LOCUS       sequence-82643-e         616 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Double-GFP reporter for DNA cleavage.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 616)
  AUTHORS   Bottcher R, Hollmann M, Merk K, Nitschko V, Obermaier C, 
            Philippou-Massier J, Wieland I, Gaul U, Forstemann K
  TITLE     Efficient chromosomal gene modification with CRISPR/cas9 and 
            PCR-based homologous recombination donors in cultured Drosophila 
            cells.
  JOURNAL   Nucleic Acids Res. 2014 Apr 19.
  PUBMED    24748663
REFERENCE   2  (bases 1 to 616)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2014 Apr 19."
COMMENT     Sequence Label: pKF251
FEATURES             Location/Qualifiers
     source          1..616
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 aaggaatagt attctgagtg tcgtattgag tctgagtgag acagcgatat gattgttgat
       61 taacccttag catgtccgtg gggtttgaat taactcataa tattaattag acgaaattat
      121 ttttaaagtt ttatttttaa taatttgcga gtacgcaaag cttggctgca gtgtcgccgg
      181 aacgcagcga cagagattcc aatgtgtccg tatctttcag gcttttgccc ttcagttcca
      241 gacgaagcga ctggcgattc gcgtgtgggg tctgcttcag ggtcttgtga attagggcgc
      301 gcagatcgcc gatgggcgtg gcgccggagg gcaccttcac cttgccgtac ggcttgctgt
      361 tcttcgcgtt caaaatctcc agctccattt tgctttcggt gcgcttgcaa tcagtactgt
      421 ccaaaatcga aaatcgccga accgtagtgt gaccgtgcgg ggctctgcga aaataaactt
      481 ttttaggtat atggccacac acggggaaag cacagtggat tatatgtttt aatattataa
      541 tatgcaggtt ttcattactt atccagatgt aagcccactt aaagcgattt aacaattatt
      601 tgccgaaaga gtataa
//
