LOCUS       sequence-82649-e         962 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  An engineered monomeric Zoanthus sp. yellow fluorescent protein 
            called mpapaya.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 962)
  AUTHORS   Hoi H, Howe ES, Ding Y, Zhang W, Baird MA, Sell BR, Allen JR, 
            Davidson MW, Campbell RE
  TITLE     An engineered monomeric Zoanthus sp. yellow fluorescent protein.
  JOURNAL   Chem Biol. 2013 Oct 24;20(10):1296-304. doi: 
            10.1016/j.chembiol.2013.08.008. Epub 2013 Oct 3.
  PUBMED    24094838
REFERENCE   2  (bases 1 to 962)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chembiol.2013.08.008"; journalName: "Chem Biol"; date: 
            "2013-10-24- 24"; volume: "20"; issue: "10"; pages: "1296-304"
COMMENT     Sequence Label: pcDNA3.1 mPapaya1.0
FEATURES             Location/Qualifiers
     source          1..962
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     49..68
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        49..67
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      92..101
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             110..823
                     /codon_start=1
                     /product="monomeric yellow fluorescent protein derived from
                     the Zoanthus fluorescent protein zFP538"
                     /label=mPapaya1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEGQSKHGLKEEMTVKYHMEGCVNGHKFVITGEGIGNPFKG
                     KQTANLCVIEGGPLPFSEDILSPGFKYGDRIFTEYPQDIVDYFKNSCPAGYTWERSYLF
                     EDGAVCRCNVDITVSEKENCIYHKSIFRGVNFPADGPVMKKMTTNWEASTEKIVPVPKQ
                     GILKGKVKMCLLLKDGGRYHCQFDTVYKAKSVPSKMPEWHFIQHKLLREDRSDAKNQKW
                     QLTEHAIAGMDELYK"
     primer_bind     complement(848..865)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 gagctctctg gctactagag aacccactgc ttactggctt atcgaaatta atacgactca
       61 ctatagggag acccaagctg gctagcgtgc cgccaccatg gcctcgagaa tggtgagcaa
      121 gggcgagggg caatcgaagc atggactaaa ggaggagatg acggtgaagt accacatgga
      181 aggttgcgtt aatggtcaca aatttgtcat tactggagag ggcattggaa acccttttaa
      241 gggtaagcaa accgcaaatt tgtgtgtgat agaaggaggc ccgctgccgt tctcggagga
      301 cattttaagt ccgggtttta aatatggtga ccggattttc acagagtacc cacaggatat
      361 tgtagattac ttcaagaact catgtccagc gggctatacg tgggaaagga gctacctctt
      421 tgaggacgga gcggtctgtc gatgcaacgt ggacataaca gtctctgaaa aggagaactg
      481 catctatcac aaaagtatct tcagaggggt gaattttccc gccgacggcc ccgtaatgaa
      541 gaaaatgacc actaattggg aagctagtac cgagaaaatt gtgcctgttc caaagcaagg
      601 gatattaaag ggaaaggtca aaatgtgcct gttgctgaag gatggcggtc gttatcattg
      661 ccagtttgat acggtatata aagctaaatc agtgccctcc aagatgccag aatggcattt
      721 tatacagcat aagctcctta gggaggaccg ctctgatgct aaaaaccaga aatggcaatt
      781 aacagaacat gcaatcgcag gcatggacga gctgtacaag taaaaagctt ggtaaaccgc
      841 tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc cctccccccg
      901 tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa
      961 tt
//
