LOCUS       sequence-82658-e         950 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A improved cpmCherry variant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 950)
  AUTHORS   Carlson HJ, Campbell RE
  TITLE     Circular permutated red fluorescent proteins and calcium ion 
            indicators based on mCherry.
  JOURNAL   Protein Eng Des Sel. 2013 Dec;26(12):763-72. doi: 
            10.1093/protein/gzt052. Epub 2013 Oct 22.
  PUBMED    24151339
REFERENCE   2  (bases 1 to 950)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/protein/gzt052"; journalName: "Protein Eng Des Sel"; date: 
            "2013-12"; volume: "26"; issue: "12"; pages: "763-72"
COMMENT     Sequence Label: cp196V1.2
FEATURES             Location/Qualifiers
     source          1..950
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             78..95
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             99..131
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(135..143,144..158)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     primer_bind     complement(472..490)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     740..759
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 ctttttatcg caactctcta ctgtttctcc atacccgttt tttgggctaa caggaggaat
       61 taaccatggg gggttctcat catcatcatc atcatggtat ggctagcatg actggtggac
      121 agcaaatggg tcgggatctg tacgacgatg acgataagga tccgagctcg agcatgtcca
      181 gctccggggc actggtctgt tttatcaagt tggacatcac ctcacacaac gaggactaca
      241 ccatcgtgga acagtacgaa cgcgccgagg tccgccactc catcggcggc atggacgagc
      301 tgtacaaggg tggcactggc ggttccatgg tgagcaaggg cgtggaggat aacatggcct
      361 tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg ctccgtcaac ggccacgagt
      421 tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga
      481 aggtgaccaa gggtggcccc ctgcccttcg cctgggacat cctgtcccct cagctcatgt
      541 acggctccaa ggcctacgtg aagcaccccg ccgacatccc cgactactgg aagctgtcct
      601 tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggaggc gtggtgaccg
      661 tgacccagga ctcctccctg caggacggcg agttcatcta caaggtgaag ctgcgcggca
      721 ccaacttccc ctccgacggc cccgtaatgc agaagaagac catgggctgg gaggcctcca
      781 ttgagcggtt gtaccccgag gacggcgctc tgaagggcga gatcaagcag aggctgaagc
      841 tgaaggacgg cggccactac gacgctgagg tcaagaccac ctacaaggcc aagaagcctg
      901 tgcagctgcc cggcgctcac aacgcgggcg gtgaccaact gacagaagag
//
