBMED    24717434
REFERENCE   2  (bases 1 to 8236)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2014 Apr 9. doi: 10.1038/nature13166."
COMMENT     Sequence Label: pLenti-OC-IRES-BSD
FEATURES             Location/Qualifiers
     source          1..8236
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     enhancer        21..324
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        325..528
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     479..499
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     525..549
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2905..2961
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             2968..2988
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3013..7116
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             3013..7116
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             3013..7116
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             7117..7164
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     misc_feature    7182..7754
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7348..7365)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7575..7594
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     promoter        7771..7818
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7837..8235
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
ORIGIN
        1 cgataagctt gggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
       61 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
      121 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
      181 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
      241 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
      301 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
      361 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
      421 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
      481 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac
      541 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag acaccgactc
      601 tagaggatcc ggactagttt accggtgggg gcccccggcg cgccggtgta caccctgcag
      661 gggtttatga acaatccgtc agaaaccagt aaaccatcta tggagagtgg agatggcaac
      721 acaggcacac aaaccaatgg tctggacttt cagaagcagc ctgtgcctgt aggaggagca
      781 atctcaacag cccaggcgca ggctttcctt ggacatctcc atcaggtcca actcgctgga
      841 acaagtttac aggctgctgc tcagtcttta aatgtacagt ctaaatctaa tgaagaatcg
      901 ggggattcgc agcagccaag ccagccttcc cagcagcctt cagtgcaggc agccattccc
      961 cagacccagc ttatgctagc tggaggacag ataactgggc ttactttgac gcctgcccag
     1021 caacagttac tactccagca ggcacaggca caggcacagc tgctggctgc tgcagtgcag
     1081 cagcactccg ccagccagca gcacagtgct gctggagcca ccatctccgc ctctgctgcc
     1141 acgcccatga cgcagatccc cctgtctcag cccatacaga tcgcacagga tcttcaacaa
     1201 ctgcaacagc ttcaacagca gaatctcaac ctgcaacagt ttgtgttggt gcatccaacc
     1261 accaatttgc agccagcgca gtttatcatc tcacagacgc cccagggcca gcagggtctc
     1321 ctgcaagcgc aaaatcttct aacgcaacta cctcagcaaa gccaagccaa cctcctacag
     1381 tcgcagccaa gcatcaccct cacctcccag ccagcaaccc caacacgcac aatagcagca
     1441 accccaattc agacacttcc acagagccag tcaacaccaa agcgaattga tactcccagc
     1501 ttggaggagc ccagtgacct tgaggagctt gagcagtttg ccaagacctt caaacaaaga
     1561 cgaatcaaac ttggattcac tcagggtgat gttgggctcg ctatggggaa actatatgga
     1621 aatgacttca gccaaactac catctctcga tttgaagcct tgaacctcag ctttaagaac
     1681 atgtgcaagt tgaagccact tttagagaag tggctaaatg atgcagagaa cctctcatct
     1741 gattcgtccc tctccagccc aagtgccctg aattctccag gaattgaggg cttgagccgt
     1801 aggaggaaga aacgcaccag catagagacc aacatccgtg tggccttaga gaagagtttc
     1861 ttggagaatc aaaagcctac ctcggaagag atcactatga ttgctgatca gctcaatatg
     1921 gaaaaagagg tgattcgtgt ttggttctgt aaccgccgcc agaaagaaaa aagaatcaac
     1981 ccaccaagca gtggtgggac cagcagctca cctattaaag caattttccc cagcccaact
