LOCUS       sequence-82656-e         910 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mCherry-based Calcium indicators.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Carlson HJ, Campbell RE
  TITLE     Circular permutated red fluorescent proteins and calcium ion 
            indicators based on mCherry.
  JOURNAL   Protein Eng Des Sel. 2013 Dec;26(12):763-72. doi: 
            10.1093/protein/gzt052. Epub 2013 Oct 22.
  PUBMED    24151339
REFERENCE   2  (bases 1 to 910)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/protein/gzt052"; journalName: "Protein Eng Des Sel"; date: 
            "2013-12"; volume: "26"; issue: "12"; pages: "763-72"
COMMENT     Sequence Label: CH-GECO2.1
FEATURES             Location/Qualifiers
     source          1..910
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             72..83
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             238..255
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             259..291
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(295..303,304..318)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             346..402
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKAGHAVRAIGRLSS"
     primer_bind     complement(839..857)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 ttttatcgca actctctact gtttctccat acccgttttt tgggctaaca ggaggaatta
       61 accatgggtt taaagaggag aaaggtcatg aacaataacg atctctttca ggcatcacgt
      121 cggcgttttc tggcacaact cggcagctta accgacgccg ggacgctggg gccgtcattg
      181 ttaacgccgc gacgtgcgac tgcggcgcaa gcggcgactg acgcttctag aggttctcat
      241 catcatcatc atcatggtat ggctagcatg actggtggac agcaaatggg tcgggatctg
      301 tacgacgatg atgataagga tctcgccaca atggtcgact catcacgtcg taagtggaat
      361 aaggcaggtc atgcagtcag agctataggt cggctgagct cacttgagtc gctgctgtcc
      421 accgagcggc tttaccccga ggacggcgct ctgaagggcg agatcaagca gaggctgaag
      481 ctgaaggacg gcggccacta cgccgccgag gtcaagacca cctacaaggc caagaagccc
      541 gtgcagctgc ccgacgccta catcgtcgac atcaagttgg acatcgtgtc ccacaacgag
      601 gactacacca tcgtggaaca gtacgaacgc gccgagggcc gccactccac cggcggcatg
      661 gacgagctgt acaagggtgg cactggcggt tccatggtga gcaagggcgt ggaggataac
      721 atggccttca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtcaacggc
      781 cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc
      841 aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag
      901 ttcatgtacg
//
