LOCUS       sequence-82644-e         950 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Please note this plasmid is discontinued. If you need this plasmid 
            for experiments, please directly contact Robert Campbell at 
            University of Alberta..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 950)
  AUTHORS   Belal AS, Sell BR, Hoi H, Davidson MW, Campbell RE
  TITLE     Optimization of a genetically encoded biosensor for cyclin B1-cyclin
            dependent kinase 1.
  JOURNAL   Mol Biosyst. 2014 Feb;10(2):191-5. doi: 10.1039/c3mb70402e.
  PUBMED    24281384
REFERENCE   2  (bases 1 to 950)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Mol Biosyst"; date: "2014-02"; volume: "10"; issue: "2"; pages: 
            "191-5"
COMMENT     Sequence Label: cyclin B1-CDK1 FRET biosensor
FEATURES             Location/Qualifiers
     source          1..950
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(455..457,458..460,461..919)
                     /codon_start=1
                     /product="improved N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN155(I152L)
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMA"
     primer_bind     complement(500..521)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(761..780)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ttgtaaaatt gaagacaata ggttgtcacg agttcattgc ttcattttca aaaagaggca
       61 tgctgtaggc aaaagcatgt atgaatctcc ggcacaaggt ttagatgata tttggtattg
      121 ccacaccgga actaacgtga gctatttaaa taataaccgc atgatacagg gtacgaaatt
      181 ccttttacaa gacggagatg aaatcaagat catttgggat aaaaacaata aatttgtcat
      241 tggctttaaa gtggaaatta acgatactac aggtctgttt aacgagggat taggtatgtt
      301 acaagaacaa agagtagtac ttaagcaaac agccgaagaa aaagatttgg tgaaaaagtt
      361 agtcgacggc ggcagcggcg gcggcggcag cggcggcacc cctgagccta ttttggttga
      421 tacttcctct ccaagcccaa tggaaacagg taccatggtg agcaagggcg aggagctgtt
      481 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
      541 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg
      601 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccttcg gctacggcct
      661 gatgtgcttc gcccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
      721 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
      781 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
      841 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca
      901 caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact
//
