LOCUS       sequence-82678-e         712 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Co-expresses HA tagged channelrhodopsin and hM4D (Gi).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 712)
  AUTHORS   Stachniak TJ, Ghosh A, Sternson SM
  TITLE     Chemogenetic synaptic silencing of neural circuits localizes a 
            hypothalamus-->midbrain pathway for feeding behavior.
  JOURNAL   Neuron. 2014 May 21;82(4):797-808. doi: 
            10.1016/j.neuron.2014.04.008. Epub 2014 Apr 24.
  PUBMED    24768300
REFERENCE   2  (bases 1 to 712)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2014.04.008"; journalName: "Neuron"; date: 
            "2014-05-21- 21"; volume: "82"; issue: "4"; pages: "797-808"
COMMENT     Sequence Label: CAG:: ChR2HA-2a-hM4D
FEATURES             Location/Qualifiers
     source          1..712
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             48..74
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             48..74
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             75..101
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 aggtcgagac tctcgtcgaa gacgaagccg aggccggagc cgtggcatac ccctacgacg
       61 tgcccgacta cgcctatcca tatgatgtcc cagactatgc tgctagcgaa ggacgtggat
      121 ctctgctgac ctgtggagat ggggaagaga atcccggacc atcgcgaatg gccaacttca
      181 cacctgtcaa tggcagctcg ggcaatcagt ccgtgcgcct ggtcacgtca tcatcccaca
      241 atcgctatga gacggtggaa atggtcttca ttgccacagt gacaggctcc ctgagcctgg
      301 tgactgtcgt gggcaacatc ctggtgatgc tgtccatcaa ggtcaacagg cagctgcaga
      361 cagtcaacaa ctacttcctc ttcagcctgg cgtgtgctga tctcatcata ggcgccttct
      421 ccatgaacct ctacaccgtg tacatcatca agggctactg gcccctgggc gccgtggtct
      481 gcgacctgtg gctggccctg gactgcgtgg tgagcaacgc ctccgtcatg aaccttctca
      541 tcatcagctt tgaccgctac ttctgcgtca ccaagcctct cacctaccct gcccggcgca
      601 ccaccaagat ggcaggcctc atgattgctg ctgcctgggt actgtccttc gtgctctggg
      661 cgcctgccat cttgttctgg cagtttgtgg tgggtaagcg gacggtgccc ga
//