     2041 tcactggtgg cgaccacacc aagccttgtg actagcagtg cagcaactac cctcacagtc
     2101 agccctgtcc tccctctgac cagtgctgct gtgacgaatc tttcagttac aggcacttca
     2161 gacaccacct ccaacaacac agcaaccgtg atttccacag cgcctccagc ttcctcagca
     2221 gtcacgtccc cctctctgag tccctcccct tctgcctcag cctccacctc cgaggcatcc
     2281 agtgccagtg agaccagcac aacacagacc acctccactc ctttgtcctc ccctcttggg
     2341 accagccagg tgatggtgac agcatcaggt ttgcaaacag cagcagctgc tgcccttcaa
     2401 ggagctgcac agttgccagc aaatgccagt cttgctgcca tggcagctgc tgcaggacta
     2461 aacccaagcc tgatggcacc ctcacagttt gcggctggag gtgccttact cagtctgaat
     2521 ccagggaccc tgagcggtgc tctcagccca gctctaatga gcaacagtac actggcaact
     2581 attcaagctc ttgcttctgg tggctctctt ccaataacat cacttgatgc aactgggaac
     2641 ctggtatttg ccaatgcggg aggagccccc aacatcgtga ctgcccctct gttcctgaac
     2701 cctcagaacc tctctctgct caccagcaac cctgttagct tggtctctgc cgccgcagca
     2761 tctgcaggga actctgcacc tgtagccagc cttcacgcca cctccacctc tgctgagtcc
     2821 atccagaact ctctcttcac agtggcctct gccagcgggg ctgcgtccac caccaccacc
     2881 gcctccaagg cacagggaag cggagctact aacttcagcc tgctgaagca ggctggagac
     2941 gtggaggaga accctggacc tatggcccca aagaagaagc ggaaggtcgg tatccacggt
     3001 gtcccagcag ccatggacaa gaagtactcc attgggctcg atatcggcac aaacagcgtc
     3061 ggctgggccg tcattacgga cgagtacaag gtgccgagca aaaaattcaa agttctgggc
     3121 aataccgatc gccacagcat aaagaagaac ctcattggcg ccctcctgtt cgactccggg
     3181 gagacggccg aagccacgcg gctcaaaaga acagcacggc gcagatatac ccgcagaaag
     3241 aatcggatct gctacctgca ggagatcttt agtaatgaga tggctaaggt ggatgactct
     3301 ttcttccata ggctggagga gtcctttttg gtggaggagg ataaaaagca cgagcgccac
     3361 ccaatctttg gcaatatcgt ggacgaggtg gcgtaccatg aaaagtaccc aaccatatat
     3421 catctgagga agaagcttgt agacagtact gataaggctg acttgcggtt gatctatctc
     3481 gcgctggcgc atatgatcaa atttcgggga cacttcctca tcgaggggga cctgaaccca
     3541 gacaacagcg atgtcgacaa actctttatc caactggttc agacttacaa tcagcttttc
     3601 gaagagaacc cgatcaacgc atccggagtt gacgccaaag caatcctgag cgctaggctg
     3661 tccaaatccc ggcggctcga aaacctcatc gcacagctcc ctggggagaa gaagaacggc
     3721 ctgtttggta atcttatcgc cctgtcactc gggctgaccc ccaactttaa atctaacttc
     3781 gacctggccg aagatgccaa gcttcaactg agcaaagaca cctacgatga tgatctcgac
     3841 aatctgctgg cccagatcgg cgaccagtac gcagaccttt ttttggcggc aaagaacctg
     3901 tcagacgcca ttctgctgag tgatattctg cgagtgaaca cggagatcac caaagctccg
     3961 ctgagcgcta gtatgatcaa gcgctatgat gagcaccacc aagacttgac tttgctgaag
     4021 gcccttgtca gacagcaact gcctgagaag tacaaggaaa ttttcttcga tcagtctaaa
     4081 aatggctacg ccggatacat tgacggcgga gcaagccagg aggaatttta caaatttatt
     4141 aagcccatct tggaaaaaat ggacggcacc gaggagctgc tggtaaagct taacagagaa
     4201 gatctgttgc gcaaacagcg cactttcgac aatggaagca tcccccacca gattcacctg
     4261 ggcgaactgc acgctatact caggcggcaa gaggatttct accccttttt gaaagataac
     4321 agggaaaaga ttgagaaaat cctcacattt cggataccct actatgtagg ccccctcgcc
     4381 cggggaaatt ccagattcgc gtggatgact cgcaaatcag aagagaccat cactccctgg
     4441 aacttcgagg aagtcgtgga taagggggcc tctgcccagt ccttcatcga aaggatgact
     4501 aactttgata aaaatctgcc taacgaaaag gtgcttccta aacactctct gctgtacgag
     4561 tacttcacag tttataacga gctcaccaag gtcaaatacg tcacagaagg gatgagaaag
     4621 ccagcattcc tgtctggaga gcagaagaaa gctatcgtgg acctcctctt caagacgaac
     4681 cggaaagtta ccgtgaaaca gctcaaagaa gactatttca aaaagattga atgtttcgac
     4741 tctgttgaaa tcagcggagt ggaggatcgc ttcaacgcat ccctgggaac gtatcacgat
     4801 ctcctgaaaa tcattaaaga caaggacttc ctggacaatg aggagaacga ggacattctt
     4861 gaggacattg tcctcaccct tacgttgttt gaagataggg agatgattga agaacgcttg
     4921 aaaacttacg ctcatctctt cgacgacaaa gtcatgaaac agctcaagag gcgccgatat
     4981 acaggatggg ggcggctgtc aagaaaactg atcaatggga tccgagacaa gcagagtgga
     5041 aagacaatcc tggattttct taagtccgat ggatttgcca accggaactt catgcagttg
     5101 atccatgatg actctctcac ctttaaggag gacatccaga aagcacaagt ttctggccag
