LOCUS       sequence-8266-ex        3663 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3663)
  AUTHORS   Zarse K, Schulz TJ, Birringer M, Ristow M
  TITLE     Impaired respiration is positively correlated with decreased life 
            span in Caenorhabditis elegans models of Friedreich Ataxia.
  JOURNAL   FASEB J. 2007 Apr . 21(4):1271-5.
  PUBMED    17215485
REFERENCE   2  (bases 1 to 3663)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FASEB J. 
            2007 Apr . 21(4):1271-5."
COMMENT     Sequence Label: frh-1-A
FEATURES             Location/Qualifiers
     source          1..3663
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        19..37
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1054..1070)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1055..1071)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        complement(1096..1114)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1124..1141)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1124..1140)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1133..1155)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1282..1737
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1369..1388)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1579..1600
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1763..1867
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1868..1936,1937..2728)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2086..2105)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2899..3487
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3388..3407
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3641..3658
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 aacctggctt atcgaaatta atacgactca ctatagggag accggcagat ctgatggggc
       61 tcaaatacca cataatttcg catatgagta atattcagaa taacaaagtt ttcagatgct
      121 ctccactatt ctacgaaaca attttgtacg tcgctcattt tcgtcccgga ttttcagtca
      181 aaatgagtac gaaacagcag ctgattcaac attagaaaga ttatcagact atttcgatca
      241 aattgccgat tcatttccgg tttccgaaca atttgatgta tctcatgcaa tgggagtttt
      301 gactgttaat gtatcgaaat ctgttggtac gagatggttc ttgtatttct taaattaatt
      361 acttaatttc aggaacgtat gttattaaca aacaatcacc aaataagcaa atttggttgt
      421 ccagtccgat gtctggtccg aaaagatatg atttagaaga ggagggaaag ttagtaaaaa
      481 cacattttca ttatttaaat tgtatatctt gatcaaaagc acaactccac tagaccgaat
      541 atgatcaaga tgatcgtgta acgaaaggtt tcacgaacat caaaagagaa gatttagata
      601 tatatcagtt tgcagacaaa tgagattaag ccgagatgag taacatgcaa gtgtcttatt
      661 gcgtaaagtg cagaaaagag gcggagagga tgagcacaaa ttaacgcttt ttgatttcag
      721 atggacttat gcacacgatg gtgaacaact tgactcgctt ctaaacagag aattccgaaa
      781 gattctggcc gatgaccgaa tcgatttctc gcgacatgtc taatccggat ttcatatcat
      841 gtgatttcta ttctctctcg atttttctct ttttcttgca tatttgatgt atcatgtttt
      901 ctcatgtatt gtaacttagt tatatattgt ctagatactt ttctggtaaa gtttttcttg
      961 cccaagcgtt ttttatacgt ttaatgaaaa tgaaactgtg gaaaaatctc tgattattat
     1021 caaaaagtgc tgacggattc ccatcaagct tatcgatacc gtcgacctcg agggggggcc
     1081 cggtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc gtcgttttac
     1141 aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
     1201 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
     1261 gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     1321 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     1381 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
     1441 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
     1501 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     1561 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     1621 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
     1681 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg
     1741 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     1801 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     1861 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     1921 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     1981 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     2041 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     2101 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     2161 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     2221 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     2281 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     2341 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     2401 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     2461 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     2521 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     2581 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     2641 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     2701 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     2761 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     2821 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     2881 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     2941 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     3001 tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc
     3061 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     3121 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     3181 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     3241 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     3301 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     3361 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     3421 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     3481 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     3541 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     3601 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
     3661 agc
//
