LOCUS       sequence-82657-e         460 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses splitTVA-EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Kohara K, Pignatelli M, Rivest AJ, Jung HY, Kitamura T, Suh J, Frank
            D, Kajikawa K, Mise N, Obata Y, Wickersham IR, Tonegawa S
  TITLE     Cell type-specific genetic and optogenetic tools reveal hippocampal 
            CA2 circuits.
  JOURNAL   Nat Neurosci. 2014 Feb;17(2):269-79. doi: 10.1038/nn.3614. Epub 2013
            Dec 15.
  PUBMED    24336151
REFERENCE   2  (bases 1 to 460)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nn.3614"; 
            journalName: "Nat Neurosci"; date: "2014-02"; volume: "17"; issue: 
            "2"; pages: "269-79"
COMMENT     Sequence Label: pAAV-synP-FLEX-splitTVA-EGFP
FEATURES             Location/Qualifiers
     source          1..460
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    63..96
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     primer_bind     complement(150..171)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tacggaagtt atgcagaatg gtagctggat tgtagctgct attagcaata tgaaacctct
       61 taataacttc gtataaagta tcctatacga agttatggcg cgccttactt gtacagctcg
      121 tccatgccga gagtgatccc ggcggcggtc acgaactcca gcaggaccat gtgatcgcgc
      181 ttctcgttgg ggtctttgct cagggcggac tgggtgctca ggtagtggtt gtcgggcagc
      241 agcacggggc cgtcgccgat gggggtgttc tgctggtagt ggtcggcgag ctgcacgctg
      301 ccgtcctcga tgttgtggcg gatcttgaag ttcaccttga tgccgttctt ctgcttgtcg
      361 gccatgatat agacgttgtg gctgttgtag ttgtactcca gcttgtgccc caggatgttg
      421 ccgtcctcct tgaagtcgat gcccttcagc tcgatgcggt
//