     5161 ggggacagtc ttcacgagca catcgctaat cttgcaggta gcccagctat caaaaaggga
     5221 atactgcaga ccgttaaggt cgtggatgaa ctcgtcaaag taatgggaag gcataagccc
     5281 gagaatatcg ttatcgagat ggcccgagag aaccaaacta cccagaaggg acagaagaac
     5341 agtagggaaa ggatgaagag gattgaagag ggtataaaag aactggggtc ccaaatcctt
     5401 aaggaacacc cagttgaaaa cacccagctt cagaatgaga agctctacct gtactacctg
     5461 cagaacggca gggacatgta cgtggatcag gaactggaca tcaatcggct ctccgactac
     5521 gacgtggatc atatcgtgcc ccagtctttt ctcaaagatg attctattga taataaagtg
     5581 ttgacaagat ccgataaaaa tagagggaag agtgataacg tcccctcaga agaagttgtc
     5641 aagaaaatga aaaattattg gcggcagctg ctgaacgcca aactgatcac acaacggaag
     5701 ttcgataatc tgactaaggc tgaacgaggt ggcctgtctg agttggataa agcaggcttc
     5761 atcaaaaggc agcttgttga gacacgccag atcaccaagc acgtggccca aattctcgat
     5821 tcacgcatga acaccaagta cgatgaaaat gacaaactga ttcgagaggt gaaagttatt
     5881 actctgaagt ctaagctggt ctcagatttc agaaaggact ttcagtttta taaggtgaga
     5941 gagatcaaca attaccacca tgcgcatgat gcctacctga atgcagtggt aggcactgca
     6001 cttatcaaaa aatatcccaa gcttgaatct gaatttgttt acggagacta taaagtgtac
     6061 gatgttagga aaatgatcgc aaagtctgag caggaaatag gcaaggccac cgctaagtac
     6121 ttcttttaca gcaatattat gaattttttc aagaccgaga ttacactggc caatggagag
     6181 attcggaagc gaccacttat cgaaacaaac ggagaaacag gagaaatcgt gtgggacaag
     6241 ggtagggatt tcgcgacagt ccggaaggtc ctgtccatgc cgcaggtgaa catcgttaaa
     6301 aagaccgaag tacagaccgg aggcttctcc aaggaaagta tcctcccgaa aaggaacagc
     6361 gacaagctga tcgcacgcaa aaaagattgg gaccccaaga aatacggcgg attcgattct
     6421 cctacagtcg cttacagtgt actggttgtg gccaaagtgg agaaagggaa gtctaaaaaa
     6481 ctcaaaagcg tcaaggaact gctgggcatc acaatcatgg agcgatcaag cttcgaaaaa
     6541 aaccccatcg actttctcga ggcgaaagga tataaagagg tcaaaaaaga cctcatcatt
     6601 aagcttccca agtactctct ctttgagctt gaaaacggcc ggaaacgaat gctcgctagt
     6661 gcgggcgagc tgcagaaagg taacgagctg gcactgccct ctaaatacgt taatttcttg
     6721 tatctggcca gccactatga aaagctcaaa gggtctcccg aagataatga gcagaagcag
     6781 ctgttcgtgg aacaacacaa acactacctt gatgagatca tcgagcaaat aagcgaattc
     6841 tccaaaagag tgatcctcgc cgacgctaac ctcgataagg tgctttctgc ttacaataag
     6901 cacagggata agcccatcag ggagcaggca gaaaacatta tccacttgtt tactctgacc
     6961 aacttgggcg cgcctgcagc cttcaagtac ttcgacacca ccatagacag aaagcggtac
     7021 acctctacaa aggaggtcct ggacgccaca ctgattcatc agtcaattac ggggctctat
     7081 gaaacaagaa tcgacctctc tcagctcggt ggagacaagc gtcctgctgc tactaagaaa
     7141 gctggtcaag ctaagaaaaa gaaataatta ataaaattcc gcccctctcc ctcccccccc
     7201 ctaacgttac tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc tatatgtgat
     7261 tttccaccat attgccgtct tttggcaatg tgagggcccg gaaacctggc cctgtcttct
     7321 tgacgagcat tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg
     7381 tcgtgaagga agcagttcct ctggaagctt cttgaagaca aacaacgtct gtagcgaccc
     7441 tttgcaggca gcggaacccc ccacctggcg acaggtgcct ctgcggccaa aagccacgtg
     7501 tataagatac acctgcaaag gcggcacaac cccagtgcca cgttgtgagt tggatagttg
     7561 tggaaagagt caaatggctc tcctcaagcg tattcaacaa ggggctgaag gatgcccaga
     7621 aggtacccca ttgtatggga tctgatctgg ggcctcggtg cacatgcttt acatgtgttt
     7681 agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa
     7741 aaacacgatg ataagctagc tcgagcacgt gttgacaatt aatcatcggc atagtatatc
     7801 ggcatagtat aatacgacaa ggtgaggaac taaaccatgg ccaagccttt gtctcaagaa
     7861 gaatccaccc tcattgaaag agcaacggct acaatcaaca gcatccccat ctctgaagac
     7921 tacagcgtcg ccagcgcagc tctctctagc gacggccgca tcttcactgg tgtcaatgta
     7981 tatcatttta ctgggggacc ttgtgcagaa ctcgtggtgc tgggcactgc tgctgctgcg
     8041 gcagctggca acctgacttg tatcgtcgcg atcggaaatg agaacagggg catcttgagc
     8101 ccctgcggac ggtgccgaca ggtgcttctc gatctgcatc ctgggatcaa agccatagtg
     8161 aaggacagtg atggacagcc gacggcagtt gggattcgtg aattgctgcc ctctggttat
     8221 gtgtgggagg gctaag
//
