LOCUS       sequence-82646-e         757 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses I-SceI nuclease in Drosophila.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 757)
  AUTHORS   Bottcher R, Hollmann M, Merk K, Nitschko V, Obermaier C, 
            Philippou-Massier J, Wieland I, Gaul U, Forstemann K
  TITLE     Efficient chromosomal gene modification with CRISPR/cas9 and 
            PCR-based homologous recombination donors in cultured Drosophila 
            cells.
  JOURNAL   Nucleic Acids Res. 2014 Apr 19.
  PUBMED    24748663
REFERENCE   2  (bases 1 to 757)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2014 Apr 19."
COMMENT     Sequence Label: pKF257
FEATURES             Location/Qualifiers
     source          1..757
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atagtattct gagtgtcgta ttgagtctga gtgagacagc gatatgattg ttgattaacc
       61 cttagcatgt ccgtggggtt tgaattaact cataatatta attagacgaa attattttta
      121 aagttttatt tttaataatt tgcgagtacg caaagcttgg ctgcatggcg cgcctttgcg
      181 atcgcagacc taggagactt gtgacaaaat tggatccgac tttattgatt acgttgttaa
      241 gagaacaaat cttttacaac tgaattcatt tgttctcgtt tcattttttt tcgcaaaaca
      301 ttgatcgaga attcgattga tttccgattc gaatgctagg agacttgtga acaaattgga
      361 tccgacttta ttgattacgt tgttaagaga acaaatcttt tacaactgaa ttcatttgtt
      421 ctcgtttcat tttttttcgc aaaacattga tcgagaattc gattgatttc cgattcgaat
      481 gctagctata cgcgttatac cggtatcctg caggtagcat gcaagagatc ttataggcct
      541 cgagttaacg ttcgaggtcg actctagata aagctttaga gctcttctta agaaggatcc
      601 agatccacta gtggcctatg cggccgctta tttcaggaaa gtttcggagg agatagtgtt
      661 cggcagtttg tacatcatct gcgggatcag gtacggtttg atcaggttgt agaagatcag
      721 gtaagacata gaatcgatgt agatgatcgg tttgttt
//
