LOCUS       sequence-82677-e         829 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  lentiviral U6 driven sgRNA cloning vector where guide sequences are 
            inserted between BfuAI sites, improved cassette cloning efficiency.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 829)
  TITLE     Improved U6-sgRNA vector for expression
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLKO.1-puro U6 sgRNA BfuAI large stuffer
FEATURES             Location/Qualifiers
     source          1..829
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     121..140
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(440..459)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
ORIGIN
        1 gttagagaga taattggaat taatttgact gtaaacacaa agatattagt acaaaatacg
       61 tgacgtagaa agtaataatt tcttgggtag tttgcagttt taaaattatg ttttaaaatg
      121 gactatcata tgcttaccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg
      181 tggaaaggac gaaacaccgg tgtgcaggtg agtgatccaa acgcccggcg gcaaccgagc
      241 gttctgaaca aatccagatg gagttctgag gtcattactg gatctatcaa caggagtcca
      301 agcgagctct cgaaccccag agtcccgctc agaagaactc gtcaagaagg cgatagaagg
      361 cgatgcgctg cgaatcggga gcggcgatac cgtaaagcac gaggaagcgg tcagcccatt
      421 cgccgccaag ctcttcagca atatcacggg tagccaacgc tatgtcctga tagcggtccg
      481 ccacacccag ccggccacag tcgatgaatc cagaaaagcg gccattttcc accatgatat
      541 tcggcaagca ggcatcgcca tgggtcacga cgagatcctc gccgtcgggc atgcgcgcct
      601 tgagcctggc gaacagttcg gctggcgcga gcccctgatg ctcttcgtcc agatcatcct
      661 gatcgacaag accggcttcc atccgagtac gtgctcgctc gatgcgatgt ttcgcttggt
      721 ggtcgaatgg gcaggtagcc ggatcaagcg tatgcagccg ccgcattgca tcagccatga
      781 tggatacttt ctcggcagga gcaaggtgag atgacaggag atcctgccc
//
