LOCUS       sequence-82668-e         809 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Co-expresses HA tagged channelrhodopsin and hM4D (Gi).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 809)
  AUTHORS   Stachniak TJ, Ghosh A, Sternson SM
  TITLE     Chemogenetic synaptic silencing of neural circuits localizes a 
            hypothalamus-->midbrain pathway for feeding behavior.
  JOURNAL   Neuron. 2014 May 21;82(4):797-808. doi: 
            10.1016/j.neuron.2014.04.008. Epub 2014 Apr 24.
  PUBMED    24768300
REFERENCE   2  (bases 1 to 809)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2014.04.008"; journalName: "Neuron"; date: 
            "2014-05-21- 21"; volume: "82"; issue: "4"; pages: "797-808"
COMMENT     Sequence Label: CAG:: ChR2HA-2a-hM4D
FEATURES             Location/Qualifiers
     source          1..809
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(32..51)
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     intron          34..166
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
ORIGIN
        1 aaattttgta atccagaggt tgattgtcga cacctgtgga gagaaaggca aagtggatgt
       61 cagtaagacc aataggtgcc tatcagaaac gcaagagtct tctctgtctc gacaagccca
      121 gtttctattg gtctccttaa acctgtcttg taaccttgat acttacctgc ggcctcgacc
      181 ttagctattc atcggtacct cgagcaccgg tacgcgtgat cgatgcataa cctacctggc
      241 agtgccgatg ttccgatact ggcacagcag caggtgccgg aaggtctttt taaaggtggc
      301 gttgcacaga gcatagcagg cagggttgat ggtgctgttg acgtagcaga gccagtagcc
      361 aatggaccac accgtgtcag ggatgcagct ctggcagaag gtgttcacca ggaccatgac
      421 gttgtagggc gtccaggtga ggatgaaggc tagcagaatg gcaaagatcg ttcgtgtcac
      481 tttgcgctcc cgggccgcca tctgccgctt cttgcgcacc tggttgcgag cgatgctggc
      541 gaacttgcgg gccacgttgg ccgcagggcg catgccagcc ggcgtggcag gcacaatctc
      601 aatggctgtc acacactcat tgcctgtctg cttcgtcaca atctggatct tggaccatct
      661 ggaggctggg ttgagggccc gcggctgcag gggaggggcg ggcatggcgg gcgtggtggc
      721 ctctgtggtg gacagctctg tggctgggcg ttccttggtg ttctgggtgg cactgcctga
      781 gctggactca ttggaagtgt ccttatcaa
//
