LOCUS       sequence-82664-e         891 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Flag-tagged YTHDF2 N-terminal domain, made by mutating to E384(GAA) 
            to stop codon(TAA).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 891)
  AUTHORS   Wang X, Lu Z, Gomez A, Hon GC, Yue Y, Han D, Fu Y, Parisien M, Dai 
            Q, Jia G, Ren B, Pan T, He C
  TITLE     N6-methyladenosine-dependent regulation of messenger RNA stability.
  JOURNAL   Nature. 2014 Jan 2;505(7481):117-20. doi: 10.1038/nature12730. Epub 
            2013 Nov 27.
  PUBMED    24284625
REFERENCE   2  (bases 1 to 891)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature12730"; journalName: "Nature"; date: "2014-01-2- 2"; 
            volume: "505"; issue: "7481"; pages: "117-20"
COMMENT     Sequence Label: pcDNA3-flag-YTHDF2-N
FEATURES             Location/Qualifiers
     source          1..891
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             106..129
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gcagagctct ctggctaact agagaaccca ctgcttactg gcttatcgaa attaatacga
       61 ctcactatag ggagacccaa gcttggtatc gagctcggat ccatggatta caaggacgac
      121 gatgacaaga tgtcggccag cagcctcttg gagcagagac caaaaggtca aggaaacaaa
      181 gtacaaaatg gatctgtaca tcaaaaggat ggattaaacg atgatgattt tgaaccttac
      241 ttgagtccac aggcaaggcc caataatgca tatactgcca tgtcagattc ctacttaccc
      301 agttactaca gtccctccat tggcttctcc tattctttgg gtgaagctgc ttggtctacg
      361 gggggtgaca cagccatgcc ctacttaact tcttatggac agctgagcaa cggagagccc
      421 cacttcctac cagatgcaat gtttgggcaa ccaggagccc taggtagcac tccatttctt
      481 ggtcagcatg gttttaattt ctttcccagt gggattgact tctcagcatg gggaaataac
      541 agttctcagg gacagtctac tcagagctct ggatatagta gcaattatgc ttatgcacct
      601 agctccttag gtggagccat gattgatgga cagtcagctt ttgccaatga gaccctcaat
      661 aaggctcctg gcatgaatac tatagaccaa gggatggcag cactgaagtt gggtagcaca
      721 gaagttgcaa gcaatgttcc aaaagttgta ggttctgctg ttggtagcgg gtccattact
      781 agtaacatcg tggcttccaa tagtttgcct ccagccacca ttgctcctcc aaaaccagca
      841 tcttgggctg atattgctag caagcctgca aaacagcaac ctaaactgaa g
//
